Identification and analysis of epigenetic mechanisms involved in the maintenance of genome stability

2011 Fiscal Year Final Research Report

• Project/Area Number: 21380204
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Applied molecular and cellular biology
• Research Institution: Tohoku University
• Principal Investigator: HARATA Masahiko 東北大学, 大学院・農学研究科, 准教授 (70218642)
• Co-Investigator(Kenkyū-buntansha): OHTA Kunihiro 東京大学, 総合文化研究科, 教授 (90211789)
• Project Period (FY): 2009 – 2011
• Keywords: クロマチン / ゲノム安定性 / エピジェネティック / アクチン関連タンパク質

Research Abstract

In eukaryotic cells, the genome forms chromatin together with histones, and chromatin is packed into the nucleus in a highly organized manner. These chromatin and nuclear organizations are fundamentals of epigenetic regulation. The maintenance of genome stability is a subject of epigenetic regulation; however, molecular mechanisms involved in the genome stability are largely unknown yet. In this project, we showed that INO80chromatin remodeling complex and histone acetylation complexes play important and evolutionarily conserved roles in the maintenance of the genome stability.

Research Products

• [Journal Article] The actin family member Arp6 and the histone variant H2A.Z are required for spatial positioning of chromatin in chicken cell nuclei (2012)
  • Author(s): Maruyama, E.O., Hori, T., Tanabe, H., Kitamura, H., Matsuda. R., Tone, S., Hozak, P., Habermann, F.A., von Hase, J., Cremer, C., Fukagawa, T., Harata, M
  • Journal Title: J Cell Sci Volume: 125 Pages: 3739-3743
  • Peer Reviewed
• [Journal Article] Actin-related proteins localized in the nucleus: from discovery to novel roles in nuclear organization (2011)
  • Author(s): Oma, Y., Harata, M.
  • Journal Title: Nucleus Volume: 2 Pages: 38-46
  • Peer Reviewed
• [Journal Article] Identification and characterization of two isoforms of the vertebrate H2A (2010)
  • Author(s): Matsuda, R., Hori, T., Kitamura, H., Takeuchi, K., Fukagawa, T., Harata, M
  • Journal Title: Z histone variant. Nucleic Acids Res Volume: 38 Pages: 4263-4673
  • Peer Reviewed
• [Journal Article] Actin-related protein Arp6 influences H2AZ-dependent and -independent gene expression and links ribosomalprotein genes to nuclear pores (2010)
  • Author(s): Yoshida, T, Shimada, K., Oma, Y., Kalck, V., Akimura, K., Taddei, A., Iwahashi, H., Kugou, K., Ohta, K., Gasser, S.M., Harata, M
  • Journal Title: PLoS Genet 6 Pages: e1000910
  • Peer Reviewed
• [Journal Article] The human actin-related protein hArp5: nucleo-cytoplasmic shuttling and involvement in DNA repair Exp Cell (2009)
  • Author(s): Kitayama, K., Kamo, M., Oma, Y., Matsuda, R., Uchida, T., Ikura, T., Tashiro, S., Ohyama, T., Winsor, B., Harata, M
  • Journal Title: Res 315 Pages: 206-217
  • Peer Reviewed
• [Presentation] Interaction between the actin-related protein Arp6 and nuclear myosin I and their contribution to the nuclear organization (2011)
  • Author(s): Hiroshi Kitamura, Eri Ohfuchi, Chikashi Obuse, Hideyuki Tanabe, Tetsuya Hori, Tatsuo Fukagawa, Pavel Hozak, Masahiko Harata
  • Organizer: Wenner-Gren Foundations International Symposium"Actin and actin-associated proteins from gene to polysomes
  • Place of Presentation: Stockholm Sweden(招待講演)
  • Year and Date: 20110907-10
• [Presentation] クロマチン・細胞核の機能構造を介したエピゲノム制御と環境応答 (2011)
  • Author(s): 原田昌彦
  • Organizer: 日本農芸化学会2011年度大会シンポジウムエピゲノムから見た微生物・動物・植物の環境対応とその応用ポテンシャル
  • Place of Presentation: (京都)(招待講演)
  • Year and Date: 2011-03-28
• [Presentation] Roles of actin-related proteins in nuclear organization and genome function (2010)
  • Author(s): Yoshida T, Shimada K, Oma Y, Kugou K, Ohta K, Kitamura H, Tanabe H, Gasser SM, Hozak P, Harata M.
  • Organizer: 52nd Symposium of the Society forHistochemistry
  • Place of Presentation: Prague、Czech Republic(招待講演)
  • Year and Date: 2010-09-01
• [Presentation] 胞核アーキテクチャーにおけるアクチン関連タンパク質の役割 (2009)
  • Author(s): 原田昌彦
  • Organizer: 32回日本分子生物学会年会シンポジウム「細胞核アーキテクチャーに基づくゲノム機能制御:そのコンセプトの最新像
  • Place of Presentation: (横浜)(招待講演)
  • Year and Date: 20091209-12
• [Presentation] 脊椎動物のアクチン関連タンパク質による染色体・細胞核機能構造の形成 (2009)
  • Author(s): 原田昌彦
  • Organizer: 日本遺伝学会第81回大会ワークショップ「核ダイナミクス研究の新展開」
  • Year and Date: 2009-09-17