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2011 Fiscal Year Final Research Report

Molecular mechanisms of import to nuclei, mitochondria, and chloroplast, and UVB resistance in rice.

Research Project

  • PDF
Project/Area Number 21380205
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Applied molecular and cellular biology
Research InstitutionTohoku University

Principal Investigator

HIDEMA Jun  東北大学, 大学院・生命科学研究科, 准教授 (20250855)

Co-Investigator(Kenkyū-buntansha) MIYAZAWA Yutaka  東北大学, 大学院・生命科学研究科, 助教 (00342858)
Project Period (FY) 2009 – 2011
KeywordsCPD光回復酵素 / オルガネラ移行シグナル / UVB抵抗性 / 植物 / 葉緑体 / ミトコンドリア
Research Abstract

Cyclobutane pyrimidine dimer (CPD)photolyase is major pathway for repairing CPD in plant, and CPD photolyase is a crucial factor for determining UVB sensitivity in plants. We found that CPD photolyase, which is encoded by a single-copy gene in the nuclear genome, is expressed and targeted not only to nuclei but also to mitochondria and chloroplasts. To clarify the molecular mechanisms of import to each organelle in rice, the subcellular localization analyses were performed. We found that the amino acids residues from 391 to 401 and from 487 to 489 are functional targeting signal for transport into mitochondria and nuclei, respectively. The results indicate that rice may have evolved a CPD photolyase that functions in all three organelles that contain DNA to protect cells from the harmful effects of UVB radiation.

  • Research Products

    (26 results)

All 2012 2011 2010 2009 Other

All Journal Article (7 results) (of which Peer Reviewed: 7 results) Presentation (17 results) Book (1 results) Remarks (1 results)

  • [Journal Article] Augmentation of CPD photolyase activity in japonica and indica rice increases their UVB resistance but still leaves the difference in their sensitivities2012

    • Author(s)
      Teranishi M, Taguchi T, Ono T, Hidema J
    • Journal Title

      Photochem. Photobiol. Sci

      Volume: 11 Pages: 812-820

    • Peer Reviewed
  • [Journal Article] Eukaryotic Class II CPD photolyase structure reveals a basis for improved UV-tolerance in plants2012

    • Author(s)
      Hitomi K, Arvai AS, Yamamoto J, Hitomi C, Teranishi M, Hirouchi T, Yamamoto K, Iwai S, Tainer JA, Hidema J, Getzoff ED
    • Journal Title

      J Biol Chem

      Volume: 287 Pages: 12060-9

    • Peer Reviewed
  • [Journal Article] CPD photolyase repairs ultraviolet-B-induced CPDs in rice chloroplast and mitochondrial DNA2011

    • Author(s)
      Takahashi M, Teranishi M, Ishida H, Kawasaki J, Takeuchi A, Yamaya T, Watanabe M, Makino A, Hidema J
    • Journal Title

      Plant J

      Volume: 66 Pages: 433-442

    • Peer Reviewed
  • [Journal Article] UV-B induced stress responses in three rice cultivars2010

    • Author(s)
      Fedina I. Hidema J. Velitchkova M. Georgieva K. Nedeva D
    • Journal Title

      Biologia Plantarum

      Volume: 54 Pages: 571-574

    • Peer Reviewed
  • [Journal Article] Visualization of flavonol distribution in the abaxial epidermis o onion scales via detection of its autofluorescence in the absence of chemical processes2009

    • Author(s)
      Sudo E. Teranishi M. Hidema J. Taniuchi T
    • Journal Title

      Bioscience, Biotechnology, and Biochemistry

      Volume: 73 Pages: 2107-2109

    • Peer Reviewed
  • [Journal Article] Betsy and photolyase2009

    • Author(s)
      Hidema J
    • Journal Title

      Biol. Sci. Space

      Volume: 23 Pages: 221-222

    • Peer Reviewed
  • [Journal Article] Light-induced activation of class II cyclobutane pyrimidine dimer photolyase2009

    • Author(s)
      Okafuji A., Biskup T., Hitomi K. GetzoffED., Kaiser G., Bacher A., Hidema J., Teranishi M., Yamamoto K., Schleicher E., Weber S
    • Journal Title

      DNA Repair

      Volume: 9 Pages: 495-505

    • Peer Reviewed
  • [Presentation] イネCPD光回復酵素遺伝子の光発現誘導機構に関する研究2012

    • Author(s)
      廣澤孝奈, 高橋文雄, 寺西美佳, 高野成央, 日出間純
    • Organizer
      第53回日本植物生理学会
    • Place of Presentation
      京都
    • Year and Date
      20120316-18
  • [Presentation] CPD光回復酵素部分置換系統・遺伝子組換えイネを用いた太陽紫外線影響評価試験2012

    • Author(s)
      庄司洸平、山岸朋香、寺西美佳、高野成央、日出間純
    • Organizer
      第53回日本植物生理学会
    • Place of Presentation
      京都
    • Year and Date
      20120316-18
  • [Presentation] イネにおけるクロマチン構造と紫外線誘発DNA損傷に関する研究2012

    • Author(s)
      山田道子、寺西美佳、日出間純
    • Organizer
      第53回日本植物生理学会
    • Place of Presentation
      京都
    • Year and Date
      20120316-18
  • [Presentation] イネのtriple-targetingCPD光回復酵素のオルガネラ移行シグナル配列に関する解析2012

    • Author(s)
      高橋さやか高橋正明寺西美佳日出間純
    • Organizer
      第53回日本植物生理学会
    • Place of Presentation
      京都
    • Year and Date
      20120316-18
  • [Presentation] Hidema Light-Regulated Gene Expression of Rice CPD Photolyase2012

    • Author(s)
      T. Hirosawa, F. Takahashi, M. Teranishi, N. Takano, and J
    • Organizer
      The 1st International Symposium on Plant Environmental Sensing
    • Place of Presentation
      Nara Japan
    • Year and Date
      2012-03-21
  • [Presentation] 高等植物におけるオルガネラDNA修復とUVB抵抗性-CPD光回復酵素の細胞内局在-2012

    • Author(s)
      日出間純
    • Organizer
      第14回植物オルガネラワークショップ-植物オルガネラ研究のマイルストーン
    • Place of Presentation
      京都
    • Year and Date
      2012-03-15
  • [Presentation] UV-induced cyclobutane pyrimidine dimers(CPDs)in all DNA-containing organelles in plant are repaired by CPD photolyase2011

    • Author(s)
      Jun Hidema
    • Organizer
      5th Asia and Oceania Conference for Photobiology
    • Place of Presentation
      Nara. Japan
    • Year and Date
      20110801-02
  • [Presentation] イネCPD光回復酵素の細胞内局在について2011

    • Author(s)
      高橋正明、寺西美佳、石田宏幸、高橋さやか、日出間純
    • Organizer
      第52会日本植物生理学会
    • Place of Presentation
      仙台
    • Year and Date
      20110320-22
  • [Presentation] イネCPD光回復酵素のリン酸化酵素に関する解析2011

    • Author(s)
      古川晴也、寺西美佳、日出間純
    • Organizer
      第52会日本植物生理学会
    • Place of Presentation
      仙台
    • Year and Date
      20110320-22
  • [Presentation] イネCPD光回復酵素のオルガネラ移行シグナル配列に関する解析2011

    • Author(s)
      高橋さやか、高橋正明、寺西美佳、日出間純
    • Organizer
      第52会日本植物生理学会
    • Place of Presentation
      仙台
    • Year and Date
      20110320-22
  • [Presentation] CPD photolyase repairs ultraviolet-B-induced CPDs in rice chloroplast and mitochondrial DNA2011

    • Author(s)
      M. Takahashi, S. Takahashi, M. Teranishi, H. and J. Hidema
    • Organizer
      Societas Physiologia Plantarum Scandinavica 2011
    • Place of Presentation
      Stavanger, Norway
    • Year and Date
      2011-08-23
  • [Presentation] イネCPD光回復酵素の核・ミトコンドリア移行シグナル配列の同定2010

    • Author(s)
      高橋さやか、高橋正明、寺西美佳、日出間純
    • Organizer
      日本放射線影響学会第53回大会
    • Place of Presentation
      京都
    • Year and Date
      20101020-22
  • [Presentation] CPD光回復酵素は、核、葉緑体、ミトコンドリアへ移行し、CPD修復の機能を担っている2010

    • Author(s)
      高橋正明、寺西美佳、石田宏幸、高橋さやか、日出間純
    • Organizer
      日本宇宙生物科学会第24回大会
    • Place of Presentation
      仙台
    • Year and Date
      20100917-18
  • [Presentation] イネCPD光回復酵素の核・ミトコンドリア移行シグナル配列に関する解析2010

    • Author(s)
      高橋さやか、高橋正明、寺西美佳、日出間純
    • Organizer
      第51回日本植物生理学会年会
    • Place of Presentation
      熊本
    • Year and Date
      20100318-21
  • [Presentation] CPD photolyase of rice is phosphorylated2010

    • Author(s)
      M. Teranishi, K. Nakamura, M. Takahashi, J. Hidema
    • Organizer
      Plant DNA Repair and Recombination 2010
    • Place of Presentation
      Asilomar Conference Center, CA, USA
    • Year and Date
      20100302-05
  • [Presentation] CPD photolyase functions in all DNA-containing organelles in rice2010

    • Author(s)
      M. Takahashi, S. Takahashi, M. Teranishi, H. Ishida, J. Kawasaki, A. Takeuchi and J. Hidema
    • Organizer
      Plant DNA Repair and Recombination 2010
    • Place of Presentation
      Asilomar Conference Center, CA, USA
    • Year and Date
      20100302-05
  • [Presentation] CPD photolyase repairs ultraviolet-B-induced CPDs in all DNA-containing organelles in plant2009

    • Author(s)
      M. Takahashi, S. Takahashi, M. Teranishi, H. and J. Hidema
    • Organizer
      Memorial Symposium for 25th international Prize for Biology
    • Place of Presentation
      Kyoto, Japan
    • Year and Date
      20091202-04
  • [Book] からだと光の辞典2011

    • Author(s)
      日出間純
    • Total Pages
      432
    • Publisher
      朝倉書店
  • [Remarks]

    • URL

      http://www.ige.tohoku.ac.jp/genome/index.htm

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Published: 2013-07-31  

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