2011 Fiscal Year Final Research Report
The development of self-assembly nano-devices based on electrostatic interaction and the application to selective drug delivery
Project/Area Number |
21390010
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Research Category |
Grant-in-Aid for Scientific Research (B)
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Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
Physical pharmacy
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Research Institution | Nagasaki University |
Principal Investigator |
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Co-Investigator(Kenkyū-buntansha) |
TO Hideto (90346809)
KAWAKAMI Shigeru (20322307)
KUROSAKI Tomoaki (00582016)
KITAHARA Takashi (30380934)
KODAMA Yukinobu (50448510)
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Project Period (FY) |
2009 – 2011
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Keywords | ドラッグデリバリー / 遺伝子 / 癌 / ナノ材料 |
Research Abstract |
In this study, we developed the self-assembly nano-devices based on electrostatic interaction. We could prepare the stable nano-devices by changing the blend ratio of gene, cationic compounds and anionic compounds and the preparation procedures. The nano-devices containingγ-polyglutamic acid, N-lauroylsarcosine, or glycyrrhizin achieved high intracellular uptake without showing cytotoxicity and blood toxicity. After intravenous administration of these nano-devices in mice, selective high gene expression was observed in the spleen, lung, or liver by the difference of each component. Furthermore, we could mass-produce and freeze-dry nano-devices. In conclusion, we successfully developed the secure and effective nano-devices and revealed the preparation technic responding to therapeutic purpose.
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[Journal Article] Pulmonary gene delivery of hybrid vector, lipopolyplex containing N-lauroylsarcosine, via the systemic route2009
Author(s)
Kurosaki T, Kishikawa R, Matsumoto M, Kodama Y, Hamamoto T, To H, Niidome T, Takayama K, Kitahara T, Sasaki H
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Journal Title
J Control Release
Volume: 136(3)
Pages: 213-219
Peer Reviewed
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[Journal Article] Ternary complexes of pDNA, polyethylenimine, and gamma-polyglutamic acid for gene delivery systems2009
Author(s)
Kurosaki T, Kitahara T, Fumoto S, Nishida K, Nakamura J, Niidome T, Kodama Y, Nakagawa H, To H, Sasaki H
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Journal Title
Biomaterials
Volume: 30(14)
Pages: 2846-2853
Peer Reviewed
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