Molecular mechanisms of the signal transductions by the factors functioning as a trigger for the adipocyte differentiation.

2012 Fiscal Year Final Research Report

• Project/Area Number: 21390024
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Biological pharmacy
• Research Institution: Nagoya City University
• Principal Investigator: IMAGAWA Masayoshi 名古屋市立大学, 大学院・薬学研究科, 教授 (20136823)
• Project Period (FY): 2009 – 2012
• Keywords: 肥満 / 脂肪細胞 / 糖尿病 / 生活習慣病 / シグナル伝達 / 胚形成 / 骨形成 / 肺形成

Research Abstract

Obesity is related to the development of type 2 diabetes mellitus, hypertension, and hyperlipidemia. Obesity results from hypertrophy of individual adipocytes and hyperplasia of adipocytes. Since hyperplasia of adipocytes is caused by the formation of mature adipocytes from preadipocytes, it is important to elucidate the molecular mechanism of adipocyte differentiation. In this study, many genes including novel genes were identified and characterized. Moreover, the knockout mice for two novel genes showed that these genes function as regulators for lung development, bone formation and embryonic development, indicating that these key genes have multiple functions in development.

Research Products

• [Journal Article] Interactions of thyroid hormone receptor with Ku proteins and interleukin enhancer binding factor 3 modulate the promoter activity of thyroid-stimulating hormone alpha. (2012)
  • Author(s): Ohno, M., Fujita, M., Nishizuka, M., Osada, S., and Imagawa, M.
  • Journal Title: Biol. Pharm. Bull Volume: 35 (3) Pages: 380-384
  • URL: https://www.jstage.jst.go.jp/browse/bpb
  • Peer Reviewed
• [Journal Article] Fad158 gene disruption prevents the body weight gain and insulin resistance induced by a high-fat diet. (2011)
  • Author(s): Hayashi, T., Nozaki, Y., Nishizuka, M., Ikawa, M., Osada, S., and Imagawa, M.
  • Journal Title: Biol. Pharm. Bull Volume: 34 (8) Pages: 1257-1263
  • URL: https://www.jstage.jst.go.jp/browse/bpb
  • Peer Reviewed
• [Journal Article] Indispensable role of factor for adipocyte differentiation 104 (fad104) in lung maturation. (2011)
  • Author(s): Kishimoto, K., Nishizuka, M., Ueda, T.,Kajita, K., Ugawa, S., Shimada, S., Osada,S., and Imagawa, M
  • Journal Title: Exp. Cell Res Volume: 317 (15) Pages: 2110-2123
  • DOI: DOI:10.1016/j.yexcr.2011.06.003
  • Peer Reviewed
• [Journal Article] Interleukin enhancer binding factor 3 functions as a liver receptor homolog-1 co-activator in synergy with the nuclear receptor co-activators, PRMT1 and PGC-1α. (2011)
  • Author(s): Ohno, M., Komakine, J., Suzuki, E., Nishizuka, M., Osada S., and Imagawa, M.
  • Journal Title: Biochem. J Volume: 437 (3) Pages: 531-540
  • DOI: DOI:10.1042/BJ20101793
  • Peer Reviewed
• [Journal Article] Fad104, a positive regulator of adipogenesis, negatively regulates osteoblast differentiation (2010)
  • Author(s): Kishimoto, K., Kato, A., Osada, S., Nishizuka, M., and Imagawa, M.
  • Journal Title: Biochem. Biophys. Res. Commun Volume: 397 (2) Pages: 187-191
  • DOI: DOI:10.1016/j.bbrc.2010.05.077
  • Peer Reviewed
• [Journal Article] TCL/TC10・L regulates adipogenesis by controlling mitotic clonal expansion. (2010)
  • Author(s): Kawaji, A., Nishizuka, M., Osada, S., and Imagawa, M.
  • Journal Title: Biol. Pharm. Bull Volume: 33 (3) Pages: 404-409
  • URL: https://www.jstage.jst.go.jp/browse/bpb
  • Peer Reviewed
• [Journal Article] FAD24 causes hyperplasia in adipose tissue and improves glucose metabolism. (2009)
  • Author(s): Johmura, Y., Watanabe, K., Kishimoto, K., Ueda, T., Shimada, S., Osada, S., Nishizuka, M., and Imagawa, M.
  • Journal Title: Biol. Pharm. Bull Volume: 32 (10) Pages: 1656-1664
  • URL: https://www.jstage.jst.go.jp/browse/bpb
  • Peer Reviewed
• [Journal Article] The role of C/EBPδ in the early stages of adipogenesis. (2009)
  • Author(s): Hishida, T., Nishizuka, M., Osada, S., and Imagawa M.
  • Journal Title: Biochimie Volume: 91 Pages: 654-657
  • DOI: DOI:10.1016/j.biochi.2009.02.002
  • Peer Reviewed
• [Journal Article] Disruption of the novel gene fad104 causesrapid postnatal death and attenuation of cell proliferation, adhesion, spreading and migration. (2009)
  • Author(s): Nishizuka, M., Kishimoto, K., Kato, A., Ikawa, M., Okabe, M., Sato, R., Niida, H., Nakanishi, M., Osada, S., and Imagawa, M.
  • Journal Title: Exp. Cell Res Volume: 315 (5) Pages: 809-819
  • DOI: DOI:10.1016/j.yexcr.2008.12.013
  • Peer Reviewed
• [Presentation] 脂肪細胞分化を制御する fad104 が癌細胞の浸潤に与える影響の解析 (2013)
  • Author(s): 加藤大輝、西塚 誠、岸本圭史、長田茂宏、今川正良
  • Organizer: 日本薬学会第133 年会
  • Place of Presentation: 横浜
  • Year and Date: 2013-03-28
• [Presentation] The role of fad104, a regulator of adipogenesis, in calvarial bone formation through BMP/Smad signaling. (2012)
  • Author(s): Keishi Kishimoto, Makoto Nishizuka, Daiki Katoh, Shigehiro Osada, Masayoshi Imagawa
  • Organizer: 2012 American Society Cell Biology (ASCB) Annual Meeting
  • Place of Presentation: San Francisco, CA, USA
  • Year and Date: 2012-12-16
• [Presentation] 脂肪細胞分化制御因子 fad24 はマウス初期胚発生に必須である (2012)
  • Author(s): 落合なつき、桐村拓也、長田茂宏、西塚 誠、今川正良
  • Organizer: 第85回日本生化学会大会
  • Place of Presentation: 福岡
  • Year and Date: 2012-12-16
• [Presentation] 脂肪細胞分化過程における KCNK2 の機能解析 (2012)
  • Author(s): 若林まい、浅野真美、長田茂宏、西塚 誠、今川正良
  • Organizer: 日本病院薬剤師会東海ブロック・日本薬学会東海支部合同学術集会 2012
  • Place of Presentation: 岐阜
  • Year and Date: 2012-11-18
• [Presentation] 癌細胞の移動・浸潤における新規遺伝子fad104 の役割 (2012)
  • Author(s): 加藤大輝、西塚 誠、岸本圭史、長田茂宏、今川正良
  • Organizer: 第11回次世代を担う若手ファーマ・バイオフォーラム 2012
  • Place of Presentation: 京都
  • Year and Date: 2012-09-15
• [Presentation] 脂肪細胞分化に重要な fad104 の骨分化における役割 (2012)
  • Author(s): 岸本圭史、加藤愛友美、長田茂宏、西塚 誠、今川正良
  • Organizer: 日本薬学会第132 年会
  • Place of Presentation: 札幌
  • Year and Date: 2012-03-29
• [Presentation] Novel gene fad104 is required for lung morphogenesis. (2011)
  • Author(s): Keishi Kishimoto, Makoto Nishizuka, Shigehiro Osada, Masayoshi Imagawa
  • Organizer: Experimental Biology 2011
  • Place of Presentation: Washington DC, USA
  • Year and Date: 2011-04-13
• [Presentation] 新規遺伝子 fad24 の生体内における機能解析 (2010)
  • Author(s): 落合なつき、城村由和、岸本圭史、長田茂宏、西塚 誠、今川正良
  • Organizer: 第33回日本分子生物学会年会・第83回日本生化学会大会合同大会
  • Place of Presentation: 神戸
  • Year and Date: 2010-12-10
• [Presentation] 脂肪細胞分化を正に制御する新規遺伝子fad104 の骨分化における役割 (2010)
  • Author(s): 加藤愛友美、岸本圭史、長田茂宏、西塚 誠、今川正良
  • Organizer: 第9回次世代を担う若手ファーマ・バイオフォーラム 2010
  • Place of Presentation: 京都
  • Year and Date: 2010-10-02
• [Presentation] 脂肪細胞分化過程において Rho GTPase, TCL/TC10・L が RhoA シグナルに与える影響の解析 (2010)
  • Author(s): 大橋健吾、西塚 誠、長田茂宏、今川正良
  • Organizer: 日本薬学会第130 年会
  • Place of Presentation: 岡山
  • Year and Date: 2010-03-29
• [Presentation] 脂肪細胞分化過程における KCNK10 の機能解析 (2010)
  • Author(s): 浅野真美、西塚 誠、佐藤周史、長田茂宏、今川正良
  • Organizer: 日本薬学会第130 年会
  • Place of Presentation: 岡山
  • Year and Date: 2010-03-28
• [Presentation] 脂肪細胞分化の分子機構 (2010)
  • Author(s): 今川正良
  • Organizer: 北陸大学学術フロンティア年次研究集会・総括集会
  • Place of Presentation: 金沢
  • Year and Date: 2010-03-16
• [Presentation] RGS2, TCL/TC10betaL and fad104 promote mitotic clonal expansion at the early stage of adipocyte differentiation. (2009)
  • Author(s): Atsuko Kawaji, Makoto Nishizuka, Shigehiro Osada, Masayoshi Imagawa
  • Organizer: 21st IUBMB and 12th FAOBMB International Congress of Biochemistry and MolecularBiology
  • Place of Presentation: Shanghai, China.
  • Year and Date: 20090802-07
• [Presentation] Ku proteins interact with farnesoid X receptor and function as corepressors. (2009)
  • Author(s): Masae Ohno, Makoto Nishizuka, Shigehiro Osada, Masayoshi Imagawa
  • Organizer: 21st IUBMB and 12th FAOBMB International Congress of Biochemistry and Molecular Biology.
  • Place of Presentation: Shanghai, China.
  • Year and Date: 20090802-07
• [Presentation] Mouse embryonic fibroblasts (MEF) を用いた fad104 の機能解析 (2009)
  • Author(s): 加藤愛友美、岸本圭史、長田茂宏、西塚 誠、今川正良
  • Organizer: 日本病院薬剤師会東海ブロック・日本薬学会東海支部合同学術大会 2009
  • Place of Presentation: 四日市
  • Year and Date: 2009-11-23
• [Presentation] 脂肪細胞分化に重要である fad104 の肺発生における役割 (2009)
  • Author(s): 岸本圭史、西塚 誠、長田茂宏、今川正良
  • Organizer: 第8回次世代を担う若手ファーマ・バイオフォーラム 2009
  • Place of Presentation: 名古屋
  • Year and Date: 2009-11-14
• [Presentation] 脂肪細胞分化に着目した新たな肥満治療薬および肥満予防薬の開発 (2009)
  • Author(s): 今川正良
  • Organizer: 第8回国際バイオフォーラム
  • Place of Presentation: 東京
  • Year and Date: 2009-07-02
• [Remarks] 研究室ホームページ情報
  • URL: http://www.phar.nagoya-cu.ac.jp/hp/byg/index.htm
• [Patent(Industrial Property Rights)] 脂肪細胞分化関連遺伝子およびタンパク質 (2010)
  • Inventor(s): 今川正良、奥裕一
  • Industrial Property Rights Holder: 日水製薬株式会社
  • Industrial Property Number: 第4504682 号
  • Acquisition Date: 2010-04-30
• [Patent(Industrial Property Rights)] Adipocyte differentiationassociated gene and protein (2009)
  • Inventor(s): Imagawa, M., and Oku, Y.
  • Industrial Property Rights Holder: NISSUI PHARMACEUTICAL CO,LTD.
  • Industrial Property Number: EP1 462 518 B1
  • Acquisition Date: 2009-04-08
  • Overseas