2011 Fiscal Year Final Research Report
Cell Biological Analysis of Lipidic Supramolecular Structure
| Project/Area Number |
21390053
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
General anatomy (including Histology/Embryology)
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| Research Institution | Nagoya University |
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Principal Investigator |
FUJIMOTO Toyoshi 名古屋大学, 大学院・医学系研究科, 教授 (50115929)
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| Co-Investigator(Kenkyū-buntansha) |
OHSAKI Yuki 名古屋大学, 大学院・医学系研究科, 助教 (00378027)
SUZUKI Michitaka 名古屋大学, 大学院・医学系研究科, 助教 (80456649)
FUJITA Akikazu 鹿児島大学, 農学部, 教授 (60282232)
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| Project Period (FY) |
2009 – 2011
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| Keywords | 脂肪滴 / 膜ドメイン / 膜脂質 / 凍結割断レプリカ法 / 電子顕微鏡 |
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| Research Abstract |
(1) We found that lipidated ApoB is dislocated from the ER lumen to the lipid droplet surface for proteasomal degradation and that UBXD8 and Derlin-1 are critically involved in the mechanism. Derlin-1 is engaged in the dislocation step, whereas UBXD8 functions after the dislocation. The result elucidated the molecular mechanism of ApoB degradation process that occurs on the lipid droplet surface.
(2) We demonstrated the nanoscale distribution of phosphatidylinositol 4, 5bisphosphate[PI(4, 5) P2] by the quick-freezing, freeze-fracture replica labeling method. The following findings were obtained : in cultured fibroblasts, PI(4, 5) P2 is concentrated at the orifice of caveolae and coated pits and shows different behaviors from that in the flat undifferentiated membrane region ; in rat pancreatic epithelial cells in vivo PI(4, 5) P2 was found densely distributed in the gap junction.
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Research Products
(32 results)
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[Journal Article] Hepatitis C virus infectivity is influenced by association of apolipoprote in Eisoforms2010
Author(s)
Hishiki T, Shimizu Y, Tobita R, Sugiyama K, Ogawa K, Funami K, Ohsaki Y, Fujimoto T, Takaku H, Wakita T, Baumert TF, Miyanari Y, Shimotohno K.
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Journal Title
J Virol
Volume: 84
Pages: 12048-12057
Peer Reviewed
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[Journal Article] A distinct pool of phosphatidylinositol 4, 5-bisphosphate in caveolae revealed by a nanoscale labeling technique2009
Author(s)
Fujita, A., Cheng, J., Tauchi-Sato, K., Takenawa, T., Fujimoto, T
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Journal Title
Proc. Natl. Acad. Sci. USA
Volume: 106
Pages: 9256-9261
Peer Reviewed
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[Journal Article] Quantitative electron microscopy shows uniform incorporation of triglycerides into existing lipid droplets.2009
Author(s)
Cheng, J., Fujita, A., Ohsaki, Y., Suzuki, M., Shinohara, Y., Fujimoto, T.
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Journal Title
Histochem. Cell Biol
Volume: 132
Pages: 281-291
Peer Reviewed
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[Journal Article] Biogenesis of cytoplasmic lipid droplets : from the lipid ester globule in the membrane to the visible structure2009
Author(s)
Ohsaki, Y., Cheng, J., Suzuki, M., Shinohara, Y., Fujita, A., Fujimoto, T.
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Journal Title
Biochim. Biophys. Acta
Volume: 1791
Pages: 399-407
Peer Reviewed
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