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2011 Fiscal Year Final Research Report

Cell Biological Analysis of Lipidic Supramolecular Structure

Research Project

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Project/Area Number 21390053
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field General anatomy (including Histology/Embryology)
Research InstitutionNagoya University

Principal Investigator

FUJIMOTO Toyoshi  名古屋大学, 大学院・医学系研究科, 教授 (50115929)

Co-Investigator(Kenkyū-buntansha) OHSAKI Yuki  名古屋大学, 大学院・医学系研究科, 助教 (00378027)
SUZUKI Michitaka  名古屋大学, 大学院・医学系研究科, 助教 (80456649)
FUJITA Akikazu  鹿児島大学, 農学部, 教授 (60282232)
Project Period (FY) 2009 – 2011
Keywords脂肪滴 / 膜ドメイン / 膜脂質 / 凍結割断レプリカ法 / 電子顕微鏡
Research Abstract

(1) We found that lipidated ApoB is dislocated from the ER lumen to the lipid droplet surface for proteasomal degradation and that UBXD8 and Derlin-1 are critically involved in the mechanism. Derlin-1 is engaged in the dislocation step, whereas UBXD8 functions after the dislocation. The result elucidated the molecular mechanism of ApoB degradation process that occurs on the lipid droplet surface.
(2) We demonstrated the nanoscale distribution of phosphatidylinositol 4, 5bisphosphate[PI(4, 5) P2] by the quick-freezing, freeze-fracture replica labeling method. The following findings were obtained : in cultured fibroblasts, PI(4, 5) P2 is concentrated at the orifice of caveolae and coated pits and shows different behaviors from that in the flat undifferentiated membrane region ; in rat pancreatic epithelial cells in vivo PI(4, 5) P2 was found densely distributed in the gap junction.

  • Research Products

    (32 results)

All 2012 2011 2010 2009 Other

All Journal Article (13 results) (of which Peer Reviewed: 13 results) Presentation (13 results) Book (5 results) Remarks (1 results)

  • [Journal Article] Derlin-1 and UBXD8 are engaged in dislocation and degradation of lipidated ApoB-100 at lipid droplets2012

    • Author(s)
      Suzuki M, Otsuka T, Ohsaki Y, Cheng J, Taniguchi T, Hashimoto H, Taniguchi H, and Fujimoto T.
    • Journal Title

      Mol Biol Cell

    • Peer Reviewed
  • [Journal Article] The distribution of phosphatidylinositol 4, 5-bisphosphate in acinar cells of rat pancreas revealed with the freeze-fracture replica labeling method2011

    • Author(s)
      Sakurai-Ozato N, Fujita A, Fujimoto T.
    • Journal Title

      PLoS ONE

      Volume: 6 Pages: 23567

    • DOI

      DOI:10.1371/journal.pone.0023567

    • Peer Reviewed
  • [Journal Article] Not just fat : the structure and function of the lipid droplet2011

    • Author(s)
      Fujimoto T, Parton RG.
    • Journal Title

      Cold Spring Harbor Perspect Biol

      Volume: 3

    • DOI

      doi:10.1101/cshperspect.a004838,2011

    • Peer Reviewed
  • [Journal Article] Lipid droplets : Size matters2011

    • Author(s)
      uzuki M, Shinohara Y, Ohsaki Y, FujimotoT.
    • Journal Title

      J Electron Microsc

      Volume: 60 Pages: 101-116

    • Peer Reviewed
  • [Journal Article] Quantitative electron microscopy for the nanoscale analysis of membrane lipid distribution2010

    • Author(s)
      Fujita, A., Cheng, J., Fujimoto, T.
    • Journal Title

      Nature Protocols

      Volume: 5 Pages: 661-669

    • Peer Reviewed
  • [Journal Article] A pitfall in using BODIPY dyes to label lipid droplets for fluorescence microscopy2010

    • Author(s)
      Ohsaki, Y., Shinohara, Y., Suzuki, M., Fujimoto, T.
    • Journal Title

      Histochem. Cell Biol

      Volume: 133 Pages: 477-480

    • Peer Reviewed
  • [Journal Article] Lipoprotein lipase and hepatic triglyceride lipase reduce the infectivity of hepatitis C virus(HCV) through their catalytic activities on HCV-associate dlipoproteins2010

    • Author(s)
      Shimizu Y, Hishiki T, Sugiyama K, OgawaK, Funami K, Kato A, Ohsaki Y, Fujimoto T, Takaku H, Shimotohno K.
    • Journal Title

      Virology

      Volume: 407 Pages: 152-159

    • Peer Reviewed
  • [Journal Article] Hepatitis C virus infectivity is influenced by association of apolipoprote in Eisoforms2010

    • Author(s)
      Hishiki T, Shimizu Y, Tobita R, Sugiyama K, Ogawa K, Funami K, Ohsaki Y, Fujimoto T, Takaku H, Wakita T, Baumert TF, Miyanari Y, Shimotohno K.
    • Journal Title

      J Virol

      Volume: 84 Pages: 12048-12057

    • Peer Reviewed
  • [Journal Article] Lysosomal accumulation of mTOR is enhanced by rapamycin2010

    • Author(s)
      Ohsaki Y, Suzuki M, Shinohara Y, Fujimoto T.
    • Journal Title

      Histochem Cell Biol

      Volume: 134 Pages: 537-544

    • Peer Reviewed
  • [Journal Article] A distinct pool of phosphatidylinositol 4, 5-bisphosphate in caveolae revealed by a nanoscale labeling technique2009

    • Author(s)
      Fujita, A., Cheng, J., Tauchi-Sato, K., Takenawa, T., Fujimoto, T
    • Journal Title

      Proc. Natl. Acad. Sci. USA

      Volume: 106 Pages: 9256-9261

    • Peer Reviewed
  • [Journal Article] Segregation of GM1 and GM3 clusters in the cell membrane depends on the intact actin cytoskeleton2009

    • Author(s)
      Fujita, A., Cheng, J., Fujimoto, T.
    • Journal Title

      Biochim. Biophys. Acta

      Volume: 1791 Pages: 388-396

    • Peer Reviewed
  • [Journal Article] Quantitative electron microscopy shows uniform incorporation of triglycerides into existing lipid droplets.2009

    • Author(s)
      Cheng, J., Fujita, A., Ohsaki, Y., Suzuki, M., Shinohara, Y., Fujimoto, T.
    • Journal Title

      Histochem. Cell Biol

      Volume: 132 Pages: 281-291

    • Peer Reviewed
  • [Journal Article] Biogenesis of cytoplasmic lipid droplets : from the lipid ester globule in the membrane to the visible structure2009

    • Author(s)
      Ohsaki, Y., Cheng, J., Suzuki, M., Shinohara, Y., Fujita, A., Fujimoto, T.
    • Journal Title

      Biochim. Biophys. Acta

      Volume: 1791 Pages: 399-407

    • Peer Reviewed
  • [Presentation] Nanoscale detection of membrane lipids by freeze-fracture electron microscopy2011

    • Author(s)
      Toyoshi Fujimoto
    • Organizer
      University of Washington-Kobe University Joint Symposium on Integrative Membrane Biology and Signal Transduction Medicine
    • Place of Presentation
      Kobe
    • Year and Date
      20111213-14
  • [Presentation] Nanoscale distribution of phospholipids observed by electron microscopy2011

    • Author(s)
      Toyoshi Fujimoto
    • Organizer
      第9回JBSバイオフロンティア国際シンポジウム
    • Place of Presentation
      福岡
    • Year and Date
      20111114-16
  • [Presentation] 電顕で脂質を見る2011

    • Author(s)
      藤本豊士
    • Organizer
      第52回日本組織細胞化学会総会・学術集会
    • Place of Presentation
      金沢
    • Year and Date
      20110923-24
  • [Presentation] 膜脂質分布のナノレベル局在解析2011

    • Author(s)
      藤本豊士
    • Organizer
      第84回日本生化学会大会
    • Place of Presentation
      京都
    • Year and Date
      20110921-24
  • [Presentation] Lipid droplets as aplatform of protein degradation2011

    • Author(s)
      Toyoshi Fujimoto
    • Organizer
      The 30th NaitoConference on Membrane Dynamics and Lipid Biology[II]
    • Place of Presentation
      Sapporo
    • Year and Date
      20110628-0701
  • [Presentation] Nanoscale detection of membrane lipids byelectron microscopy2011

    • Author(s)
      藤本豊士、藤田秋一、櫻井納美、程晶磊
    • Organizer
      第67回日本顕微鏡学会学術講演会
    • Place of Presentation
      福岡
    • Year and Date
      20110516-18
  • [Presentation] Nanoscale detection of membrane lipids by electron microscopy2011

    • Author(s)
      Toyoshi Fujimoto
    • Organizer
      The 2nd International Symposium on Membrane Biology
    • Place of Presentation
      Ningbo, China
    • Year and Date
      2011-10-12
  • [Presentation] Quantitative electron microscopy to observe nanoscale distribution of membrane2010

    • Author(s)
      Toyoshi Fujimoto
    • Organizer
      The 13th Membrane Forum
    • Place of Presentation
      Kyoto
    • Year and Date
      20101206-28
  • [Presentation] Nanoscale detection of membranelipids by electron microscopy2010

    • Author(s)
      Akikazu Fujita, Jinglei Cheng, and Toyoshi Fujimoto
    • Organizer
      The 4th International Society for Neurochemistry Special Neurochemistry Conference"Membrane domainsin CNS physiology and pathology"
    • Place of Presentation
      Erice, Italy
    • Year and Date
      20100522-25
  • [Presentation] 凍結割断レプリカ標識法によるイノシトール燐脂質局在の解析2009

    • Author(s)
      藤本豊士,藤田秋一,程晶磊
    • Organizer
      第82回日本生化学会大会
    • Place of Presentation
      神戸
    • Year and Date
      20091021-24
  • [Presentation] 脂肪滴の構造と機能2009

    • Author(s)
      藤本豊士
    • Organizer
      第50回日本組織細胞化学会総会・学術集会
    • Place of Presentation
      大津
    • Year and Date
      20090926-28
  • [Presentation] Nanoscale localization of membrane lipids by electron microscopy2009

    • Author(s)
      Toyoshi Fujimoto
    • Organizer
      The 4^<th> iCeMS International Symposium
    • Place of Presentation
      Kyoto
    • Year and Date
      20090527-29
  • [Presentation] 膜ドメインと膜脂質局在2009

    • Author(s)
      藤本豊士
    • Organizer
      第25回Wakoワークショップ「細胞膜ナノドメイン:統合的理解と新たな展開
    • Place of Presentation
      東京
    • Year and Date
      2009-11-17
  • [Book] 膜を構成する分子の解析法、日本組織細胞化学会編「組織細胞化学2011」2011

    • Author(s)
      藤本豊士、鈴木倫毅、櫻井納美、藤田秋一
    • Total Pages
      55-61
  • [Book] High ResolutionMolecular Localization by Freeze-FractureReplica Labeling, Schwartzbach, S. D., Osafune, T.(eds.)Immunoelectron Microscopy : Methodsand Protocols2010

    • Author(s)
      Fujita, A., Fujimoto, T
    • Total Pages
      205-216
    • Publisher
      Humana Press
  • [Book] 脂質・脂肪滴の光顕・電顕検出法、日本組織細胞化学会編「組織細胞化学2010」2010

    • Author(s)
      大崎雄樹, 篠原友樹, 鈴木倫毅, 藤本豊士
    • Total Pages
      39-45
  • [Book] 見えてきた膜ドメインの姿と生体脂質のダイナミクス実験医学2010

    • Author(s)
      池ノ内順一、藤本豊士(編集)
    • Total Pages
      28
  • [Book] 脂質の組織化学日本組織細胞化学会「組織細胞化学20099」2009

    • Author(s)
      藤田秋一, 大崎雄樹, 藤本豊士
    • Total Pages
      35-44
  • [Remarks]

    • URL

      http://www.med.nagoya-u.ac.jp/cel-bio

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Published: 2013-07-31  

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