An interrelation among regulatory mechanisms of cardiac Ca2+channels

2011 Fiscal Year Final Research Report

• Project/Area Number: 21390059
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: General physiology
• Research Institution: Kagoshima University
• Principal Investigator: KAMEYAMA Masaki 鹿児島大学, 医歯学総合研究科, 教授 (60150059)
• Co-Investigator(Kenkyū-buntansha): YAZAWA Kazuto 鹿児島大学, 大学院・医歯学総合研究科, 講師 (90212274) ; MINOBE Etsuko 鹿児島大学, 大学院・医歯学総合研究科, 助教 (00448581)
• Co-Investigator(Renkei-kenkyūsha): XU Jian-jun 鹿児島大学, 大学院・医歯学総合研究科, 助教 (10581689)
• Research Collaborator: KAMEYAMA Asako 鹿児島大学, 大学院・医歯学総合研究科, 客員研究員
• Project Period (FY): 2009 – 2011
• Keywords: 生体膜 / チャネル / シグナル伝達

Research Abstract

We investigated regulation of L-type Ca2+channels by calmodulin(CaM) and phosphorylation. CaM bound to preIQ and IQ regions in the C-terminal of the channel. PKA phosphorylated Ser1574 and Ser1626 as well as Ser1927(guinea-pig). The facilitatory effect of CaM was enhanced by CaMKII-mediated phosphorylation of Thr1603, suggesting a cross-talk between the CaM's effect and channel phosphorylation.

Research Products

• [Journal Article] Calpastatin domain L is a partial agonist of the calmodulin-binding site for channel activation in Cav1 (2011)
  • Author(s): Minobe E, Asmara H, Saud ZA, and Kameyama M.
  • Journal Title: J Biol Chem Volume: 286(45) Pages: 39013-39022
  • Peer Reviewed
• [Journal Article] Interactions of calmodulin with the multiple binding sites of Cav1. 2 Ca^<2+> channels (2010)
  • Author(s): Asmara H, Minobe E, Saud ZA, Kameyama M.
  • Journal Title: J Pharmacol Sci Volume: 112(4) Pages: 397-404
  • Peer Reviewed
• [Journal Article] Calmodulin-and Ca^<2+>-dependent facilitation and inactivation of the CaV1. 2 Ca^<2+> channels in guinea-pig ventricular myocytes (2010)
  • Author(s): Han DY, Minobe E, Wang WY, Guo F, Xu JJ, Hao LY, Kameyama M.
  • Journal Title: J Pharmacol Sci Volume: 112(3) Pages: 310-319
  • Peer Reviewed
• [Journal Article] Both N-and C-lobes of calmodulin are required for Ca^<2+>-dependent regulations of CaV1. 2 Ca^<2+> channels (2010)
  • Author(s): Guo F, Minobe E, Yazawa K, Asmara H, Bai XY, Han DY, Hao LY and Kameyama M.
  • Journal Title: Biochem Biophys Res Comm Volume: 391(2) Pages: 1170-1176
  • Peer Reviewed
• [Journal Article] The distinct roles of calmodulin and calmodulin kinase II in the reversal of run-down of L-type Ca^<2+> channels in guinea-pig ventricular myocytes (2009)
  • Author(s): Hao LY, Wang WY, Minobe M, Han DY, Xu JJ, Kameyama A, Kameyama M.
  • Journal Title: J. Pharmacol. Sci Volume: 111(4) Pages: 416-425
  • Peer Reviewed
• [Journal Article] CaMKII phosphorylates a threonine residue in the C-terminal tail of Cav1. 2 Ca^<2+> channel and modulates the interaction of the channel with calmodulin (2009)
  • Author(s): Wang WY, Hao LY, Minobe E, Saud ZA, Han DY, Kameyama M.
  • Journal Title: J Physiol Sci Volume: 59(4) Pages: 283-90
  • Peer Reviewed
• [Presentation] モルモット心室筋細胞におけるL型Ca^<2+>チャネルの過酸化水素による活性調節機構 (2012)
  • Author(s): 楊磊、徐建軍、蓑部悦子、封瑞、亀山亜砂子、矢沢和人、亀山正樹
  • Organizer: 第89回日本生理学会大会
  • Place of Presentation: 松本
  • Year and Date: 20120329-31
• [Presentation] Cav1. 2 Ca^<2+>チャネルC末端部には、複数の機能的Aキナーゼリン酸化部位がある (2012)
  • Author(s): 蓑部悦子、前田佐知子、徐建軍、はお麗英、封瑞、矢沢和人、亀山亜砂子、亀山正樹
  • Organizer: 第89回日本生理学会大会
  • Place of Presentation: 松本
  • Year and Date: 20120329-31
• [Presentation] Cav1. 2 Ca^<2+>チャネルの活性調節におけるカルパスタチンとカルモジュリンの競合作用 (2012)
  • Author(s): 蓑部悦子、Hadhimulya Asmara, Zahangir A. Saud, 亀山正樹
  • Organizer: 第89回日本生理学会大会
  • Place of Presentation: 松本
  • Year and Date: 20120329-31
• [Presentation] CaMKII-dependent and independent facilitation of L-type Ca^<2+> channels by hydrogen peroxide in guinea-pig ventricular myocytes (2011)
  • Author(s): Yang L, Xu J-J, Minobe E, Feng R, Kameyama A, Yazawa K, Kameyama M.
  • Organizer: The 62th meeting of the Physiological Society of West Japan
  • Place of Presentation: Saga
  • Year and Date: 20111014-15
• [Presentation] Facilitation of L-type Ca^<2+> channels by hydrogen peroxide in guinea-pig ventricular myocytes (2011)
  • Author(s): Yang L, Xu J-J, Minobe E, Feng R, Kameyama A, Yazawa K, Kameyama M.
  • Organizer: The 5th Biennial Meeting of Society for Free Radical Research(SFRR)-Asia and The 8th Conference of Asian Society for Mitochondrial Research and Medicine(ASMRM)
  • Place of Presentation: Kagoshima
  • Year and Date: 20110831-0904
• [Presentation] Effect of Ca^<2+> and calmodulin on activity of calmodulin-linked Cav1. 2 channel (2011)
  • Author(s): Minobe E, Mori MX, Kameyama M.
  • Organizer: The 88th Annual Meeting of the Physiological Society of Japan
  • Year and Date: 20110300
• [Presentation] Both PKA-mediated phosphorylation and calmodulin interaction are required for basal activity of cardiac L-type Ca^<2+> channel (2011)
  • Author(s): Xu J-J, Saud ZA, Liu Y, Yang L, Minobe E, Kameyama A, Yazawa K, Hao L-Y, Kameyama M.
  • Organizer: The 88th Annual Meeting of the Physiological Society of Japan
  • Year and Date: 20110300
• [Presentation] PKA-mediated phosphorylation modulates the interaction of calmod ulin with cardiac Cav1. 2 channels (2010)
  • Author(s): Xu J-J, Saud ZA, Liu Y, Yang L, Minobe E, Kameyama A, Yazawa K, Hao L-Y, Kameyama M.
  • Organizer: The 9th Japan-Korea Joint Symposium on Brain Sciences, and Cardiac and Smooth Muscles
  • Place of Presentation: Kagoshima
  • Year and Date: 20101125-28
• [Presentation] Ca^<2+>-dependent modulation in calmodulin-linked Cav1. 2 channels (2010)
  • Author(s): Minobe E, Mori MX, Xu J-J, Kameyama M.
  • Organizer: The 9th Japan-Korea Joint Symposium on Brain Sciences, and Cardiac and Smooth Muscles
  • Place of Presentation: Kagoshima
  • Year and Date: 20101125-28
• [Presentation] Roles of calmodulin and the C-terminal tail of Cav1. 2 channel in its activity (2010)
  • Author(s): Minobe E, Mori MX, Kameyama M.
  • Organizer: 第87回日本生理学会大会
  • Place of Presentation: 盛岡
  • Year and Date: 20100519-21
• [Presentation] Multiple molecules of calmodulin bind to the Cav1. 2 Ca^<2+> channel (2009)
  • Author(s): Asmara H, Minobe E, Saud ZA, Kameyama M.
  • Organizer: The 60th meeting of the Physiological Society of West Japan
  • Place of Presentation: Fukuoka
  • Year and Date: 20091106-07
• [Presentation] Regulation of Cav1. 2 Ca^<2+> channels by Ca^<2+>, calmodulin and CaMKII (2009)
  • Author(s): Kameyama M, Hao L-Y, Minobe E, Xu J-J, Han D-Y, Nie H-G, Saud ZA, Wang W-Y, Asmara H, Yazawa K.
  • Organizer: IUPS Satellite Symposium on Ion channels : function, structure and physiology
  • Place of Presentation: Hiei/Kyoto
  • Year and Date: 20090802-04
• [Presentation] Multiple binding sites of calmodulin in the C-terminal tail of Cav1. 2 Ca^<2+> channel (2009)
  • Author(s): Asmara H, Minobe E, Saud ZA, Wang W-Y, Kameyama M.
  • Organizer: The 36th International Congress of Physiological Sciences(IUPS2009)
  • Place of Presentation: Kyoto
  • Year and Date: 20090727-0801
• [Presentation] Facilitatory and inhibitory effects of calmodulin on activity of Cav1. 2 Ca^<2+> channel (2009)
  • Author(s): Han D-Y, Hao L-Y, Minobe E, Wang W-Y, Xu J-J, Kamey ama M.
  • Organizer: The 36th International Congress of Physiological Sciences(IUPS2009)
  • Place of Presentation: Kyoto
  • Year and Date: 20090727-0801
• [Presentation] Calpastatin domain L competes with calmodulin on IQ region of Cav1. 2 channel (2009)
  • Author(s): Minobe E, Saud ZA, Han D-Y, Asmara H, Wang W-Y, Hao L-Y, Kameyama M.
  • Organizer: The 36th International Congress of Physiological Sciences(IUPS2009)
  • Place of Presentation: Kyoto
  • Year and Date: 20090727-0801
• [Presentation] Ca^<2+> channels and cardiac functions (2009)
  • Author(s): Kameyama M, Hao L-Y, Minobe E, Xu J-J, Han D-Y, Nie H-G, Saud ZA, Wang W-Y, Asmara H.
  • Organizer: The 36th International Congress of Physiological Sciences(IUPS2009)
  • Place of Presentation: Kyoto
  • Year and Date: 20090727-0801
• [Remarks]
  • URL: URL:http://www.kufm.kagoshima-u.ac.jp/～physiol2/