2011 Fiscal Year Final Research Report
Regulation of aquaporin-4 by formation of protein complex and orthogonal array
| Project/Area Number |
21390061
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
General physiology
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| Research Institution | Keio University |
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Principal Investigator |
YASUI Masato 慶應義塾大学, 医学部, 教授 (90246637)
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| Co-Investigator(Kenkyū-buntansha) |
NAKAO Kazuki 独立行政法人理化学研究所, 動物実験支援ユニット, ユニットリーダー (20217657)
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| Co-Investigator(Renkei-kenkyūsha) |
ABE Yoichiro 慶應義塾大学, 医学部, 助教 (10317331)
YUKUTAKE Yoshinori 慶應義塾大学, 医学部, 助教 (80449016)
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| Project Period (FY) |
2009 – 2011
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| Keywords | アクアポリン / 蛋白複合体 / 構造機能相関 / 水分子 / 脳浮腫 |
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| Research Abstract |
To investigate physiological roles of square array formation of aquaporin-4 (AQP4) in vivo, we established two kinds of AQP4 knockout mice: M1 isoform-specific KO, which is expected to possess much larger arrays than wild type, and null mice. As a result of comprehensive phenotype analysis, we found that appropriate size of the square arrays is essential for the retinal response to light stimulation. In addition, we demonstrated that AQP4 plays an important role for immunological function in the central nervous system by making stub-wounded model using AQP4-null mice. Both types of knockout mice have been deposited at RIKEN BRC so as to be used freely by other researchers.
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[Journal Article] Establishment of Monoclonal antibodies against the extracellular domain that block binding of NMO-IgG to AQP42013
Author(s)
Miyazaki K, Abe Y, Iwanari H, Suzuki Y, Kikuchi T, Ito T, Kato J, Kusano-Arai O, Takahashi T, Nishiyama S, Ikeshima-Kataoka H, Tsuji S, Arimitsu T, Kato Y, Sakihama T, Toyama Y, Fujihara K, Hamakubo T and Yasui M
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Journal Title
J. Neuroimmunol
Volume: (in press)
Peer Reviewed
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