Visulization of Wnt/Notch signaling for the maintenance of mesenchymal stem cells

2011 Fiscal Year Final Research Report

• Project/Area Number: 21390062
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: General physiology
• Research Institution: Keio University
• Principal Investigator: MATSUZAKI Yumi 慶應義塾大学, 医学部, 特任准教授 (50338183)
• Co-Investigator(Kenkyū-buntansha): KAWAMURA Yoshimi 慶應義塾大学, 医学部, 助教 (20505044) ; SUZUKI Sadafumi 慶應義塾大学, 医学部, 研究員 (70465003) ; MORIKAWA Satoru 慶應義塾大学, 医学部, 助教 (00424169) ; MABUCHI Yo 慶應義塾大学, 医学部, 助教 (50424172)
• Project Period (FY): 2009 – 2011
• Keywords: 間葉系幹細胞 / 再生医学 / 細胞・組織 / 移植・再生医療

Research Abstract

It has been advocated that Wnt, Notch signals involve molecular mechanism of self-renewal or differentiation in various somatic stem cells. In mammalian development, stem cell controls complicated organizational structure as high-order function, while controlling self-renew and differentiation. In this project, we attempted to clarify self-renewal and cell lineage decision mechanism of MSC. Our method was to visualize Wnt, Notch signals, which has a potential for temporospatial controls of stem cells. By visualizing Wnt, Notch signals, we could analyze how Wnt/Notch separates their roles, or interacts with other molecules in order for self-replication.

Research Products

• [Journal Article] Neural Stem Cells Directly Differentiated from Partially Reprogrammed Fibroblasts Rapidly Acquire Gliogenic Competency (2012)
  • Author(s): Matsui T, Takano M, Yoshida K, Ono S, Fujisaki C, Matsuzaki Y, Toyama Y, Nakamura M, Okano H, Akamatsu W
  • Journal Title: Stem Cells
  • DOI: doi:10.1002/stem.1091
  • Peer Reviewed
• [Journal Article] RNA-Binding Protein Musashi1 Modulates Glioma Cell Growth through the Post-Transcriptional Regulation of Notch and PI(3) Kinase/Akt Signaling Pathways (2012)
  • Author(s): Muto J, Imai T, Ogawa D, Nishimoto Y, Okada Y, Mabuchi Y, Kawase T, Iwanami A, Mischel PS, Saya H, Yoshida K, Matsuzaki Y, Okano H
  • Journal Title: PLoS One Volume: 7(3)
  • DOI: doi:10.1371/journal.pone.0033431
  • Peer Reviewed
• [Journal Article] Bioluminescent system for dynamic imaging of cell and animal behavior (2012)
  • Author(s): Hara-Miyauchi C, Tsuji O, Hanyu A, Okada S, Yasuda A, Fukano T, Akazawa C, Nakamura M, Imamura T, Matsuzaki Y, Okano HJ, Miyawaki A, Okano H
  • Journal Title: Biochem Biophys Res Commun Volume: 419(2) Pages: 188-93
  • DOI: doi:10.1016/j.bbrc.2012.01.141
  • Peer Reviewed
• [Journal Article] Generation of stratified squamous epithelial progenitor cells from mouse induced pluripotent stem cells (2011)
  • Author(s): Yoshida S, Yasuda M, Miyashita H, Ogawa Y, Yoshida T, Matsuzaki Y, Tsubota K, Okano H, Shimmura S
  • Journal Title: PLoS One Volume: 6(12) Pages: 1
  • DOI: doi:10.1371/journal.pone.0028856
  • Peer Reviewed
• [Journal Article] The dual origin of the peripheral olfactory system : placode and neural crest (2011)
  • Author(s): Katoh H, Shibata S, Fukuda K, Sato M, Satoh E, Nagoshi N, Minematsu T, Matsuzaki Y, Akazawa C, Toyama Y, Nakamura M, Okano H
  • Journal Title: Mol Brain Volume: 4 Pages: 34
  • DOI: doi:10.1186/1756-6606-4-34
  • Peer Reviewed
• [Journal Article] Functional analysis of HOXD9 in human gliomas and glioma cancer stem cells (2011)
  • Author(s): Tabuse M, Ohta S, Ohashi Y, Fukaya R, Misawa A, Yoshida K, Kawase T, Saya H, Thirant C, Chneiweiss H, Matsuzaki Y, Okano H, Kawakami Y, Toda M
  • Journal Title: Mol Cancer Volume: 10 Pages: 60
  • DOI: doi:10.1186/1476-4598-10-60
  • Peer Reviewed
• [Journal Article] Identification of a Novel Intronic Enhancer Responsible for the Transcriptional Regulation of Musashi1 in Neural Stem/Progenitor Cells (2011)
  • Author(s): Kawase S, Imai T, Miyauchi-Hara C, Yaguchi K, Nishimoto Y, Fukami SI, Matsuzaki Y, Miyawaki A, Itohara S, Okano H
  • Journal Title: Mol Brain Volume: 4(1) Pages: 14
  • DOI: doi:10.1186/1756-6606-4-14
  • Peer Reviewed
• [Journal Article] Purified Mesenchymal Stem Cells Are an Efficient Source for iPS Cell Induction (2011)
  • Author(s): Niibe K, Kawamura Y, Araki D, Morikawa S, Miura K, Suzuki S, Shimmura S, Sunabori T, Mabuchi Y, Nagai Y, Nakagawa T, Okano H, Matsuzaki Y
  • Journal Title: PLoS One Volume: 6(3)
  • DOI: doi:10.1371/journal.pone.0017610
  • Peer Reviewed
• [Journal Article] Schwann cell plasticity after spinal cord injury shown by neural crest lineage tracing (2011)
  • Author(s): Nagoshi N, Shibata S, Hamanoue M, Mabuchi Y, Matsuzaki Y, Toyama Y, Nakamura M, Okano H
  • Journal Title: Glia
  • DOI: doi:10.1002/glia.21150
  • Peer Reviewed
• [Journal Article] Generation of human melanocytes from induced pluripotent stem cells (2011)
  • Author(s): Ohta S, Imaizumi Y, Okada Y, Akamatsu W, Kuwahara R, Ohyama M, Amagai M, Matsuzaki Y, Yamanaka S, Okano H, Kawakami Y
  • Journal Title: PLoS One Volume: 6(1)
  • DOI: doi:10.1371/journal.pone.0016182
  • Peer Reviewed
• [Journal Article] Therapeutic potential of appropriately evaluated safe-induced pluripotent stem cells for spinal cord injury (2010)
  • Author(s): Tsuji O, Miura K, Okada Y, Fujiyoshi K, Mukaino M, Nagoshi N, Kitamura K, Kumagai G, Nishino M, Tomisato S, Higashi H, Nagai T, Katoh H, Kohda K, Matsuzaki Y, Yuzaki M, Ikeda E, Toyama Y, Nakamura M, Yamanaka S, Okano H
  • Journal Title: Proc Natl Acad Sci U S A Volume: 107(28) Pages: 12704-9
  • DOI: doi:10.1073/pnas.0910106107
  • Peer Reviewed
• [Journal Article] Anti-IL-6-receptor antibody promotes repair of spinal cord injury by inducing microglia-dominant inflammation (2010)
  • Author(s): Mukaino M, Nakamura M, Yamada O, Okada S, Morikawa S, Renault-Mihara F, Iwanami A, Ikegami T, Ohsugi Y, TsujiO, Katoh H, Matsuzaki Y, Toyama Y, Liu M, Okano H
  • Journal Title: Exp Neurol Volume: 224(2) Pages: 403-14
  • DOI: doi:10.1016/j.expneurol.2010.04.020
  • Peer Reviewed
• [Journal Article] Stem cell-like properties of the endometrial side population : implication in endometrial regeneration (2010)
  • Author(s): Masuda H, Matsuzaki Y, Hiratsu E, Ono M, Nagashima T, Kajitani T, Arase T, Oda H, Uchida H, Asada H, Ito M, Yoshimura Y, Maruyama T, Okano H
  • Journal Title: PLoS One Volume: 5(4)
  • DOI: doi:10.1371/journal.pone.0010387
  • Peer Reviewed
• [Journal Article] Inhibition of Abcg2 transporter on primitive hematopoietic stem cells by All-trans retinoic acid increases sensitivity to anthracycline (2010)
  • Author(s): Lein L, Nagai Y, Mabuchi Y, Suzuki S, Morikawa S, Matsuzaki Y
  • Journal Title: Inflammation and Regeneration Volume: 32(2) Pages: 722-31
  • Peer Reviewed
• [Journal Article] Stromal cell-secreted factors promote the survival of embryonic stem cell-derived early neural stem/progenitor cells via the activation of MAPK and PI3K-Akt pathways (2010)
  • Author(s): Ishii S, Okada Y, Kadoya T, Matsuzaki Y, Shimazaki T, Okano H
  • Journal Title: J Neurosci Res Volume: 88(4) Pages: 722-34
  • DOI: doi:10.1002/jnr.22250
  • Peer Reviewed
• [Journal Article] Neovascularization promoted by mononuclear cell transplantation after transient cerebral ischemia in mice (2009)
  • Author(s): Funabiki T, Namiki J, Suzuki S, Matsuzaki Y, Aikawa N
  • Journal Title: Inflammation and Regeneration Volume: 29(1) Pages: 66-72
  • Peer Reviewed
• [Journal Article] Tumor initiating potential of side population cells in human gastric cancer (2009)
  • Author(s): Fukuda K, Saikawa Y, Ohashi M, Kumagai K, Kitajima M, Okano H, Matsuzaki Y, Kitagawa Y
  • Journal Title: Int J Oncol Volume: 34(5) Pages: 1201-7
  • DOI: doi:10.3892/ijo_00000248
  • Peer Reviewed
• [Journal Article] Transplantation of dendritic cells promotes functional recovery from spinal cord injury in common marmoset (2009)
  • Author(s): Yaguchi M, Tabuse M, Ohta S, Ohkusu-Tsukada K, Takeuchi T, Yamane J, Katoh H, Nakamura M, Matsuzaki Y, Yamada M, Itoh T, Nomura T, Toyama Y, Okano H, Toda M
  • Journal Title: Neurosci Res Volume: 65(4) Pages: 384-92
  • DOI: doi:10.1016/j.neures.2009.08.016
  • Peer Reviewed
• [Journal Article] Side population of pancreatic cancer cells predominates in TGF-beta-mediated epithelial to mesenchymal transition and invasion (2009)
  • Author(s): Kabashima A, Higuchi H, Takaishi H, Matsuzaki Y, Suzuki S, Izumiya M, Iizuka H, Sakai G, Hozawa S, Azuma T, Hibi T
  • Journal Title: Int J Cancer Volume: 124(12) Pages: 2771-9
  • DOI: doi:10.1002/ijc.24349
  • Peer Reviewed
• [Journal Article] Roles of ES cell-derived gliogenic neural stem/progenitor cells in functional recovery after spinal cord injury (2009)
  • Author(s): Kumagai G, Okada Y, Yamane J, Nagoshi N, Kitamura K, Mukaino M, Tsuji O, Fujiyoshi K, Katoh H, Okada S, Shibata S, Matsuzaki Y, Toh S, Toyama Y, Nakamura M, Okano H
  • Journal Title: PLoS One Volume: 4(11)
  • DOI: doi:10.1371/journal.pone.0007706
  • Peer Reviewed
• [Journal Article] Prospective isolation and identification of human mesenchymal stem cells by flow cytometry (2009)
  • Author(s): Mabuchi Y, Morikawa S, Suzuki S, Sunabori T, Okano H, and Matsuzaki Y
  • Journal Title: Inflammation and Regeneration Volume: 29(1) Pages: 3-78
  • Peer Reviewed
• [Journal Article] Prospective identification, isolation, and systemic transplantation of multipotent mesenchymal stem cells in murine bone marrow (2009)
  • Author(s): Morikawa S, Mabuchi Y, Kubota Y, Nagai Y, Niibe K, Hiratsu E, Suzuki S, Miyauchi-Hara C, Nagoshi N, Sunabori T, Shimmura S, Miyawaki A, Nakagawa T, Suda T, Okano H, Matsuzaki Y
  • Journal Title: J Exp Med Volume: 206(11) Pages: 2483-96
  • DOI: doi:10.1084/jem.20091046
  • Peer Reviewed
• [Journal Article] Neural crest-derived stem cells display a wide variety of characteristics (2009)
  • Author(s): Nagoshi N, Shibata S, Nakamura M, Matsuzaki Y, Toyama Y, Okano H
  • Journal Title: J Cell Biochem Volume: 107(6) Pages: 1046-52
  • DOI: doi:10.1002/jcb.22213
  • Peer Reviewed
• [Journal Article] Evaluation of human fetal neural stem/progenitor cells as a source for cell replacement therapy for neurological disorders : Properties and tumorigenicity after long-term in vitro maintenance (2009)
  • Author(s): Ogawa D, Okada Y, Nakamura M, Kanemura Y, Okano HJ, Matsuzaki Y, Shimazaki T, Ito M, Ikeda E, Tamiya T, Nagao S, Okano H
  • Journal Title: J Neurosci Res Volume: 87(2) Pages: 307-317
  • DOI: doi:10.1002/jnr.21843
  • Peer Reviewed
• [Journal Article] Development of mesenchymal stem cells partially originate from the neural crest (2009)
  • Author(s): Morikawa S, Mabuchi Y, Niibe K, Suzuki S, Nagoshi N, Sunabori T, Shimmura S, Nagai Y, Nakagawa T, Okano H, Matsuzaki Y
  • Journal Title: BBRC Volume: 379(4) Pages: 1114-9
  • DOI: doi:10.1016/j.bbrc.2009.01.031
  • Peer Reviewed
• [Journal Article] Cell surface N-glycans mediated isolation of mouse neural stem cells (2009)
  • Author(s): Hamanoue M, Matsuzaki Y, Sato K, Okano HJ, Shibata S, Sato I, Suzuki S, Ogawara M, Takamatsu K, Okano H
  • Journal Title: J Neurochem Volume: 110(5) Pages: 1575-84
  • DOI: doi:10.1111/j.1471-4159.2009.06256.x
  • Peer Reviewed
• [Journal Article] Bidirectional signaling through ephrinA2-EphA2 enhances osteoclastogenesis and suppresses osteoblastogenesis (2009)
  • Author(s): Irie N, Takada Y, Watanabe Y, Matsuzaki Y, Naruse C, Asano M, Iwakura Y, Suda T, Matsuo K
  • Journal Title: J Biol Chem Volume: 284(21) Pages: 14637-44
  • DOI: doi:10.1074/jbc.M807598200
  • Peer Reviewed
• [Presentation] フローサイトメトリーを用いた間葉計幹細胞の直接分離 (2012)
  • Author(s): 松崎有未
  • Organizer: 次世代医学セミナー・ワークショップ
  • Place of Presentation: 福島県福島県立医科大学
  • Year and Date: 2012-02-29
• [Presentation] フローサイトメトリーによる高純度間葉系幹細胞の分離 (2011)
  • Author(s): 松崎有未
  • Organizer: 第40回日本免疫学会学術集会
  • Place of Presentation: 千葉県幕張メッセ
  • Year and Date: 2011-11-28
• [Presentation] SP細胞の分離 (2011)
  • Author(s): 松崎有未
  • Organizer: 第28回ベックマンコールターセミナー
  • Place of Presentation: 東京都慶應義塾大学医学部信濃町キャンパス
  • Year and Date: 2011-08-18
• [Presentation] フローサイトメトリーによるSP細胞の同定と分離技法 (2011)
  • Author(s): 松崎有未
  • Organizer: ベックマンコールターセミナーIn-House Seminar : SP細胞-解析とソーティング
  • Place of Presentation: カナダMetro Tronto Convention Centre
  • Year and Date: 2011-08-05
• [Presentation] Mesenchymal stem cells & cGVHD (2011)
  • Author(s): 松崎有未
  • Organizer: RIKEN RCAI SEMINAR
  • Place of Presentation: 神奈川県理研鶴見研究所
  • Year and Date: 2011-08-02
• [Presentation] 幹細胞研究の骨軟部腫瘍学への応用:間葉系幹細胞の特徴と機能 (2011)
  • Author(s): 松崎有未
  • Organizer: 第26回日本整形外科学会
  • Place of Presentation: 京都府国立京都国際会館
  • Year and Date: 2011-07-14
• [Presentation] Single Cell Isolation elucidates Heterogeneity within the Human Mesenchymal Stem/Progenitor Cell Compartment(ポスター) (2011)
  • Author(s): 馬渕洋
  • Organizer: ISSCR
  • Place of Presentation: カナダMetro Tronto Convention Centre
  • Year and Date: 2011-06-17
• [Presentation] Hematopoietic and MNSc. Donor MSCs trigger chronic gaft-versushost disease following minor antigen-mismatched bone marrow transplantation (2011)
  • Author(s): 松崎有未
  • Organizer: 第32回日本炎症再生医学会
  • Place of Presentation: 京都府国立京都国際会館
  • Year and Date: 2011-06-03
• [Presentation] Identification and isolation of pure mesenchymal stem cells by flowcytometry (2011)
  • Author(s): 松崎有未
  • Organizer: 熊本大学GCOE特別講義
  • Place of Presentation: 熊本県熊本大学
  • Year and Date: 2011-02-23
• [Presentation] Purified Mesenchymal Stem Cells : An Efficient Cell Source for the iPS Cells Induction (2010)
  • Author(s): 新部邦透
  • Organizer: 8th Annuary Meeting ISSCR
  • Place of Presentation: アメリカMoscone West San Francisco, CA
  • Year and Date: 20100618-19
• [Presentation] Prospective Colonal Isolation of Human Mesenchymal Stem Cells Elucidates Heterogeneity within the Multi-potent Stem Cell (2010)
  • Author(s): 馬渕洋
  • Organizer: 8th Annuary Meeting ISSCR
  • Place of Presentation: アメリカMoscone West San Francisco, CA
  • Year and Date: 20100616-17
• [Presentation] Clonal isolation of human mesenchymal stem cell elucidates heterogeneity within the self-renewal and multi-potent stem cell compartment (2010)
  • Author(s): 松崎有未
  • Organizer: 第33回分子生物学会・日本生化学会
  • Place of Presentation: 兵庫県神戸ポートアイランド
  • Year and Date: 2010-12-10
• [Presentation] 間葉系幹細胞移植により惹起される慢性GVHD発症の新しいメカニズム (2010)
  • Author(s): 松崎有未
  • Organizer: 第32回幹細胞治療研究フォーラム
  • Place of Presentation: 東京都東京大学医科学研究所
  • Year and Date: 2010-12-09
• [Presentation] フローサイトメトリーによる高純度間葉系幹細胞の分離 (2010)
  • Author(s): 松崎有未
  • Organizer: 第25回日本整形外科学会
  • Place of Presentation: 京都府国立京都国際会館
  • Year and Date: 2010-10-15
• [Presentation] フローサイトメトリーによる高純度間葉系幹細胞の分離 (2010)
  • Author(s): 松崎有未
  • Organizer: 遺伝子医療学セミナー
  • Place of Presentation: 鳥取県鳥取大学
  • Year and Date: 2010-10-05
• [Presentation] セルソーターの原理 (2010)
  • Author(s): 松崎有未
  • Organizer: 理工学部講義
  • Place of Presentation: 東京都慶應義塾大学矢上キャンパス
  • Year and Date: 2010-10-02
• [Presentation] 間葉系幹細胞とiPS細胞 (2010)
  • Author(s): 松崎有未
  • Organizer: 日本炎症・再生医学会
  • Place of Presentation: 東京都京王プラザホテル
  • Year and Date: 2010-08-05
• [Presentation] 体性幹細胞を用いたiPS細胞誘導の高効率化 (2010)
  • Author(s): 松崎有未
  • Organizer: iPS・ES・体性幹細胞Forum 2010
  • Place of Presentation: 東京都秋葉原UDX GALLERY
  • Year and Date: 2010-06-29
• [Presentation] フローサイトメトリーを用いたヒト間葉系幹細胞の分離とクロナール解析 (2010)
  • Author(s): 松崎有未
  • Organizer: 第9回日本再生医療学会総会
  • Place of Presentation: 広島県広島国際会議場
  • Year and Date: 2010-03-19
• [Presentation] ヒト子宮内膜幹様細胞の同定・分離とその幹細胞特性の機能解析 (2009)
  • Author(s): 松崎有未
  • Organizer: 第37回日本臨床免疫学会総会
  • Place of Presentation: 東京都東京ステーションコンファレンス
  • Year and Date: 2009-11-14
• [Presentation] Direct isolation system of human mesenchymal stem cells by FCM (2009)
  • Author(s): 松崎有未
  • Organizer: Bio Japan 2009
  • Place of Presentation: 神奈川県パシフィコ横浜
  • Year and Date: 2009-10-08
• [Presentation] フローサイトメトリーを用いたヒト間葉系幹細胞の直接分離法開発 (2009)
  • Author(s): 松崎有未
  • Organizer: 第10回日本運動器科学研究会
  • Place of Presentation: 東京都東京ステーションコンファレンスサピアタワー
  • Year and Date: 2009-09-19
• [Presentation] 成体由来細胞から高品質iPS(induced Puripotent Stem)細胞の樹立に向けて (2009)
  • Author(s): 松崎有未
  • Organizer: 再生医療の実現化プロジェクト第2回夏のWS
  • Place of Presentation: 静岡県KKR熱海
  • Year and Date: 2009-07-17
• [Presentation] Prospective identification of Murine and Human Mesenchymal Stem Cells (2009)
  • Author(s): 松崎有未
  • Organizer: 7th Annual Meeting ISSCR
  • Place of Presentation: スペインCentre Convencions International Barcelona
  • Year and Date: 2009-07-10
• [Presentation] Prospective identification of Murine and Human Mesenchymal Stem Cells (2009)
  • Author(s): 松崎有未
  • Organizer: The 7th Stem Cell Research Symposium
  • Place of Presentation: 東京都慶應義塾大学医学部北里記念館
  • Year and Date: 2009-05-05
• [Book] SP(Side population)細胞炎症・再生医学事典 (2009)
  • Author(s): 松崎有未
  • Total Pages: 406-409
  • Publisher: 朝倉書店
• [Remarks]
  • URL: http://www.okano-lab.com/