Integrative analyses of circadian rhythms based on environment

2011 Fiscal Year Final Research Report

• Project/Area Number: 21390066
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Environmental physiology (including Physical medicine and Nutritional physiology)
• Research Institution: Hiroshima University
• Principal Investigator: TAKUMI Toru 広島大学, 大学院・医歯薬学総合研究科, 教授 (00222092)
• Project Period (FY): 2009 – 2011
• Keywords: 遺伝子 / 細胞・組織 / 環境 / 行動学 / 神経科学

Research Abstract

We use multiple high-throughput approaches including chromatin immunoprecipitation(ChIP)-based systematic analyses and DNA microarrays combined with bioinformatics, to generate genome-wide profiles of BMAL1 target genes. We reveal that, in addition to E-boxes, BMAL1 recognizes a consensus sequence CCAATG to elicit robust circadian expression. BMAL1 occupancy is found in more than 150 sites, including all known clock genes. Importantly, a significant proportion of BMAL1 targets include genes that encode central regulators of metabolic processes. The database generated in this study constitutes a useful resource to decipher the network of circadian gene control and its intimate links with several fundamental physiological functions

Research Products

• [Journal Article] REV-ERBa and the clock gene machinery in mouse peripheral tissues : a possible role as a synchronizing hing (2012)
  • Author(s): G. Mazzoccoli, Y. Cai, S. Liu, M. Francavilla, F. Giuliani, A. Piepoli, V. Pazienza, M. Vinciguerra, T. Yamamotoand T. Takumi
  • Journal Title: J. Biological Regulators & Homeostatic Agents, in press
  • Peer Reviewed
• [Journal Article] The period of the somite segmentation clock is sensitive to Notch activity (2011)
  • Author(s): W. Kim, T. Matsui, M. Yamao, M. Ishibashi, K. Tamada, T. Takumi, K. Kohno, S. Oba, S. Ishii, Y. Sakumura and Y. Bessho
  • Journal Title: Mol. Biol. Cell Volume: 22 Pages: 3541-3549
  • Peer Reviewed
• [Journal Article] Genome-wide profiling of the core clock protein BMAL1 targets reveals strict relationship with metabolism (2010)
  • Author(s): F. Hatanaka, C. Matsubara, J. Myung, T. Yoritaka, N. Kamimura, S. Tsutsumi, A. Kanai, Y. Suzuki, P. Sassone-Corsi, H. Aburatani, S. Sugano and T. Takumi
  • Journal Title: Mol. Cell. Biol Volume: 30 Pages: 5636-5648
  • Peer Reviewed
• [Journal Article] Decreased exploratory activity in the model mouse for 15q duplication syndrome (2010)
  • Author(s): K. Tamada, S. Tomonaga, F. Hatanaka, N. Nakai, K. Takao, T. Miyakawa, J. Nakatani and T. Takumi
  • Journal Title: implication in disturbance of serotonin signaling. PLoSONE Volume: 5 Pages: e15126
  • Peer Reviewed
• [Journal Article] 概日時計と気分障害 (2010)
  • Author(s): 内匠透
  • Journal Title: Clinical Neuroscience Volume: 28 Pages: 720-721
• [Journal Article] Abnormal behavior in a chromosome-engineering mouse model for human 15q11-13 duplication seen in autism (2009)
  • Author(s): J. Nakatani, K. Tamada, F. Hatanaka, S. Ise, H. Ohta, K. Inoue, S. Tomonaga, Y. Watanabe, Y. J. Chung, R. Banerjee, K. Iwamoto, T. Kato, M. Okazawa, K. Yamauchi, K. Tanda, K. Takao, T. Miyakawa, A. Bradley and T. Takumi
  • Journal Title: Cell Volume: 137 Pages: 1235-1246
  • Peer Reviewed
• [Journal Article] Fezf1 is required for penetration of the basal lamina by olfactory axons to promote olfactory development (2009)
  • Author(s): Y. Watanabe, K. Inoue, A. Okuyama-Yamamoto, N. Nakai, J. Nakatani, K.-i. Nibu, N. Sato, Y. Iiboshi, K. Yusa, G. Kondoh, J. Takeda, T. Terashimaand T. Takumi
  • Journal Title: J. Comp. Neurol. Volume: 515 Pages: 565-584
  • Peer Reviewed
• [Journal Article] Time microscopy of circadian expression of circadian clock gene mRNA transcription (2009)
  • Author(s): R. B. Sothern, G. Cornelissen, T. Yamamoto, T. Takumi and F. Halberg
  • Journal Title: chronodiagnostic and chrono-the rapeutic implications. Clin. Ter Volume: 160 Pages: ep25-34
  • Peer Reviewed
• [Journal Article] Central and peripheral circadian clock genes, their statistical analysis for rhythms, and relationship to health and disease (2009)
  • Author(s): R. B. Sothern, T. Yamamoto, G. Cornelissen, T. Takumi and F. Halberg
  • Journal Title: Scripta Med Volume: 82 Pages: 133-163
  • Peer Reviewed
• [Journal Article] Robust food anticipatory activity in BMAL1-deficient mice (2009)
  • Author(s): J. S. Pendergast, W. Nakamura, R. C. Friday, T. Takumi and S. Yamazaki
  • Journal Title: PLoS ONE Volume: 4 Pages: e4860
  • Peer Reviewed
• [Presentation] The phosphorylation of PERIOD2 via glycogen synthase kinase 3υhas the possibility of the regulator in the depression-like behavior (2011)
  • Author(s): Yousuke Yamawaki, Atsuko Takano, Toru Takumi
  • Organizer: The 32nd Naito Conference
  • Place of Presentation: Hokuto, Yamanashi
  • Year and Date: 20111018-21
• [Presentation] Rhythm and the blues Worldsleep 2011 (2011)
  • Author(s): Toru Takumi
  • Place of Presentation: Kyoto
  • Year and Date: 20111016-20
• [Presentation] Genome-wide profiling of the core clock protein BMAL1 targets reveals strict relationship with metabolism (2011)
  • Author(s): Fumiyuki Hatanaka, Chiaki Matsubara, Jihwan Myung, Takashi Yoritaka, NaokoUemura, Shuichi Tsutsumi, Akinori Kanai, Yutaka Suzuki, Hiroyuki Aburatani, SumioSugano, Toru Takumi
  • Organizer: 第34回日本神経科学大会
  • Place of Presentation: 横浜
  • Year and Date: 20110914-17
• [Presentation] 転写因子BMAL1のゲノム網羅的研究 (2011)
  • Author(s): 畠中史幸、松原千明、Jihwan Myung、寄高崇志、上村直子、堤修一、金井昭教、鈴木譲、油谷浩幸、菅野純夫、内匠透
  • Organizer: 第52回日本生化学会大会中国・四国支部例会
  • Place of Presentation: 広島
  • Year and Date: 20110513-14
• [Presentation] 転写因子BMAL1のゲノム網羅的研究 (2010)
  • Author(s): 畠中史幸、松原千明、寄高崇志、上村直子、堤修一、金井昭教、鈴木譲、油谷浩幸、菅野純夫、内匠透
  • Organizer: 第33回日本分子生物学会年会、第83回日本生化学会大会合同大会
  • Place of Presentation: 神戸
  • Year and Date: 20101207-10
• [Presentation] Alterations of locomotor activity dynamics in circadian-clock mutant mice. EMBL Workshop on Translating Behaviour (2009)
  • Author(s): T. Nakamura, T. Takumi, A. Takano, H. Kikuchi, and Y. Yamamoto
  • Organizer: Bridging Clinical and Animal Model Research. EMBL, Heidelberg
  • Place of Presentation: Germany
  • Year and Date: 20091114-16
• [Presentation] 時計と気分 (2009)
  • Author(s): 内匠透
  • Organizer: 第62回日本自律神経学会総会
  • Place of Presentation: 和歌山
  • Year and Date: 20091105-06
• [Presentation] Molecular correlations between circadian rhythm and mood disorder (2009)
  • Author(s): Toru Takumi
  • Organizer: 第82回日本生化学会大会
  • Place of Presentation: 神戸
  • Year and Date: 20091021-24
• [Presentation] うつ病ラットモデルを用いたリチウムシグナルとサーカディアンリズム解析 (2009)
  • Author(s): 高野敦子、内匠透
  • Organizer: 第82回日本生化学会大会
  • Place of Presentation: 神戸
  • Year and Date: 20091021-24
• [Presentation] Molecular interaction between circadian rhythm and mood disorders (2009)
  • Author(s): Toru Takumi
  • Organizer: XI Congress of the European Biological Rhythms Society
  • Place of Presentation: Strasbourg, France
  • Year and Date: 20090822-28
• [Presentation] Circadian biology of SCN (2009)
  • Author(s): Toru Takumi
  • Organizer: XXXVI International Congress of Physiological Sciences
  • Place of Presentation: Kyoto
  • Year and Date: 20090727-0801
• [Book] リズムと精神疾患.時間生物学(海老原史樹史、吉村崇編) (2012)
  • Author(s): 内匠透
  • Total Pages: 103-104
  • Publisher: 化学同人、京都
• [Book] 体内時計のからくり(NPO法人脳の世紀推進会議編.) (2010)
  • Author(s): 内匠透
  • Total Pages: 75-96
  • Publisher: クバプロ.東京
• [Remarks]
  • URL: http://home.hiroshima-u.ac.jp/anatomy2/index.html