Role of hypothalamic AMP kinase in food intake and metabolic regulation in lean and obese animals

2011 Fiscal Year Final Research Report

• Project/Area Number: 21390067
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Environmental physiology (including Physical medicine and Nutritional physiology)
• Research Institution: National Institute for Physiological Sciences
• Principal Investigator: MINOKOSHI Yasuhiko 生理学研究所, 発達生理学研究系, 教授 (10200099)
• Co-Investigator(Renkei-kenkyūsha): OKAMOTO Shiki 生理学研究所, 発達生理学研究系, 助教 (40342919) ; SHIUCHI Tetsuya 徳島大学, 大学院・ヘルスバイオサイエンス研究部, 講師 (70372729)
• Project Period (FY): 2009 – 2011
• Keywords: 生理学 / 栄養学 / 生体分子 / 摂食行動 / 肥満

Research Abstract

AMP kinase(AMPK) has been known to be a "metabolic sensor". Here, we found that AMPK in the paraventricular hypothalamus regulates food selection behavior for carbohydrate and fat diets via fatty acid oxidation in the nucleus.

Research Products

• [Journal Article] Lack of TRPM2 impaired insulin secretion and glucose metabolisms in mice (2011)
  • Author(s): Uchida K, Dezaki K, Damdindorj B, Inada H, Shiuchi T, Mori Y, Yada T, Minokoshi Y, Tominaga M
  • Journal Title: Diabetes Volume: 60 Pages: 119-126
  • DOI: doi:10.2337/db10-0276
  • Peer Reviewed
• [Journal Article] Decreased intake of sucrose solutions in orexin knockout mice (2011)
  • Author(s): Matsuo E, Mochizuki A, Nakayama K, Nakamura S, Yamamoto T, Shioda S, Sakurai T, Yanagisawa M, Shiuchi T, Minokoshi Y, Inoue T
  • Journal Title: J Mol Neurosci Volume: 43 Pages: 217-224
  • DOI: doi:10.1007/s12031-010-9475-1
  • Peer Reviewed
• [Journal Article] An enzymatic photometric assay for 2-deoxyglucose uptake in insulin-responsive tissues and 3T3-L1 adipocytes (2011)
  • Author(s): Saito K, Lee S, Shiuchi T, Toda C, Kamijo M, Inagaki-Ohara K, Okamoto S, Minokoshi Y
  • Journal Title: Analytical Biochem Volume: 412 Pages: 9-17
  • DOI: doi:10.1016/j.ab.2011.01.022
  • Peer Reviewed
• [Journal Article] DNA methylation of intronic enhancers directs tissue-specific expression of steroidogenic factor 1/adrenal 4 binding protein(SF-1/Ad4BP) (2011)
  • Author(s): Hoivik EA, Bjanesoy TE, Mai O, Shima Y, Okamoto S, Minokoshi Y, Morohashi K, Boehm U, Bakke M
  • Journal Title: Endocrinology Volume: 152 Pages: 2100-2112
  • DOI: doi:10.1210/en.2010-1305
  • Peer Reviewed
• [Journal Article] PDK1-Foxo1 in Agouti-related peptide neurons regulates energy homeostasis by modulating food intake and energy expenditure (2011)
  • Author(s): Cao Y, Nakata M, Okamoto S, Takano E, Yada T, Minokoshi Y, Hirata Y, Nakajima K, Iskandar K, Hayashi Y, Ogawa W, Barsh GS, Hosoda H, Kangawa K, Itoh H, Noda T, Kasuga M, Nakae J
  • Journal Title: PLoS ONE Volume: 6 Pages: e18324
  • DOI: doi:10.1371/journal.pone.0018324
  • Peer Reviewed
• [Journal Article] Role of central leptin signaling in the starvation-induced alteration of B cell development (2011)
  • Author(s): Tanaka M, Suganami T, Kim-Saijo M, Toda C, Tsuiji M, Ochi K, Kamei Y, Minokoshi Y, Ogawa Y
  • Journal Title: J Neurosci Volume: 31 Pages: 8373-8380
  • DOI: doi:10.1523/JNEUROSCI.6562-10.2011
  • Peer Reviewed
• [Journal Article] Structural basis for compound C inhibition of the human AMP-activated protein kinaseα2 subunit kinase domain (2011)
  • Author(s): Handa N, Takagi T, Saijo S, Kishishita S, Takaya D, Toyama M, Terada T, Shirouzu M, Suzuki A, Lee S, Yamauchi T, Okada-Iwabu M, Iwabu M, Kadowaki T, Minokoshi Y, Yokoyam S
  • Journal Title: Acta Crystallogr D Biol Crystallogr Volume: 67 Pages: 480-487
  • DOI: doi:10.1107/S0907444911010201
  • Peer Reviewed
• [Journal Article] Crystal structure of the Ca^<2+>/calmodulin-dependent protein kinase kinase in complex with the inhibitor STO-609 (2011)
  • Author(s): Kukimoto-Niino M, Yoshikawa S, Takagi T, Tomabechi Y, Terada T, Shirouzu M, Suzuki A, Lee S, Kadowaki T, Minokoshi Y, Yokoyama S
  • Journal Title: J Biol Chem Volume: 286 Pages: 22570-22579
  • DOI: doi:10.1074/jbc.M111.251710
  • Peer Reviewed
• [Journal Article] Metabolic adaptation of mice in a cool environment (2010)
  • Author(s): Uchida K, Shiuchi T, Inada H, Minokoshi Y, Tominaga, M
  • Journal Title: Pflugers Arch Volume: 459 Pages: 765-774
  • DOI: doi:10.1007/s00424-010-0795-3
  • Peer Reviewed
• [Journal Article] Induction of hypothalamic Sirt1 leads to cessation of feeding via agouti-related peptide (2010)
  • Author(s): Sasaki T, Kim HJ, Kobayashi M, Kitamura YI, Yokota-Hashimoto H, Shiuchi T, Minokoshi Y, Kitamura T
  • Journal Title: Endocrinology Volume: 151 Pages: 2556-2566
  • DOI: doi:10.1210/en.2009-1319
  • Peer Reviewed
• [Journal Article] CXCL14 deficiency in mice attenuates obesity and inhibits feeding behavior in a novel environment (2010)
  • Author(s): Tanegashima K, Okamoto S, Nakayama Y, Taya C, Shitara H, Ishii R, Yonekawa H, Minokoshi Y, Hara T
  • Journal Title: PLoS One Volume: 5 Pages: e10321
  • DOI: doi:10.1371/journal.pone.0010321
  • Peer Reviewed
• [Journal Article] A liver-derived secretory protein, Selenoprotein P, causes insulin resistance (2010)
  • Author(s): Misu H, Takamura T, Takayama H, Hayashi H, Matsuzawa-Nagata N, Kurita S, Ishikura K, Ando H, Takeshita Y, Ota T, Sakurai M, Yamashita T, Mizukoshi E, Yamashita T, Honda M, Miyamoto K, Kubota T, Kubota N, Kadowaki T, Kim H-J, Lee I-K, Minokoshi Y, Saito Y
  • Journal Title: Cell Metabolism Volume: 12 Pages: 483-495
  • DOI: doi:10.1016/j.cmet.2010.09.015
  • Peer Reviewed
• [Journal Article] Neuronal protein tyrosine phosphatase 1B deficiency results in inhibition of hypothalamic AMPK and isoform-specific activation of AMPK in peripheral tissues (2009)
  • Author(s): Xue B, Pulinilkunnil T, Murano I, Bence KK, He H, Minokoshi Y, Asakura K, Lee A, Haj F, Furukawa N, Catalano KJ, Delibegovic M, Balschi JA, Cinti S, Neel BG, Kahn BB
  • Journal Title: Mol Cell Biol Volume: 29 Pages: 4563-4573
  • DOI: doi:10.1128/MCB.01914-08
  • Peer Reviewed
• [Journal Article] Distinct effects of leptin and a melanocortin receptor agonist injected into medial hypothalamic nuclei on glucose uptake in peripheral tissues (2009)
  • Author(s): Toda C, Shiuchi T, Lee S, Yamato-Esaki M, Fujino Y, Suzuki A, Okamoto S, Minokoshi Y
  • Journal Title: Diabetes Volume: 58 Pages: 2757-2765
  • DOI: doi:10.2337/db09-0638
  • Peer Reviewed
• [Journal Article] Hypothalamic orexin stimulates feeding-associated glucose utilization in skeletal muscle via sympathetic nervous system (2009)
  • Author(s): Shiuchi T, Haque MS, Okamoto S, Inoue T, Kageyama H, Lee S, Toda C, Suzuki A, Bachman ES, Kim Y-B, Sakurai T, Yanagisawa M, Shioda S, Imoto K, Minokoshi Y
  • Journal Title: Cell Metabolism Volume: 10 Pages: 466-480
  • DOI: doi:10.1016/j.cmet.2009.09.013
  • Peer Reviewed
• [Presentation] AMP-kinase in the paraventricular hypothalamus regulates food selection behavior in mice (2012)
  • Author(s): Okamoto S, Minokoshi Y
  • Organizer: The 89th Annual Meeting of the Physiological Society of Japan
  • Place of Presentation: Matsumoto Bunka Kaikan, Matsumoto
  • Year and Date: 2012-03-31
• [Presentation] Hypothalamic and sympathetic control of muscle metabolism (2011)
  • Author(s): Minokoshi Y, Shiuchi T
  • Organizer: FASEB Summer Research Conferences "Glucose transporters, signaling, and Diabetes"
  • Place of Presentation: Snowmass Village Conference Center, Snowmass Village, Colorado, USA
  • Year and Date: 2011-08-14
• [Presentation] 視床下部AMPKによるエネルギー代謝調節機構 (2011)
  • Author(s): 箕越靖彦
  • Organizer: 第30回成長ホルモン成長因子セミナー
  • Place of Presentation: 新宿住友ホール、新宿区、東京都
  • Year and Date: 2011-06-10
• [Presentation] 視床下部AMPキナーゼによる摂食調節機構 (2011)
  • Author(s): 箕越靖彦
  • Organizer: 日本分子生物学会第10回春季シンポジウム
  • Place of Presentation: 石川県立音楽堂交流ホール、金沢市、石川県
  • Year and Date: 2011-05-25
• [Presentation] 食から肥満とやせのなぞを探る～摂食と代謝調節の脳内機構～、市民公開講座「考えよう!食と健康」 (2010)
  • Author(s): 箕越靖彦
  • Organizer: 日本栄養改善学会中国支部主催
  • Place of Presentation: 県立広島大学、広島市、広島県
  • Year and Date: 2010-12-11
• [Presentation] Regulatory role of hypothalamic AMP-kinase in calorie intake and food preference (2010)
  • Author(s): Minokoshi Y, Okamoto S
  • Organizer: The FASEB Summer Research Conference
  • Place of Presentation: Otsu Prince Hotel, Otsu, Shiga
  • Year and Date: 2010-10-07
• [Presentation] 肥満マウスの食餌嗜好性に及ぼす視床下部室傍核AMPキナーゼの調節作用 (2010)
  • Author(s): 岡本士毅、箕越靖彦
  • Organizer: 第31回日本肥満学会
  • Place of Presentation: 前橋テルサ、前橋市、群馬県
  • Year and Date: 2010-10-01
• [Presentation] Regulatory role of AMP-kinase in the paraventricular hypothalamus in calorie intake and food preference (2010)
  • Author(s): Minokoshi Y, Okamoto S
  • Organizer: 11th International Congress on Obesity. Satellite Symposium
  • Place of Presentation: Clarion Hotel, Stockholm, Sweden
  • Year and Date: 2010-07-10
• [Presentation] Role of he paraventricular hypothalamic AMP kinase in food selection behavior (2010)
  • Author(s): Minokoshi Y, Okamoto S
  • Organizer: The 87th Annual Meeting of the Physiological Society of Japan
  • Place of Presentation: Morioka Shimin-Bunka Hole,盛岡市,岩手県
  • Year and Date: 2010-05-21
• [Presentation] 摂食・代謝調節の脳内機構と栄養環境との相互作用 (2010)
  • Author(s): 箕越靖彦
  • Organizer: 脳科学研究教育センターシンポジウム
  • Place of Presentation: 北海道大学、札幌市、北海道
  • Year and Date: 2010-04-07
• [Presentation] Role of hypothalamic AMP kinase in food selection behavior (2010)
  • Author(s): Minokoshi Y, Okamoto S
  • Organizer: ICE2010 14th International Congress of Endocrinology, Symposium AMP kinase
  • Place of Presentation: Kyoto International Conference Center, Kyoto
  • Year and Date: 2010-03-30
• [Presentation] 視床下部室傍核AMPキナーゼによる食餌嗜好性調節機構 (2009)
  • Author(s): 箕越靖彦
  • Organizer: 第63回日本栄養・食糧学会大会、シンポジウム「中枢と末梢を結ぶ摂食調節機構」
  • Place of Presentation: 長崎市茂里町ブリックホール、長崎市、長崎県
  • Year and Date: 2009-05-20
• [Presentation] Role of a novel protein phosphatase CIPP in leptin-induced suppression of AMPK activity in the arcuate hypothalamus (2009)
  • Author(s): Minokoshi Y
  • Organizer: 2009 Nanjing Symposium on Obesity & Diabetes
  • Place of Presentation: Nanjing University, Nanjing, China
  • Year and Date: 2009-05-16
• [Presentation] Regulatory role of AMP-kinase in the paraventricular hypothalamus in calorie intake and food preference (2009)
  • Author(s): Minokoshi Y
  • Organizer: NRL International Mini-Symposium "A Recent Progress in the Research of Obesity and Related Metabolic Disorder"
  • Place of Presentation: Asan Medial Center, Seoul, Korea
  • Year and Date: 2009-05-14
• [Presentation] 視床下部AMPキナーゼによる食餌嗜好性の調節作用 (2009)
  • Author(s): 岡本士毅、箕越靖彦
  • Organizer: 第82回日本内分泌学会
  • Place of Presentation: 群馬県民会館、前橋市、群馬県
  • Year and Date: 2009-04-24
• [Remarks]
  • URL: http://www.nips.ac.jp/endocrlin/