Analysis of infection mechanisms of hepatitis C virus and establishment of the indicator cell lines for viral infection

2011 Fiscal Year Final Research Report

• Project/Area Number: 21390138
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Virology
• Research Institution: Osaka University
• Principal Investigator: MATSUURA Yoshiharu 大阪大学, 微生物病研究所, 教授 (50157252)
• Project Period (FY): 2009 – 2011
• Keywords: HCV / 複製 / 宿主因子 / マイクロRNA

Research Abstract

A liver-specific microRNA, miR-122, has been shown to participate in the efficient replication of hepatitis C virus(HCV). In this study, we examined the possibility of establishing a new permissive cell line for HCV propagation by the expression of miR-122. Among the cell lines we examined, Hep3B cells stably expressing miR-122(Hep3B/ miR-122) exhibited a significant enhancement of HCVcc propagation. Furthermore, a line of" cured" cells established by elimination of HCV RNA from the Hep3B/ miR-122 replicon cells exhibited an enhanced expression of miR-122.

Research Products

• [Journal Article] Dysfunction of autophagy participates in vacuole formation and cell death in cells replicating hepatitis C virus (2011)
  • Author(s): Taguwa S., Kambara H., Fujita N., Noda T., Yoshimori T., Koike K., Moriishi K., and Matsuura Y.
  • Journal Title: J. Virol Volume: 85 Pages: 13185-13194
  • DOI: doi:10.1128/JVI.06099-11
  • Peer Reviewed
• [Journal Article] Heterogeneous nuclear ribonucleoprotein A2 participates in the replication of Japanese encephalitis virus through an interaction with viral proteins and RNA (2011)
  • Author(s): Katoh H., Mori Y., Kambara H., Abe T., Fukuhara T., Morita E., Moriishi K., and Kamitani W., and Matsuura Y.
  • Journal Title: J. Virol Volume: 85 Pages: 10976-10988
  • DOI: doi:10.1128/JVI.00846-11
  • Peer Reviewed
• [Journal Article] Structure of hepatitis E viral particle (2011)
  • Author(s): Mori Y., and Matsuura Y.
  • Journal Title: Virus Res Volume: 61 Pages: 59-64
  • DOI: doi:10.1016/j.virusres.2011.03.015
  • Peer Reviewed
• [Journal Article] Involvement of cyclophilin B in the replication of Japanese encephalitis virus (2011)
  • Author(s): Kambara H., Tani H., Mori Y., Abe T., Katoh H., Fukuhara T., Taguwa S., Moriishi K, and Matsuura Y.
  • Journal Title: Virology Volume: 412 Pages: 211-219
  • DOI: doi:10.1016/j.virol.2011.01.011
  • Peer Reviewed
• [Journal Article] Elimination of hepatitis C virus from hepatocytes by a selective activation of therapeutic molecules (2011)
  • Author(s): Wen X., Abe T., Kukihara H., Taguwa S., Mori Y., Tani H., Kato N., Suzuki T., Tatsumi M., Moriishi M., and Matsuura Y.
  • Journal Title: PLoS One Volume: 6 Pages: e15967
  • DOI: doi:10.1371/journal.pone.0015967
  • Peer Reviewed
• [Journal Article] Intracellular delivery of serum-derived hepatitis C virus (2011)
  • Author(s): Fukuhara T., Tani H., Shiokawa M., Goto Y., Abe T., Taketomi A., Shirabe K., Maehara Y., Matsuura Y.
  • Journal Title: Microbes Infect Volume: 13 Pages: 405-412
  • DOI: doi:10.1016/j.micinf.2011.01.005
  • Peer Reviewed
• [Journal Article] Involvement of PA28g in the propagation of hepatitis C virus (2010)
  • Author(s): Moriishi K., Shoji I., Mori Y., Suzuki R., Suzuki T., Kataoka C., and Matsuura Y.
  • Journal Title: Hepatology Volume: 52 Pages: 411-420
  • DOI: doi:10.1002/hep.23680
  • Peer Reviewed
• [Journal Article] Involvement of ceramide in the propagation of Japanese encephalitis virus (2010)
  • Author(s): Tani H., Shiokawa M., Kaname Y., Kambara H., Mori Y., Abe T., Moriishi K., and Matsuura Y.
  • Journal Title: J. Virol Volume: 84 Pages: 2798-2807
  • DOI: doi:10.1128/JVI.02499-09
  • Peer Reviewed
• [Journal Article] Animal models for hepatitis C and related liver disease (2010)
  • Author(s): Koike K., Moriya K., and Matsuura Y.
  • Journal Title: Hepatology Res. Volume: 40 Pages: 69-82
  • DOI: doi:10.1111/j.1872-034X.2009.00593.x
  • Peer Reviewed
• [Journal Article] Co-chaperone activity of human butyrate-induced transcript 1 facilitates hepatitis C virus replication through an Hsp90-dependent pathway (2009)
  • Author(s): Taguwa S., Kambara H., Omori H., Tani H., Abe T., Mori Y., Suzuki T., Yoshimori T., Moriishi K., and Matsuura Y.
  • Journal Title: J. Virol Volume: 83 Pages: 10427-10436
  • DOI: doi:10.1128/JVI.01035-09
  • Peer Reviewed
• [Journal Article] Biological and immunological characteristics of hepatitis E virus-like particles based on the crystal structure (2009)
  • Author(s): Yamashita T., Mori Y., Miyazaki N., Cheng H. R., Yoshimura M., Unno H., Shima R., Moriishi K., Tsukihara T., Li T. C., Takeda N., Miyamura T., and Matsuura Y.
  • Journal Title: Proc. Natl. Acad. Sci. USA Volume: 106 Pages: 12986-12991
  • DOI: doi:10.1073/pnas.0903699106
  • Peer Reviewed
• [Journal Article] Human VAP-C negatively regulates hepatitis C virus propagation (2009)
  • Author(s): Kukihara H., Moriishi K., Taguwa S., Tani H., Abe T., Mori Y., Suzuki T., Fukuhara T., Taketomi A., Maehara Y., and Matsuura Y.
  • Journal Title: J. Virol Volume: 83 Pages: 7959-7969
  • DOI: doi:10.1128/JVI.00889-09
  • Peer Reviewed
• [Journal Article] Establishment of a novel permissive cell line for propagation of hepatitis C virus by the expression of microRNA122
  • Author(s): Kambara H., Fukuhara T., Shiokawa M., Ono C., Ohara Y., Kamitani W., and Matsuura Y.
  • Journal Title: J. Virol Volume: 86 Pages: 1382-1393
  • DOI: doi:10.1128/JVI.00567-12
  • Peer Reviewed
• [Presentation] Heterogeneous nuclear ribonucleoprotein A2 participates in the replication of Japanese encephalitis virus through the interaction with viral proteins and RNA (2011)
  • Author(s): Katoh H, Mori Y, Kambara H, Kamitani W, and Matsuura Y.
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: 横浜
  • Year and Date: 20111213-16
• [Presentation] miR122 participates in the determination of cell tropism of hepatitis C virus (2011)
  • Author(s): Fukuhara T, Kambara H, Shiokawa M, Ohara Y, Ono C, and Matsuura Y.
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: 横浜
  • Year and Date: 20111213-16
• [Presentation] miR122 facilitates replication of hepatitis C virus in non-hepatic cells (2011)
  • Author(s): Fukuhara T, Shiokawa M, Ninomiya A, Kambara H, Katoh H, Morita E, Wataru Kamitani W, and Matsuura Y.
  • Organizer: 第59回日本ウイルス学会総会
  • Place of Presentation: 札幌
  • Year and Date: 20110912-16
• [Presentation] Annexins negatively regulate HCV RNA replication (2011)
  • Author(s): Abe T, Fukuhara T, Morita E, and Matsuura Y.
  • Organizer: 18th International Meeting on HCV and Related Viruses
  • Place of Presentation: Seattle
  • Year and Date: 20110908-12
• [Presentation] miR122 facilitates replication of hepatitis C virus in non-hepatic cells (2011)
  • Author(s): Fukuhara T, Shiokawa M, Ono C, Kambara H, Morita E, Kamitani W, and Matsuura Y.
  • Place of Presentation: Seattle
  • Year and Date: 20110908-12
• [Presentation] C型肝炎ウイルスの増殖と病原性に関与する宿主因子 (2011)
  • Author(s): 松浦善治
  • Organizer: 第48回日本ウイルス学会九州支部総会
  • Place of Presentation: 門司
  • Year and Date: 20110826-27
• [Presentation] Heterogeneous nuclear ribonucleoprotein A2 participates in the replication of Japanese encephalitis virus through the interaction with viral proteins and RNA (2011)
  • Author(s): Katoh H, Mori Y, Kambara H, Kamitani W, and Matsuura Y.
  • Organizer: The American Society for Virology, 30th Annual Meeting, University of Minnesota
  • Place of Presentation: Minnesota
  • Year and Date: 20110716-20
• [Presentation] miR122 facilitates replication of hepatitis C virus in non-hepatic cells (2011)
  • Author(s): Fukuhara T, Shiokawa M, Ninomiya A, Kambara H, Katoh H, Morita E, Wataru Kamitani W, and Matsuura Y.
  • Year and Date: 20110716-20
• [Presentation] C型肝炎・肝癌制圧の分子基盤 (2011)
  • Author(s): 松浦善治
  • Organizer: 第47回日本肝臓学会総会
  • Place of Presentation: 東京
  • Year and Date: 20110602-03
• [Presentation] C型肝炎ウイルスの増殖と病原性発現に関与する宿主因子～細胞内蛋白質分解システムの関与について～ (2011)
  • Author(s): 松浦善治
  • Organizer: 第10回Hepatitis Expert Meeting
  • Place of Presentation: 東京
  • Year and Date: 2011-08-27
• [Presentation] C型肝炎ウイルス感染による肝細胞癌の発症に関与する宿主因子 (2010)
  • Author(s): 松浦善治
  • Organizer: 第33回日本分子生物学会年会
  • Place of Presentation: 神戸
  • Year and Date: 20101207-10
• [Presentation] C型肝炎ウイルスはオートファジーを誘導して持続感染を成立させる (2010)
  • Author(s): 寒原裕登、田鍬修平、藤田尚信、野田健司、森石恆司、吉森保、松浦善治
  • Organizer: 第58回日本ウイルス学会総会
  • Place of Presentation: 徳島
  • Year and Date: 20101107-09
• [Presentation] C型肝炎ウイルスの細胞侵入におけるフォスフォリパーゼCおよびプロテインキナーゼC依存的なシグナル伝達経路の関与 (2010)
  • Author(s): 谷英樹、阿部隆之、森石恆司、松浦善治
  • Organizer: 第58回日本ウイルス学会総会
  • Place of Presentation: 徳島
  • Year and Date: 20101107-09
• [Presentation] IL28B遺伝子多型と肝移植後のインターフェロン感受性 (2010)
  • Author(s): 福原崇介、本村貴志、二宮彰紀、阿部隆之、武冨紹信、前原喜彦、松浦善治
  • Organizer: 第58回日本ウイルス学会総会
  • Place of Presentation: 徳島
  • Year and Date: 20101107-09
• [Presentation] 不死化ヒト肝細胞株(Hc細胞)への患者血清由来HCVの感染 (2010)
  • Author(s): 塩川舞、福原崇介、後藤志典、二宮彰紀、谷英樹、阿部隆之、森石恆司、松浦善治
  • Organizer: 第58回日本ウイルス学会総会
  • Place of Presentation: 徳島
  • Year and Date: 20101107-09
• [Presentation] HCVによる脂質代謝障害の分子機序 (2010)
  • Author(s): 森石恆司、松浦善治
  • Organizer: 第58回日本ウイルス学会総会
  • Place of Presentation: 徳島
  • Year and Date: 20101107-09
• [Presentation] C型肝炎ウイルス感染細胞特異的なウイルス排除システムの構築 (2010)
  • Author(s): 温暁玉、阿部隆之、久木原博、田鍬修平、森嘉生、谷英樹、加藤宣之、鈴木哲朗、巽正志、森石恆司、松浦善治
  • Organizer: 第58回日本ウイルス学会総会
  • Place of Presentation: 徳島
  • Year and Date: 20101107-09
• [Presentation] C型肝炎ウイルスのtrans-packaging型粒子を用いた感染機構の解析 (2010)
  • Author(s): 鈴木亮介、斎藤憲司、赤澤大輔、石井孝司、松浦善治、脇田隆字、鈴木哲朗
  • Organizer: 第58回日本ウイルス学会総会
  • Place of Presentation: 徳島
  • Year and Date: 20101107-09
• [Presentation] 細胞内アネキシンはC型肝炎ウイルスの複製を制御する (2010)
  • Author(s): 阿部隆之、谷英樹、森石恆司、松浦善治
  • Organizer: 第58回日本ウイルス学会総会
  • Place of Presentation: 徳島
  • Year and Date: 20101107-09
• [Presentation] 核小体蛋白質B23はC型肝炎ウイルスの複製を抑制する (2010)
  • Author(s): 加藤大志、森嘉生、寒原裕登、要祐喜、谷英樹、阿部隆之、神谷亘、森石恆司、松浦善治
  • Organizer: 第58回日本ウイルス学会総会
  • Place of Presentation: 徳島
  • Year and Date: 20101107-09
• [Presentation] 温故知新・C型肝炎ウイルス研究の源流 (2010)
  • Author(s): 松浦善治
  • Organizer: 第52回日本消化器病学会大会
  • Place of Presentation: 横浜
  • Year and Date: 20101013-16
• [Presentation] Host factors involved in the propagation and pathogenesis of HCV (2010)
  • Author(s): Matsuura Yoshiharu
  • Organizer: 17thth International Meeting on HCV and Related Viruses
  • Place of Presentation: 横浜
  • Year and Date: 20100910-14
• [Presentation] Inhibition of autophagy induces lysosomal vacuolation in cells replicating HCV (2010)
  • Author(s): Hiroto Kambara, Shuhei Taguwa, Naonobu Fujita, Takeshi Noda, Tamotsu Yoshimori Kohji Moriishi, and Yoshiharu Matsuura
  • Organizer: 17thth International Meeting on HCV and Related Viruses
  • Place of Presentation: 横浜
  • Year and Date: 20100910-14
• [Presentation] HCV replication enhances secretion of an immature cathepsin B through induction of an incomplete autophagy (2010)
  • Author(s): Shuhei Taguwa, Hiroto Kambara, Naonobu Fujita, Takeshi Noda, Tamotsu Yoshimori Kohji Moriishi, and Yoshiharu Matsuura
  • Organizer: 17thth International Meeting on HCV and Related Viruses
  • Place of Presentation: 横浜
  • Year and Date: 20100910-14
• [Presentation] Involvement of PA28gamma in the propagation of HCV (2010)
  • Author(s): Kohji Moriishi, Ikuo Shoji, Yoshio Mori, Ryosuke Suzuki, Tetsuro Suzuki, Chikako Kataoka, and Yoshiharu Matsuura
  • Organizer: 17thth International Meeting on HCV and Related Viruses
  • Place of Presentation: 横浜
  • Year and Date: 20100910-14
• [Presentation] IL28B variation in recipients and donors correlates with response to peg-interferon/ ribavirn for recurrent hepatitis C (2010)
  • Author(s): Takasuke Fukuhara, Akinobu Taketomi, Takashi Motomura, Akinori Ninomiya, Takayuki Abe, Yoshihiko Maehara, Yoshiharu Matsuura
  • Organizer: 17thth International Meeting on HCV and Related Viruses
  • Place of Presentation: 横浜
  • Year and Date: 20100910-14
• [Presentation] A splice variant of CD44 participates in the IP-10 production in cells infected with HCV (2010)
  • Author(s): Takayuki Abe, Kohji Moriishi, and Yoshiharu Matsuura
  • Organizer: 17thth International Meeting on HCV and Related Viruses
  • Place of Presentation: 横浜
  • Year and Date: 20100910-14
• [Presentation] Involvement of phospholipase C and protein kinase C-dependent signaling pathways in the entry of HCV (2010)
  • Author(s): Hideki Tani, Takayuki Abe, Kohji Moriishi, and Yoshiharu Matsuura
  • Organizer: 17thth International Meeting on HCV and Related Viruses
  • Place of Presentation: 横浜
  • Year and Date: 20100910-14
• [Presentation] Autophagy is required for cell survival in cells replicating hepatitis C virus. The American Society for Virology (2010)
  • Author(s): Hiroto Kambara, Shuhei Taguwa, Naonobu Fujita, Kohji Moriishi, Takeshi Noda, Tamotsu Yoshimori, , and Yoshiharu Matsuura
  • Organizer: 29th Annual Meeting, Montana State University
  • Place of Presentation: Montana
  • Year and Date: 20100717-21
• [Presentation] Host factors involved in the replication of hepatitis C virus (2010)
  • Author(s): 松浦善治
  • Organizer: 第62回細胞生物学会大会
  • Place of Presentation: 大阪
  • Year and Date: 20100519-21
• [Presentation] C型肝炎ウイルスの感染におけるオートファジーの意義 (2009)
  • Author(s): 田鍬修平、寒原裕登、藤田尚信、野田健司、森石恆司、吉森保、松浦善治
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: 横浜
  • Year and Date: 20091209-12
• [Presentation] HCVコア蛋白質のプロテアソームによる分解とウイルス産生制御 (2009)
  • Author(s): 森石恆司、勝二郁夫、鈴木亮介、鈴木哲朗、松浦善治
  • Organizer: 第57回日本ウイルス学会総会
  • Place of Presentation: 東京
  • Year and Date: 20091025-27
• [Presentation] 日本脳炎ウイルスの感染における脂質セラミドの役割 (2009)
  • Author(s): 谷英樹、塩川舞、寒原裕登、要祐喜、森嘉生、森石恆司、松浦善治
  • Organizer: 第57回日本ウイルス学会総会
  • Place of Presentation: 東京
  • Year and Date: 20091025-27
• [Presentation] 患者血清由来HCVの細胞内導入法 (2009)
  • Author(s): 福原崇介、谷英樹、塩川舞、森石恆司、前原喜彦、松浦善治
  • Organizer: 第57回日本ウイルス学会総会
  • Place of Presentation: 東京
  • Year and Date: 20091025-27
• [Presentation] HCVの増殖とオートファジー (2009)
  • Author(s): 寒原裕登、田鍬修平、藤田尚信、森嘉生、野田健司、森石恆司、吉森保、松浦善治
  • Organizer: 第57回日本ウイルス学会総会
  • Place of Presentation: 東京
  • Year and Date: 20091025-27
• [Presentation] ヒアルロン酸による炎症性ケモカインIP-10の過剰産生とC型肝炎の慢性化 (2009)
  • Author(s): 阿部隆之、要祐喜、森石恆司、考藤達哉、林紀夫、松浦善治
  • Organizer: 第57回日本ウイルス学会総会
  • Place of Presentation: 東京
  • Year and Date: 20091025-27
• [Presentation] Hyaluronan participates in the IP-10 induction in cells infected with HCV through an engagement of TLR2 and CD44 (2009)
  • Author(s): Takayuki Abe, Yuuki Kaname, Kohji Moriishi, Tatsuya Kanto, Norio Hayashi, and Yoshiharu Matsuura
  • Organizer: 16th International Meeting on HCV and Related Viruses
  • Place of Presentation: Nice
  • Year and Date: 20091003-07
• [Presentation] Suppression of HCV replication in hepatocytes through a selective induction of IRF7 (2009)
  • Author(s): Xiaoyu Wen, Takayuki Abe, Shyuhei Taguwa, Yoshio Mori, Kohji Moriishi, and Yoshiharu Matsuura
  • Organizer: 16th International Meeting on HCV and Related Viruses
  • Place of Presentation: Nice
  • Year and Date: 20091003-07
• [Presentation] Involvement of PA28gamma-and E6AP-dependent degradation of HCV core protein in the viral production (2009)
  • Author(s): Kohji Moriishi, Ikuo Shoji, Ryosuke Suzuki, Tetsuro Suzuki, and Yoshiharu Matsuura
  • Organizer: 16th International Meeting on HCV and Related Viruses
  • Place of Presentation: Nice
  • Year and Date: 20091003-07
• [Presentation] Structure-based analysis of hepatitis E virus-like particle. The American Society for Virology (2009)
  • Author(s): Yoshio Mori, Tetsuo Yamashita, Naoyuki Miyazaki, Masato Yoshimura, Hideaki Unno, Kohji Moriishi, Tian-Cheng Li, Naokazu Takeda, R. Holland Cheng, Tomitake Tsukihara, and Yoshiharu Matsuura
  • Organizer: 28th Annual Meeting, The University of British Columbia
  • Place of Presentation: Vancouver
  • Year and Date: 20090711-15
• [Presentation] Human butyrate-induced transcript 1 interacts with both HCV NS5A and Hsp90 and regulates replication of hepatitis C virus (2009)
  • Author(s): Shuhei Taguwa, Kohji Moriishi, and Yoshiharu Matsuura
  • Organizer: 28th Annual Meeting, The University of British Columbia
  • Place of Presentation: Vancouver
  • Year and Date: 20090711-15
• [Presentation] Viral elimination by a selective expression of IRF7 in human hepatocytes infected with HCV (2009)
  • Author(s): Xiaoyu Wen, Takayuki Abe, Shyuhei Taguwa, Yoshio Mori, Kohji Moriishi, and Yoshiharu Matsuura
  • Organizer: 第15回日本遺伝子治療学会
  • Place of Presentation: 大阪
  • Year and Date: 20090610-12
• [Remarks] ホームページ
  • URL: http://www.biken.osaka-u.ac.jp/act/act_matsuura.php