2011 Fiscal Year Final Research Report
Mechanism of abnormalities in clinical laboratory data by analysis of epigenome, genome and miRome
| Project/Area Number |
21390180
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (B)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Laboratory medicine
|
|---|
| Research Institution | Hamamatsu University School of Medicine |
|---|
Principal Investigator |
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
TAKESHITA Akihiro 浜松医科大学, 医学部, 准教授 (00242769)
IINO Kazumi 浜松医科大学, 医学部附属病院, 助教 (90402263)
WATANABE Watanabe 浜松医科大学, 医学部, 助教 (00362187)
HASHIMOTO Dai 浜松医科大学, 医学部, 講師 (20467236)
FUJISAWA Tomouki 浜松医科大学, 医学部附属病院, 助教 (20402357)
|
|---|
| Project Period (FY) |
2009 – 2011
|
| Keywords | エピジェネティクス / マイクロRNA / ゲノム / 臨床検査値 |
|---|
| Research Abstract |
Even genuinely Le-negative patients, who genetically lack the Le enzyme and theoretically never produce CA19-9, occasionally show a slight increase in serum CA19-9 level. We analyzed this mechanism and found the phenomena are occurred when the patients are homozygous for Se-negative genotypes and suffer from advanced cancer with overproduction of glycans as precursors of CA19-9.
The human genome is composed of large-scale compartmentalized structures, including replication-timing zones and long-range GC% mosaic structures, which are related to chromosome bands. We found that the early/late-switch regions of replication timing, which generally correspond with transitions in GC content, are correlated with R/G-chromosome band boundaries, and such specific regions in the human genome correspond to "unstable" regions of the genome in which increased DNA damage occurs. Information acquired through genome-wide studies of replication timing will contribute substantially to our understanding of the molecular mechanisms in the pathogenesis of diseases.
|
-
-
-
-
-
[Journal Article] The Importance of Evaluation of DNA Amplificability in KRAS Mutation Testing with Dideoxy Sequencing using Formalin-fixed and Paraffin-embedded Colorectal Cancer Tissues2011
Author(s)
Okayama N, Nishioka M, Hazama S, Sakai K, Suehiro Y, Maekawa M, Sakamoto J, Iwamoto S, Kato T, Mishima H, Oka M, Hinoda Y
-
Journal Title
Japanese Journal of Clinical Oncology
Volume: 41(2)
Pages: 165-171
Peer Reviewed
-
[Journal Article] Plasma CCN2(connective tissue growth factor ; CTGF) is a potential biomarker in idiopathic pulmonary fibrosis(IPF)2011
Author(s)
Kono M, Nakamura Y, Suda T, Kato M, Kaida Y, Hashimoto D, Inui N, Hamada E, Miyazaki O, Kurashita S, Fukamachi I, Endo K, Ng P, Takehara K, Nakamura H Maekawa M, Chida K
-
Journal Title
Clinica Chimica Acta
Volume: 412
Pages: 2211-2215
Peer Reviewed
-
-
-
[Journal Article] Community of National Institute of Standard and Technology standard reference material 1955 homocysteine and folate in frozen human serum for total folate with automated assays2010
Author(s)
Ihara H, Watanabe T, Hashizume N, Totani M, Kamioka K, Onda K, Sunahara S, Suzuki T, Itabashi M, Aoki Y, Ishibashi M, Ito S, Ohashi K, Enomoto T, Saito K, Saeki K, Nagamura Y, Nobori T, Hirota K, Fujishiro K, Maekawa M, Miura M, Ohta Y
-
Journal Title
Annals of Clinical Biochemistry
Volume: 47
Pages: 541-548
Peer Reviewed
-
-
[Journal Article] Identification of 5 novel germline APC mutations and characterization of clinical phenotypes in Japanese patients with classical and attenuated familial adenomatous polyposis2010
Author(s)
Tao H, Shinmura K, Yamada H, Maekawa M, Osawa S, Takayanagi Y, Okamoto K, Terai T, Mori H, Nakamura T, Sugimura H
-
Journal Title
BMC Research Notes
Volume: 305(3)
Pages: 305
Peer Reviewed
-
[Journal Article] Possible Relevance between Prohormone Convertase 2 Expression and Tumor Growth in Human Adrenocorticotropin-Producing Pituitary Adenoma2010
Author(s)
Iino K, Oki Y, Yamashita M, Matsushita F, Hayashi C, Yogo K, Nishizawa S, Yamada S, Maekawa M, Sasano H, Nakamura H
-
Journal Title
Journal of Clinical Endocrinology and Metabolism
Volume: 95(8)
Pages: 4003-4011
Peer Reviewed
-
-
-
-
[Journal Article] CMC-544(inotuzumab ozogamicin) shows less effect on multidrug resistant cells : analyses in cell lines and cells from patients with B-cell chronic lymphocytic leukaemia and lymphoma2009
Author(s)
Takeshita A, Shinjo K, Yamakage N, Ono T, Hirano I, Matsui H, Shigeno K, Nakamura S, Tobita T, Maekawa M, Ohnishi K, Sugimoto Y, Kiyoi H, Naoe T, Ohno R
-
Journal Title
Br J Haematol
Volume: 146(1)
Pages: 34-43
Peer Reviewed
-
[Journal Article] CMC-544(inotuzumab ozogamicin), an anti-CD22 immuno-conjugate of calicheamicin, alters the levels of target molecules of malignant B-cells2009
Author(s)
Takeshita A, Yamakage N, Shinjo K, Ono T, Hirano I, Nakamura S, Shigeno K, Tobita T, Maekawa M, Kiyoi H, Naoe T, Ohnishi K, Sugimoto Y, Ohno R
-
Journal Title
Leukemia
Volume: 23(7)
Pages: 1372
Peer Reviewed
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
