A comprehensive transcriptome analysis of humanized mouse liver using ultra high density cDNA micro array

2011 Fiscal Year Final Research Report

• Project/Area Number: 21390229
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Gastroenterology
• Research Institution: Hiroshima University
• Principal Investigator: CHAYAMA Kazuaki 広島大学, 大学院・医歯薬学総合研究科, 教授 (00211376)
• Project Period (FY): 2009 – 2011
• Keywords: 肝臓学

Research Abstract

We investigated a mechanism of continuous infection of hepatitis B and hepatitis C virus with high density microarray using human hepatocyte chimeric mice. Induction of interferon stimulated genes were partially inhibited by each of the two viruses suggesting that these viruses inhibit induction of interferon stimulated genes with different mechanisms

Research Products

• [Journal Article] Severe necroinflammatory reaction caused by natural killer by Fas/ FasL interaction and dendritic cells in human hepatocyte chimeric mouse (2012)
  • Author(s): Okazaki A, Hiraga N, Imamura M, NelsonHayes C, Tsuge M, Takahashi S, AikataH, Abe H, Miki D, Ochi H, Tateno C, Yoshizato K, Ohdan H, Chayama K
  • Journal Title: Hepatology
  • DOI: doi:10.1002/hep.25651.
  • Peer Reviewed
• [Journal Article] Toward the establishment of a prediction system for thepersonalized treatment of chronic hepatitis C (2012)
  • Author(s): Ochi H, Hayes CN, Abe H, Hayashida Y, Uchiyama T, Kamatani N, Nakamura Y, Chayama K
  • Journal Title: J Infect Dis Volume: 205 Pages: 204-210
  • DOI: doi:10.1093/infdis/jir726.
  • Peer Reviewed
• [Journal Article] Telaprevir with peginterferon and ribavirin for treatment-naive patients chronically infected with HCV of genotype 1 inJapan (2012)
  • Author(s): Kumada H, Toyota J, Okanoue T, ChayamaK, Tsubouchi H, Hayashi N
  • Journal Title: J Hepatol Volume: 56 Pages: 78-84
  • DOI: doi:10.1016/j.jhep.2011.07.016.
  • Peer Reviewed
• [Journal Article] Dual therapy with the nonstructural protein 5A inhibitor, daclatasvir, and the nonstructural protein 3 protease inhibitor, asunaprevir, inhepatitis C virus genotype1b-infected null responders (2012)
  • Author(s): Chayama K, Takahashi S, Toyota J, Karino Y, Ikeda K, Ishikawa H, Watanabe H, McPhee F, Hughes E, KumadaH
  • Journal Title: Hepatology Volume: 55 Pages: 742-748
  • DOI: doi:10.1002/hep.24724.
  • Peer Reviewed
• [Journal Article] Will there be an HCV meeting in 2020? Summary of the 17th international meeting on hepatitis C virus and related viruses (2011)
  • Author(s): Wakita T, Suzuki T, Evans MJ, Shimotohno K, Chayama K, Matsuura Y, Hijikata M, Moriishi K, Seya T, Enomoto N, Koike K, Kato N, Kanto T, Hotta H
  • Journal Title: Gastroenterology Volume: 141 Pages: e1-5
  • DOI: doi:10.1053/j.gastro.2011.05.027.
  • Peer Reviewed
• [Journal Article] Effects of hepatitis B virus infection on the interferon response in immunodeficient human hepatocyte chimeric mice (2011)
  • Author(s): Tsuge M, Takahashi S, Hiraga N, Fujimoto Y, Zhang Y, Mitsui F, Abe H, Kawaoka T, Imamura M, Ochi H, Hayes CN, Chayama K
  • Journal Title: J Infect Dis Volume: 204 Pages: 224-228
  • DOI: doi:10.1093/infdis/jir247.
  • Peer Reviewed
• [Journal Article] Hepatitis C virus infectionsuppresses the interferon response inthe liver of the human hepatocytechimeric mouse (2011)
  • Author(s): Tsuge M, Fujimoto Y, Hiraga N, ZhangY, Ohnishi M, Kohno T, Abe H, Miki D, Imamura M, Takahashi S, Ochi H, HayesCN, Miya F, Tsunoda T, Chayama K
  • Journal Title: PLoS One Volume: 6 Pages: e23856
  • DOI: doi:10.1371/journal.pone.0023856.
  • Peer Reviewed
• [Journal Article] Influence of ITPA polymorphisms on decreases of hemoglobin during treatment with pegylated interferon, ribavirin, and telaprevir (2011)
  • Author(s): Suzuki F, Suzuki Y, Akuta N, Sezaki H, Hirakawa M, Kawamura Y, Hosaka T, Kobayashi M, Saito S, Arase Y, IkedaK, Kobayashi M, Chayama K, Kamatani N, Nakamura Y, Miyakawa Y, Kumada H
  • Journal Title: Hepatology Volume: 53 Pages: 415-421
  • DOI: doi:10.1002/hep.24058.
  • Peer Reviewed
• [Journal Article] In vivo adaptation of hepatitis C virus in chimpanzees for efficient virus production and evasion of apoptosis (2011)
  • Author(s): Saeed M, Shiina M, Date T, Akazawa D, Watanabe N, Murayama A, Suzuki T, Watanabe H, Hiraga N, Imamura M, Chayama K, Choi Y, Krawczynski K, Liang TJ, Wakita T, Kato T
  • Journal Title: Hepatology Volume: 54 Pages: 425-433
  • DOI: doi:10.1002/hep.24399.
  • Peer Reviewed
• [Journal Article] Impact of radiation and hepatitis virus infection on risk of hepatocellular carcinoma (2011)
  • Author(s): Ohishi W, Fujiwara S, Cologne JB, Suzuki G, Akahoshi M, Nishi N, TsugeM, Chayama K
  • Journal Title: Hepatology Volume: 53 Pages: 1237-1245
  • DOI: doi:10.1002/hep.24207.
  • Peer Reviewed
• [Journal Article] Elimination of hepatitis C virus by short term NS3-4A and NS5B inhibitor combination therapy in human hepatocyte chimeric mice (2011)
  • Author(s): Ohara E, Hiraga N, Imamura M, Iwao E, Kamiya N, Yamada I, Kono T, Onishi M, Hirata D, Mitsui F, Kawaoka T, TsugeM, Takahashi S, Abe H, Hayes CN, OchiH, Tateno C, Yoshizato K, Tanaka S, Chayama K
  • Journal Title: JHepatol Volume: 54 Pages: 872-878
  • DOI: doi:10.1016/j.jhep.2010.08.033.
  • Peer Reviewed
• [Journal Article] IL-28B predicts response to chronic hepatitis Ctherapy-fine-mapping and replication study in Asian populations (2011)
  • Author(s): Ochi H, Maekawa T, Abe H, Hayashida Y, Nakano R, Imamura M, Hiraga N, Kawakami Y, Aimitsu S, Kao JH, Kubo M, Tsunoda T, Kumada H, Nakamura Y, HayesCN, Chayama K
  • Journal Title: J Gen Virol Volume: 92 Pages: 1071-1081
  • DOI: doi:10.1099/vir.0.029124-0.
  • Peer Reviewed
• [Journal Article] Variation in the DEPDC5 locus is associated with progression to hepatocellular carcinoma in chronic hepatitis C virus carriers (2011)
  • Author(s): Miki D, Ochi H, Hayes CN, Abe H, Yoshima T, Aikata H, Ikeda K, KumadaH, Toyota J, Morizono T, Tsunoda T, Kubo M, Nakamura Y, Kamatani N, Chayama K
  • Journal Title: Nat Genet Volume: 43 Pages: 797-800
  • DOI: doi:10.1038/ng.876.
  • Peer Reviewed
• [Journal Article] A genome-wide association study of chronic hepatitis B identified novel risk locus in a Japanese population (2011)
  • Author(s): Mbarek H, Ochi H, Urabe Y, Kumar V, Kubo M, Hosono N, Takahashi A, Kamatani Y, Miki D, Abe H, Tsunoda T, Kamatani N, Chayama K, Nakamura Y, Matsuda K
  • Journal Title: Hum Mol Genet Volume: 20 Pages: 3884-3892
  • DOI: doi:10.1093/hmg/ddr301.
  • Peer Reviewed
• [Journal Article] Predictive value of the IL28B polymorphism on the effect of interferon therapy in chronic hepatitis C patients with genotypes 2a and 2b (2011)
  • Author(s): Kawaoka T, Hayes CN, Ohishi W, Ochi H, Maekawa T, Abe H, Tsuge M, Mitsui F, Hiraga N, Imamura M, Takahashi S, KuboM, Tsunoda T, Nakamura Y, Kumada H, Chayama K
  • Journal Title: J Hepatol Volume: 54 Pages: 408-414
  • DOI: doi:10.1016/j.jhep.2010.07.032.
  • Peer Reviewed
• [Journal Article] Rapidemergence of telaprevir resistanthepatitis C virus strain from wildtypeclone in vivo (2011)
  • Author(s): Hiraga N, Imamura M, Abe H, Hayes CN, Kono T, Onishi M, Tsuge M, TakahashiS, Ochi H, Iwao E, Kamiya N, Yamada I, Tateno C, Yoshizato K, Matsui H, KanaiA, Inaba T, Tanaka S, Chayama K
  • Journal Title: Hepatology Volume: 54 Pages: 781-788
  • DOI: doi:10.1002/hep.24460.
  • Peer Reviewed
• [Journal Article] Impact of viral aminoacid substitutions and hostinterleukin-28b polymorphism on replication and susceptibility tointerferon of hepatitis C virus (2011)
  • Author(s): Hiraga N, Abe H, Imamura M, Tsuge M, Takahashi S, Hayes CN, Ochi H, TatenoC, Yoshizato K, Nakamura Y, KamataniN, Chayama K
  • Journal Title: Hepatology Volume: 54 Pages: 764-711
  • DOI: doi:10.1002/hep.24453.
  • Peer Reviewed
• [Journal Article] HCV substitutions and IL28B polymorphisms on outcome ofpeg-interferon plus ribavirincombination therapy (2011)
  • Author(s): Hayes CN, Kobayashi M, Akuta N, SuzukiF, Kumada H, Abe H, Miki D, Imamura M, Ochi H, Kamatani N, Nakamura Y, Chayama K
  • Journal Title: Gut Volume: 60 Pages: 261-267
  • DOI: doi:10.1136/gut.2010.223495.
  • Peer Reviewed
• [Journal Article] A single nucleotidepoly morphism in activated Cdc42 associated tyrosine kinase 1influences the interferon therapy in hepatitis C patients (2011)
  • Author(s): Fujimoto Y, Ochi H, Maekawa T, Abe H, Hayes CN, Kumada H, Nakamura Y, Chayama K
  • Journal Title: J Hepatol Volume: 54 Pages: 629-639
  • DOI: doi:10.1016/j.jhep.2010.07.021.
  • Peer Reviewed
• [Journal Article] IL28B butnot ITPA polymorphism is predictive ofresponse to pegylated interferon, ribavirin, and telaprevir tripletherapy in patients with genotype 1hepatitis C (2011)
  • Author(s): Chayama K, Hayes CN, Abe H, Miki D, Ochi H, Karino Y, Toyota J, NakamuraY, Kamatani N, Sezaki H, Kobayashi M, Akuta N, Suzuki F, Kumada H
  • Journal Title: J Infect Dis Volume: 204 Pages: 84-93
  • DOI: doi:10.1093/infdis/jir210.
  • Peer Reviewed
• [Journal Article] ME3738 enhances the effect of interferon andinhibits hepatitis C virusreplication both in vitro and in vivo (2011)
  • Author(s): Abe H, Imamura M, Hiraga N, Tsuge M, Mitsui F, Kawaoka T, Takahashi S, OchiH, Maekawa T, Hayes CN, Tateno C, Yoshizato K, Murakami S, Yamashita N, Matsuhira T, Asai K, Chayama K
  • Journal Title: J Hepatol Volume: 55 Pages: 11-18
  • DOI: doi:10.1016/j.jhep.2010.10.017.
  • Peer Reviewed
• [Journal Article] IL28 variationaffects expression of interferonstimulated genes and peg-interferonand ribavirin the rapy (2011)
  • Author(s): Abe H, Hayes CN, Ochi H, Maekawa T, Tsuge M, Miki D, Mitsui F, Hiraga N, Imamura M, Takahashi S, Kubo M, Nakamura Y, Chayama K
  • Journal Title: J Hepatol Volume: 54 Pages: 1094-1101
  • DOI: doi:10.1016/j.jhep.2010.09.019.
  • Peer Reviewed
• [Journal Article] Long-term use ofentecavir in nucleoside-naiveJapanese patients with chronichepatitis B infection (2010)
  • Author(s): Yokosuka O, Takaguchi K, Fujioka S, Shindo M, Chayama K, Kobashi H, Hayashi N, Sato C, Kiyosawa K, Tanikawa K, Ishikawa H, Masaki N, Seriu T, Omata M
  • Journal Title: J Hepatol Volume: 52 Pages: 791-799
  • DOI: doi:10.1016/j.jhep.2009.12.036.
  • Peer Reviewed
• [Journal Article] HBx protein is indispensable for development of viraemia in human hepatocyte chimericmice (2010)
  • Author(s): Tsuge M, Hiraga N, Akiyama R, TanakaS, Matsushita M, Mitsui F, Abe H, Kitamura S, Hatakeyama T, Kimura T, Miki D, Mori N, Imamura M, TakahashiS, Hayes CN, Chayama K
  • Journal Title: J Gen Virol Volume: 91 Pages: 1854-1864
  • DOI: doi:10.1099/vir.0.019224-0.
  • Peer Reviewed
• [Journal Article] IL-6-mediated intersubgenotypic variation of interferon sensitivity in hepatitis C virus genotype 2a/ 2b chimeric clones (2010)
  • Author(s): Suda G, Sakamoto N, Itsui Y, NakagawaM, Tasaka-Fujita M, Funaoka Y, Watanabe T, Nitta S, Kiyohashi K, Azuma S, Kakinuma S, Tsuchiya K, Imamura M, Hiraga N, Chayama K, Watanabe M
  • Journal Title: Virology Volume: 407 Pages: 80-90
  • DOI: doi:10.1016/j.virol.2010.07.041.
  • Peer Reviewed
• [Journal Article] ITPA polymorphism affects ribavirin-induced anemia and outcomes of therapy-a genome-wide study ofJapanese HCV virus patients (2010)
  • Author(s): Ochi H, Maekawa T, Abe H, Hayashida Y, Nakano R, Kubo M, Tsunoda T, Hayes CN, Kumada H, Nakamura Y, Chayama K
  • Journal Title: Gastroenterology Volume: 139 Pages: 1190-1197
  • DOI: doi:10.1053/j.gastro.2010.06.071.
  • Peer Reviewed
• [Journal Article] Cellculture and in vivo analyses of cytopathic hepatitis C virus mutants (2010)
  • Author(s): Mishima K, Sakamoto N, Sekine-OsajimaY, Nakagawa M, Itsui Y, Azuma S, Kakinuma S, Kiyohashi K, Kitazume A, Tsuchiya K, Imamura M, Hiraga N, Chayama K, Wakita T, Watanabe M
  • Journal Title: Virology Volume: 405 Pages: 361-369
  • DOI: doi:10.1016/j.virol.2010.06.020.
  • Peer Reviewed
• [Journal Article] Practical evaluation of a mouse with chimeric human liver model for hepatitis C virus infection using an NS3-4A protease inhibitor (2010)
  • Author(s): Kamiya N, Iwao E, Hiraga N, Tsuge M, Imamura M, Takahashi S, Miyoshi S, Tateno C, Yoshizato K, Chayama K
  • Journal Title: J Gen Virol Volume: 91 Pages: 1668-1677
  • DOI: doi:10.1099/vir.0.019315-0.
  • Peer Reviewed
• [Journal Article] Amino acidsubstitution in hepatitis C virus core region and genetic variation near the interleukin 28B gene predict viral response to telaprevir with peginterferon and ribavirin (2010)
  • Author(s): Akuta N, Suzuki F, Hirakawa M, Kawamura Y, Yatsuji H, Sezaki H, Suzuki Y, Hosaka T, Kobayashi M, Saitoh S, Arase Y, Ikeda K, Chayama K, Nakamura Y, Kumada H
  • Journal Title: Hepatology Volume: 52 Pages: 421-4293
  • DOI: doi:10.1002/hep.23690.
  • Peer Reviewed
• [Journal Article] Common variation of IL28 affects gamma-GTP levels and inflammation of the liver in chronically infected hepatitis C virus patients (2010)
  • Author(s): Abe H, Ochi H, Maekawa T, Hayes CN, Tsuge M, Miki D, Mitsui F, Hiraga N, Imamura M, Takahashi S, Ohishi W, Arihiro K, Kubo M, Nakamura Y, ChayamaK
  • Journal Title: J Hepatol Volume: 53 Pages: 439-443
  • DOI: doi:10.1016/j.jhep.2010.03.022.
  • Peer Reviewed
• [Journal Article] A polymorphism in MAPKAPK3 affects response to interferon therapy for chronic hepatitis C (2009)
  • Author(s): Tsukada H, Ochi H, Maekawa T, Abe H, Fujimoto Y, Tsuge M, Takahashi H, Kumada H, Kamatani N, Nakamura Y, Chayama K
  • Journal Title: Gastroenterology Volume: 136 Pages: 1796-1805e1796
  • DOI: doi:10.1053/j.gastro.2009.01.061.
  • Peer Reviewed
• [Journal Article] Adoptive immunotherapy with liver allograft-derived lymphocytes induces anti-HCV activity after liver transplantation in humans and humanized mice (2009)
  • Author(s): Ohira M, Ishiyama K, Tanaka Y, DoskaliM, Igarashi Y, Tashiro H, Hiraga N, Imamura M, Sakamoto N, Asahara T, Chayama K, Ohdan H
  • Journal Title: J Clin Invest Volume: 119 Pages: 3226-3235
  • DOI: doi:10.1172/JCI38374.
  • Peer Reviewed
• [Journal Article] G-to-A hypermutation in hepatitis B virus(HBV) and clinical course of patients with chronic HBV infection (2009)
  • Author(s): Noguchi C, Imamura M, Tsuge M, HiragaN, Mori N, Miki D, Kimura T, TakahashiS, Fujimoto Y, Ochi H, Abe H, MaekawaT, Tateno C, Yoshizato K, Chayama K
  • Journal Title: J Infect Dis Volume: 199 Pages: 1599-1607
  • DOI: doi:10.1086/598951.
  • Peer Reviewed
• [Journal Article] Deletion of angiotensin II type I receptor reduces hepatic steatosis (2009)
  • Author(s): Nabeshima Y, Tazuma S, Kanno K, HyogoH, Chayama K
  • Journal Title: J Hepatol Volume: 50 Pages: 1226-1235
  • DOI: doi:10.1016/j.jhep.2009.01.018.
  • Peer Reviewed
• [Journal Article] Amphipathic DNA polymers inhibit hepatitis C virus infection by blocking viral entry (2009)
  • Author(s): Matsumura T, Hu Z, Kato T, Dreux M, Zhang YY, Imamura M, Hiraga N, JuteauJM, Cosset FL, Chayama K, Vaillant A, Liang TJ
  • Journal Title: Gastroenterology Volume: 137 Pages: 673-681
  • DOI: doi:10.1053/j.gastro.2009.04.048.
  • Peer Reviewed
• [Journal Article] Amphipathic DNA polymers inhibit hepatitis C virus infection by blocking viral entry (2009)
  • Author(s): Kamatani Y, Wattanapokayakit S, OchiH, Kawaguchi T, Takahashi A, Hosono N, Kubo M, Tsunoda T, Kamatani N, KumadaH, Puseenam A, Sura T, Daigo Y, ChayamaK, Chantratita W, Nakamura Y, MatsudaK
  • Journal Title: Nat Genet Volume: 41 Pages: 591-595
  • DOI: doi:10.1038/ng.348.
  • Peer Reviewed
• [Journal Article] Absence of viral interference and different susceptibility to interferon between hepatitis B virus and hepatitis C virus in human hepatocyte chimeric mice (2009)
  • Author(s): Hiraga N, Imamura M, Hatakeyama T, Kitamura S, Mitsui F, Tanaka S, TsugeM, Takahashi S, Abe H, Maekawa T, OchiH, Tateno C, Yoshizato K, Wakita T, Chayama K
  • Journal Title: J Hepatol Volume: 51 Pages: 1045-1054
  • DOI: doi:10.1016/j.jhep.2009.09.002.
  • Peer Reviewed
• [Presentation] Dual Oral Combination Therapy with the NS5A Inhibitor BMS-790052 and the NS3 Protease Inhibitor BMS-650032 Achieved 90% Sustained Virologic Response(SVR12) in HCV Genotype 1b-Infected Null Responders (2011)
  • Author(s): Chayama Kazuaki
  • Organizer: The 62nd Annual Meeting ofAmerican Association for the Study ofLiver Diseases(AASLD)
  • Place of Presentation: SanFrancisco U. S. A
  • Year and Date: 2011-11-07
• [Remarks] ホームページ
  • URL: http://home.hiroshima-u.ac.jp/naika1/