2011 Fiscal Year Final Research Report
Development of a novel"Seed and Soil"cardiac regenerative strategy with ES/iPS cells
| Project/Area Number |
21390239
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Circulatory organs internal medicine
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| Research Institution | Kyoto University |
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Principal Investigator |
YAMASHITA Jun 京都大学, 再生医科学研究所, 准教授 (50335288)
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| Project Period (FY) |
2009 – 2011
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| Keywords | ES細胞 / iPS細胞 / 心臓再生 / 心筋分化 / サイクロスポリンA / 細胞シート |
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| Research Abstract |
In this study, we tried to develop a new efficient cardiac regenerative strategy with a combinatory exploration of transplantable cell sources(seeds) and efficient differentiation environments(soil). We succeeded in inducing cardiomyocytes from human iPS cells using cyclospolin-A(PLoS One, 2011a), developing an efficient differentiation method and purification method for cardiomyocytes from human iPS cells(PLoS One, 2011b). We also showed the potentials and mechanisms of cardiac tissue sheet transplantation in cardiac function after myocardial infarction. Transplantation of the cardiac tissue sheet to a rat myocardial infarction model showed significant and sustained improvement of systolic function accompanied by neovascularization(Stem Cells, 2012). Currently, we identified a novel small compound from marine sponge showing potent cardiomyocyte inducing effect. Thus, we succeeded in establishing a technological basis for"Seed and soil"cardiac regenerative strategy.
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[Journal Article] Pluripotent stem cell-engineered cell sheets re-assembled with defined cardiovascular populations ameliorate reduction in infarct heart function with cardiomyocyte-mediated neovascularization2012
Author(s)
Masumoto H, Matsuo T, Yamamizu K, Uosaki H, Narazaki G, Katayama S, Marui A, Shimizu T, Ikeda T, Okano T, Sakata R, Yamashita JK
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Journal Title
Stem Cells
Volume: 30
Pages: 1196-1205
DOI
Peer Reviewed
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[Journal Article] Induction and enhancement of cardiac cell differentiation from mouse and human induced pluripotent stem cells with cyclosporin-A2011
Author(s)
Fujiwara M, Yan P, Otsuji TG, Narazaki G, Uosaki H, Fukushima H, Kuwahara K, Harada M, Matsuda H, Matsuoka S, Okita K, Takahashi K, Nakagawa M, Ikeda T, Sakata R, Mummery CL, Nakatsuji N, Yamanaka S, Nakao K, Yamashita JK
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Journal Title
PLoS One
Volume: 6
Pages: e16734
URL
Peer Reviewed
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[Journal Article] Therapeutic angiogenesis by transplantation of induced pluripotent stem cell-derived Flk-1 positive cells2010
Author(s)
Suzuki H, Shibata R, Kito T, Ishii M, Li P, Yoshikai T, Nishio N, Ito S, Numaguchi Y, Yamashita JK, Murohara T, Isobe K
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Journal Title
BMC Cell Biol
Volume: 11
Pages: 72
URL
Peer Reviewed
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[Journal Article] The cardiac pacemaker-specific channel Hcn4 is a direct transcriptional target of MEF22009
Author(s)
Kuratomi S, Ohmori Y, Ito M, Shimazaki K, Muramatsu S, Mizukami H, Uosaki H, Yamashita JK, Arai Y, Kuwahara K, Takano M
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Journal Title
Cardiovasc Res
Volume: 83
Pages: 682-687
URL
Peer Reviewed
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