2011 Fiscal Year Final Research Report
Establishment of Molecular Therapy for Severe Heart Failure and Intractable Fetal Arrhythmia
| Project/Area Number |
21390241
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Circulatory organs internal medicine
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| Research Institution | Yamaguchi University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
IKEDA Yasuhiro 山口大学, 大学院・医学系研究科, 助教 (00260349)
YANO Masafumi 山口大学, 医学部附属病院, 講師 (90294628)
YAMAMOTO Takeshi 山口大学, 大学院・医学系研究科, 助教 (50363122)
UEYAMA Tsuyoshi 山口大学, 医学部附属病院, 助教 (60380010)
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| Research Collaborator |
宮崎 要介 山口大学, 大学院, 学生
青山 英和 山口大学, 大学院, 学生
白石 宏造 山口大学, 大学院, 学生
西野 静香 山口大学, 技術, 補佐員
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| Project Period (FY) |
2009 – 2011
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| Keywords | protein phosphatase 1β / adeno-associated virus vector / gene therapy / RNAi / B-type natriuretic peptide promoter |
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| Research Abstract |
The targeting of Ca^<2+> cycling has emerged as a potential therapy for the treatment of severe heart failure. Here, we developed a novel heart-failure-inducible gene transfer system that inhibits PP1 by adeno-associated virus vector 9(AAV9)-mediated shRNA. AAV9 mediated gene transfer mitigated progressive left ventricular remodeling and ameliorated heart failure progression in muscle LIM proteindeficient(MLPKO) mice cardiomyopathy. Heart failure inducible gene therapy system provides new potential as a strategy of treatment of severe heart failure and intractable fetal arrhythmia.
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Research Products
(28 results)
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[Journal Article] Heart Failure-Inducible Gene Therapy Targeting Protein Phosphatase 1 Prevents Progressive Left Ventricular2012
Author(s)
Miyazaki Y, Ikeda Y, Shiraishi K, Fujimoto N. S., Aoyama H, Yoshimura K, Inui M, Hoshijima M, Kasahara H, Aoki H, and Matsuzaki. M
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Journal Title
Remodeling PLoS ONE
Volume: 7(4)
Pages: e35875
Peer Reviewed
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[Journal Article] Mutation-linked defective interdomain interactions within ryanodine receptor cause aberrant Ca channel release leading to catecholaminergic polymorphic ventricular tachycardia2011
Author(s)
Suetomi T, Yano M, Uchinoumi H, Fukuda M, Hino A, Ono M, Xu X, Tateishi H, Okuda S, Doi M, Kobayashi S, Ikeda Y, Yamamoto T, Ikemoto N, Matsuzaki M
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Journal Title
Circulation
Volume: 124(6)
Pages: 682-94
Peer Reviewed
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[Journal Article] TRPC3-mediated Ca2+ influx contributes to Rac1-mediated production of reactive oxygen species in MLP-deficient mouse hearts2011
Author(s)
Kitajima N, Watanabe K, Morimoto S, Sato Y, Kiyonaka S, Hoshijima M, Ikeda Y, Nakaya M, Ide T, Mori Y, Kurose H
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Journal Title
Biochem Biophys Res Commun
Volume: 409(1)
Pages: 108-13
Peer Reviewed
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[Journal Article] Catecholaminergic polymorphic ventricular tachycardia is caused by mutation-linked defective conformational regulation of the ryanodine receptor2010
Author(s)
Uchinoumi H, Yano M, Suetomi T, Ono M, Xu X, Tateishi H, Oda T, Okuda S, Doi M, Kobayashi S, Yamamoto T, Ikeda Y, Ohkusa T, Ikemoto N, Matsuzaki M
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Journal Title
Circ Res
Volume: 106(8)
Pages: 1413-24
Peer Reviewed
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[Journal Article] Defective calmodulin binding to the cardiac ryanodine receptor plays a key role in CPVT-associated channel dysfunction2010
Author(s)
Xu X, Yano M, Uchinoumi H, Hino A, Suetomi T, Ono M, Tateishi H, Oda T, Okuda S, Doi M, Kobayashi S, Yamamoto T, Ikeda Y, Ikemoto N, Matsuzaki M
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Journal Title
Biochem Biophys Res Commun
Volume: 394(3)
Pages: 660-6
Peer Reviewed
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[Presentation] Isoform-specific role of protein phosphatase 1 catalytic subunits in sarcoplasmic reticulum-mediated ca2+ cycling2010
Author(s)
Ikeda Y, Aoyama H, Miyazaki Y, Shiraishi K, Yoshimura K, Nishino S, Yamamoto T, Yano M, Inui M, Aoki H, Matsuzaki M
Organizer
19th International Conference of the Cardiovascular System Dynamics Society
Place of Presentation
Fukuoka, Centennial Hall Kyushu University School of Medicine
Year and Date
20100923-26
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