Molecular mechanism of the heart by the autonomic nervous system

2012 Fiscal Year Final Research Report

• Project/Area Number: 21390246
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Circulatory organs internal medicine
• Research Institution: Yokohama City University
• Principal Investigator: ISHIKAWA Yoshihiro 横浜市立大学, 医学研究科, 教授 (40305470)
• Co-Investigator(Kenkyū-buntansha): SATO Motohiko 愛知医科大学, 医学部, 教授 (40292122) ; OKUMURA Satoshi 鶴見大学, 歯学部, 教授 (60233475)
• Project Period (FY): 2009 – 2012
• Keywords: 自律神経 / 心機能制御 / cAMP / カテコラミン / 分子

Research Abstract

Adenylyl cyclase is a membrane bound enzyme that catalyzes the production of cAMP. Epac is a new molecule, which is regulated by cAMP independently from PKA. In the model of Epac overexpression, we found an interesting cross talk between cytokine and cAMP signal.

Research Products

• [Journal Article] Secretoglobin 3A2 suppresses pulmonary fibrosis by TGFbeta signaling down-regulation. (2011)
  • Author(s): Kurotani R, Okumura S, Matsubara T, Yokoyama U, Buckley JR, Tomita T, Kezuka K, Nagano T, Esposito D, Taylor TE, Gillette WK, Ishikawa Y, Abe H, Ward JM, and Kimura S
  • Journal Title: J. Biol. Chem Volume: 286 Pages: 19682-19692
  • Peer Reviewed
• [Journal Article] Identification of transcription factor E3(TFE3) as areceptor-independent activator of G α 16: Gene regulation by nuclear Gα subunit and its activato (2011)
  • Author(s): Sato M, Hiraoka M, Suzuki H, Bai Y, Kurotani R, Yokoyama U, Okumura S, Cismowski MJ, Lanier SM, and Ishikawa Y
  • Journal Title: JBiol Chem Volume: 286 Pages: 17766-17776
  • Peer Reviewed
• [Journal Article] Apoptosis in heart failure.-The role of the beta-adrenergic receptor-mediated signaling pathway and p53-mediated signaling pathway in the poptosis of cardio-myocytes (2011)
  • Author(s): Fujita T, Ishikawa Y
  • Journal Title: Circ J Volume: 75 Pages: 1811-1818
  • Peer Reviewed
• [Journal Article] The roles of cytochrome P450 in ischemic heart disease (2011)
  • Author(s): Sato M, Yokoyama U, Fujita T, Okumura S,and Ishikawa Y
  • Journal Title: Curr DrugMetab Volume: 12 Pages: 526-32
  • Peer Reviewed
• [Journal Article] Accesory proteins for heterotrimeric G protein: Implication in the cardiovascularsystem (2010)
  • Author(s): Sato M, Ishikawa Y
  • Journal Title: Pathophysiology Volume: 17 Pages: 89-99
  • Peer Reviewed
• [Journal Article] Differentialregulation of vascular tone and remodeling via stimulation of type 2 and type 6 adenylyl cyclases in the ductus arteriosus (2010)
  • Author(s): Yokoyama U, Minamisawa S, Katayama A, Tang T, Suzuki S, Iwatsubo K, Iwasaki S, Kurotani R, Okumura S, Sato M, Yokota S, Hammond HK, and Ishikawa Y
  • Journal Title: Circ. Res Volume: 106 Pages: 1882-1892
  • Peer Reviewed
• [Journal Article] Differential roles of Epac in regulating cell death in neuronal and myocardial cells (2010)
  • Author(s): 1Suzuki S, Yokoyama U, Abe T, KiyonariH,Yamashita N, Kato Y, Kurotani R, Sato M,Okumura S, and Ishikawa Y
  • Journal Title: J.Biol Chem Volume: 285 Pages: 24248-24259
  • Peer Reviewed
• [Journal Article] Effect of cardiac overexpression of type 6 adenylyl cyclase affectson the response to chronic pressure overload (2010)
  • Author(s): Guellich A, Vatner DE, Gao S, Hong C, Yan L, Wagner TE, Dhar S, Ghalech B, Hittinger L, Iwatsubo K, Ishikawa Y, and Vatner SF
  • Journal Title: Am J Physiol Heart Circ Physiol Volume: 299 Pages: 707-712
  • Peer Reviewed
• [Journal Article] Epac Increases Melanoma cell migration by Heparan Sulfate-Related Mechanism (2009)
  • Author(s): Baljinnyam E, Iwatsubo K, Kurotani R,Wang X, Ulucan C, Iwatsubo M, LagunoffD, and Ishikawa Y
  • Journal Title: Am. J. Physiol Volume: 297 Pages: 802-813
  • Peer Reviewed
• [Journal Article] Activator of G protein signaling 8 (AGS8) is required for hypoxia-induced apoptosis of cardio-myocytes: role of G betagamma and connexin 43(CX43) (2009)
  • Author(s): Sato M, Jiao Q, Honda T, Kurotani R, Toyota E, Okumura S, Takeya T, MinamisawaS,Lanier SM, and Ishikawa Y
  • Journal Title: J. Biol. Chem Volume: 284 Pages: 31431-31440
  • Peer Reviewed
• [Presentation] 心臓型アデニル酸シクラーゼ選択的抑制薬を用いた新しい心不全治療 (2011)
  • Author(s): 奥村 敏, 川又史佳, 金慧玲, 蔡 文倩, 金美花, 日高祐子, 吹田憲治, 岩坪耕策, 石川義弘
  • Organizer: 第6回高血圧と冠動脈疾患研究会
  • Place of Presentation: 大手町サンケイプラザ(東京都)
  • Year and Date: 2011-12-23
• [Presentation] Epac activation protects heart from interleukin-6-induced cardiac dysfunction by inhibiting STAT/iNOS signaling (2011)
  • Author(s): Okumura S, Jin M, Kawamata F, Jin H-L, Cai W, Bai Y, Suita K, Hidaka Y, Tsunematsu T, Ishikawa Y
  • Organizer: The 85thAmerican Heart Association
  • Place of Presentation: Orlando. USA
  • Year and Date: 2011-11-16
• [Presentation] Cardiac protection from hypoxic injury by a novel synthetic peptide targeting the activator of G-protein signaling 8-mediated G βγsignal (2011)
  • Author(s): Sato M, Hiraoka M, Yamane Y, Feng X, Ishikawa Y
  • Organizer: The 85thAmerican Heart Association
  • Place of Presentation: Orlando, USA
  • Year and Date: 2011-11-16
• [Presentation] pac1 Deficiency Inhibit Neointima Formation after Vascular Injury in vivo (2011)
  • Author(s): Kato Y, Yokoyama U, Okumura S, Minamisawa S,Sata M,Miyajima E, Ishikawa Y
  • Organizer: The 85thAmerican Heart Association
  • Place of Presentation: Orlando, USA
  • Year and Date: 2011-11-16
• [Presentation] Epac1 は細胞極性を促進し内膜肥厚形成に関与する (2011)
  • Author(s): 加藤優子, 横山詩子, 奥村敏, 南沢享,佐田政隆, 宮島栄治, 石川義弘
  • Organizer: The 2ndMolecular Cardiovascular Conference II
  • Place of Presentation: キロロ ホテルピアノ(北海道)
  • Year and Date: 2011-09-04
• [Presentation] Epac1は血管障害時の内膜肥厚形成を促進する (2011)
  • Author(s): 加藤 優子, 横山 詩子, 奥村 敏, 南沢 享, 佐田 政隆, 宮島 栄治, 石川 義弘
  • Organizer: 第53回日本平滑筋学会
  • Place of Presentation: 第53回日本平滑筋学会
  • Year and Date: 2011-08-04
• [Presentation] Disruption of Epac1 Protects Heart from Catecholamine-mediated Cardiac Fibrosis (2011)
  • Author(s): Okumura S, Cai W, Jin M, Bai Y, Tsunematsu T, HidakaY, Kurotani R,Ishikawa Y
  • Organizer: 第75回日本循環器学会学術集会
  • Place of Presentation: パシフィコ横浜(神奈川県)
  • Year and Date: 2011-08-04
• [Presentation] 2010 2ndInternational Congress of Cardiology,Shanghai Heart Failure (2010)
  • Author(s): Yoshihiro Ishikawa: Targeting Adenylyl Cyclase Isoforms in Treating Heart Failure
  • Organizer: Left Ventricular Disfunction and Preserved Ejection Fraction(招待講演)
  • Place of Presentation: Shanghai, China
  • Year and Date: 2010-12-09
• [Presentation] Magnetic anti-cancer drug for hyperthermal and chemotherapy. (2010)
  • Author(s): Yoshihiro Ishikawa
  • Organizer: The 8thInternathional Drug Discovery Scienceand Technology oncology and Drug Discovery
  • Place of Presentation: Beijing, China
  • Year and Date: 2010-10-26
• [Presentation] 心不全と交感神経の分子メカニズム (2010)
  • Author(s): 石川義弘
  • Organizer: 第33回日本高血圧学会(招待講演)
  • Place of Presentation: 福岡国際会議場(福岡県)
  • Year and Date: 2010-10-17
• [Presentation] Counteracting regulation of vascular remodeling via stimulation oftype 2 and type 6 adenylyl cy clases in the ductus arteriosus (2010)
  • Author(s): Minamisawa S, Yokoyama U, Akaike T, Hammond K, Ishikawa Y
  • Organizer: The 16th International Vascular Biology Meeting
  • Place of Presentation: Los Angeles, USA
  • Year and Date: 2010-06-24
• [Presentation] Disruption of Epac1 genepreserves cardiac function against pressureoverload and chronic catecholamine stress (2010)
  • Author(s): Jin MH, Okumura S, Cai W, Hidaka Y, Ishikawa Y
  • Organizer: The 87thAnnual Meeting of the PhysiologicalSociety of Japan
  • Place of Presentation: いわて県民情報交流センター(岩手県)
  • Year and Date: 2010-05-21
• [Presentation] Epac1 Protects Heart From Lipopolysaccharide-induced Cardiac Dysfunction By Inhibiting The Toll-likeR eceptor4 Signaling Pathway (2009)
  • Author(s): Okumura S, Jin M, Bai Y, Suzuki S, XuanX, Cai W, Hidaka Y, Kurotani R, Yokoyama U, Ishikawa Y
  • Organizer: AmericanHeart Association Scientific Sessions
  • Place of Presentation: Orlando, USA
  • Year and Date: 2009-11-18
• [Presentation] Adenylyl Cyclase Type2 and 6 DifferentiallyPromote VascularTone and Remodeling in Ductus Arteriosus.36th International Congress of PhysiologicalSciences (2009)
  • Author(s): Katayama A, Yokoyama U, Tang T, AkaikeT, Hammond HK, Minamisawa S, Ishikawa Y
  • Organizer: 36th International Congress of PhysiologicalSciences
  • Place of Presentation: 京都国際会議場(京都府)
  • Year and Date: 2009-08-01
• [Book] ボロン・ブールペープ (2011)
  • Author(s): 総監訳 泉井亮,監訳 河南洋, 久保川学, 訳 青木史暁, 赤池紀生, 石川義弘, 石山延吉, 上田陽一, 上野伸哉, 大野忠雄, 河原克雅, 河南洋, 北村竜一, 久保川学, 桑木共之,小島至, 佐々木和彦, 高瀬堅吉, 槌田成紀, 照井直人, 中村晃, 花森隆充, 福田康一郎,藤原広明, 船橋利也, 山崎将生, 山田聡子, 山本頼綱, 泉井亮
  • Total Pages: 14-24 章
  • Publisher: 西村書店
• [Patent(Industrial Property Rights)] ビダラビンによる心房細動治療 (2011)
  • Inventor(s): 石川義弘 奥村敏、吹田憲治
  • Industrial Property Rights Holder: 横浜市立大学
  • Industrial Property Number: PCT/JP2012/069540
  • Filing Date: 2011-10-07
  • Overseas
• [Patent(Industrial Property Rights)] ビダラビンによる心房細動治療 (2011)
  • Inventor(s): 石川義弘 奥村敏、吹田憲治
  • Industrial Property Rights Holder: 横浜市立大学
  • Industrial Property Number: 特願 2011-222421
  • Filing Date: 2011-10-07
• [Patent(Industrial Property Rights)] アデニル酸シクラーゼの活性調整剤 (2010)
  • Inventor(s): 石川義弘、奥村敏、星野雄二郎、井上誠一
  • Industrial Property Rights Holder: 横浜市立大学・横浜国立大学
  • Industrial Property Number: 特願 2010-240301
  • Filing Date: 2010-10-27
• [Patent(Industrial Property Rights)] アデニル酸シクラーゼの活性調節剤 (2010)
  • Inventor(s): 石川義弘、奥村敏、星野雄二郎、井上誠一
  • Industrial Property Rights Holder: 横浜市立大学・横浜国立大学
  • Industrial Property Number: PCT/JP2011/74098
  • Filing Date: 2010-10-20
  • Overseas