Cardiomyocyte niche for cardiac regeneration:

2011 Fiscal Year Final Research Report

• Project/Area Number: 21390248
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Circulatory organs internal medicine
• Research Institution: Keio University
• Principal Investigator: MAKINO Shinji 慶應義塾大学, 医学部, 特任准教授 (20306707)
• Co-Investigator(Kenkyū-buntansha): SANO Motoaki 慶應義塾大学, 医学部, 講師 (30265798)
• Project Period (FY): 2009 – 2011
• Keywords: めだか / 順遺伝学 / プロテオグリカン

Research Abstract

Ventricular chamber growth requires cardiomyocyte proliferation and progenitor cells migration from outside the heart tube. We investigated how these progenitors are integrated into heart.Using forward-genetic ENU mutagenesis screening, we identified a ca

Research Products

• [Journal Article] Wnt2 accelerates cardiac myocyte differentiation from ES-cell derived mesodermal cells via non-canonical pathway. (2011)
  • Author(s): Onizuka T, Yuasa S, Kusumoto D, Shimoji K, Egashira T, Ohno Y, Kageyama T, Tanaka T, Hattori F, Fujita J, Ieda M, Kimura K, Makino S, Sano M, Kudo A, Fukuda K
  • Journal Title: J Mol Cell Cardiol.
  • Peer Reviewed
• [Journal Article] G-CSF influences mouse skeletal muscle development and regeneration by stimulating myoblast proliferation (2011)
  • Author(s): Hara M, YuasaS, Shimoji K, Onizuka T, Hayashiji N, Ohno Y, Arai T, Hattori F, Kaneda R, Kimura K, Makino S, Sano M, Fukuda K
  • Journal Title: The Journal of Experimental Medicine Volume: 208(4) Pages: 715-27
  • Peer Reviewed
• [Journal Article] Simple autogeneic feeder cell preparation for pluripotent stem cells (2011)
  • Author(s): Li W, Yamashita H, Hattori F, Chen H, Tohyama S, Satoh Y, Sasaki E, Yuasa S, Makino S, Sano M, Fukuda K
  • Journal Title: Stem Cell Res. Volume: 6(1) Pages: 83-9
  • Peer Reviewed
• [Journal Article] Neural crest-derived stem cells migrate and differentiate into cardiomyocytes after myocardial infarction (2011)
  • Author(s): Tamura Y, Matsumura K, Sano M, Tabata H, Kimura K, Ieda M, Arai T, Ohno Y, Kanazawa H, Yuasa S, Kaneda K, Makino S, Nakajima K, Okano H, and Fukuda K.
  • Journal Title: Arterioscler Thromb Vasc Biol.
  • Peer Reviewed
• [Journal Article] Gene knock-outs of inositol 1,4,5-trisphosphate receptors types 1 and 2 result in perturbation of cardiogenesis. (2010)
  • Author(s): Uchida K, Aramaki M, Nakazawa M, Yamagishi C, Makino S, Fukuda K, Nakamura T,Takahashi T, Mikoshiba K, Yamagishi H
  • Journal Title: PLoS ONE Volume: 5(9) Pages: e12500
  • Peer Reviewed
• [Journal Article] Zac1 is an essential transcription factor for cardiac morphogenesis. (2010)
  • Author(s): Yuasa S, Onizuka T, Shimoji K, Ohno Y, Kageyama T, Yoon SH, Egashira T, Seki T, Hashimoto H, Nishiyama T, Kaneda R, Murata M, Hattori F, Makino S, Sano M, Ogawa S, Prall OW, Harvey RP, Fukuda K
  • Journal Title: Circ Res. Volume: 106(6) Pages: 1083-91
  • Peer Reviewed
• [Journal Article] Epigenetic control of cardiomyocyte production in response to a stress during the medaka heart development (2010)
  • Author(s): Taneda Y, Konno S, Makino S, Morioka M, Fukuda K, Imai Y, Kudo A, Kawakami A
  • Journal Title: Dev. Biol. Volume: 21 Pages: 30-40
  • Peer Reviewed
• [Journal Article] Nongenetic method for purifying stem cell-derived cardiomyocytes (2010)
  • Author(s): Hattori F, Chen H, Yamashita H, Tohyama S, Satoh Y, Yuasa S, Li W, Yamakawa H, Tanaka T, Onitsuka T, Shimoji K, Ohno Y, Egashira T, Kaneda R, Murata M, Hidaka K, Morisaki T, Sasaki E, Suzuki T, Sano M, Makino S, Oikawa S & Fukuda K
  • Journal Title: Nature methods Volume: 7(1) Pages: 61-6
  • Peer Reviewed
• [Journal Article] Omentopexy enhances graft function in myocardial cell sheet transplantation. (2009)
  • Author(s): Suzuki R, Hattori F, Itabashi Y, Yoshioka M, Yuasa S, Manabe-Kawaguchi H, Murata M, Makino S, Kokaji K, Yozu R, Fukuda K
  • Journal Title: Biophys Res Commun. Volume: 387(2) Pages: 353-9
  • Peer Reviewed
• [Presentation] ncreased Binding Between Titin and Murf1 is Associated with Hypertrophic Cardiomyopathy in a Fish Model and In Human Patients (2011)
  • Author(s): Enomoto H, Makino S, Higashikuse Y, Fukuda K(4) 第84回アメリカ心臓病学会2011.11.12-16 Orlando Florida U.S.ACell Type-Specific Decrease of Gene Body DNA Methylation Levels in Cardiomyocyte-specific Genes Contribute to the Transcriptional Efficiency at Postnatal Deve
  • Organizer: 第84回アメリカ心臓病学会
  • Place of Presentation: Orlando Florida U.S.A
  • Year and Date: 20111112-16
• [Presentation] Cell Type-Specific Decrease of Gene Body DNA Methylation Levels in Cardiomyocyte-specific Genes Contribute to the Transcriptional Efficiency at Postnatal Development of Mouse Cardiomyocytes (2011)
  • Author(s): Oda M, Makino S, Fukuda K
  • Organizer: 第84回アメリカ心臓病学会
  • Place of Presentation: Orlando Florida U.S.A
  • Year and Date: 20111112-16
• [Presentation] Epigenetic Changes in Myosin Heavy Chain (MHC) Gene Regulation in Mouse Cardiomyocytes (2011)
  • Author(s): Oda M, Makino S, Fukuda K
  • Organizer: 第75回日本循環器学会
  • Place of Presentation: 横浜
  • Year and Date: 20110803-04
• [Presentation] Missense Mutation in the M-band Region of titin Leads to Hypertrophic Cardiomyopathy in Medaka Fish and Human (2011)
  • Author(s): Enomoto H, Makino S, Higashikuse Y, Sano M, Kudo A, Kawakami A, Kimura A, Arimura T, Fukuda K
  • Organizer: 第75回日本循環器学会
  • Place of Presentation: 横浜
  • Year and Date: 20110803-04
• [Presentation] Recruitment of Cardiac Progenitor Cells through Outflow Tract is Essential for Ventricular Chamber Formation (2011)
  • Organizer: 第75回日本循環器学会
  • Place of Presentation: 横浜
  • Year and Date: 20110803-04
• [Presentation] A New Animal Model of Takotsubo Cardiomyopathy by Pilocarpine-induced Epilepsy (2011)
  • Author(s): Arai T, Kimura, K, Makino S, Sano M, Fukuda K
  • Organizer: 第75回日本循環器学会
  • Place of Presentation: 横浜
  • Year and Date: 20110803-04
• [Presentation] Can the heart regenerate? Approaches or understanding developmental cardiac diversity and reconstruction of the functional heartRecruitment of Cardiac Progenitor Cells through Outflow Tract is Essential for Ventricular Chamber Formation in Medaka Fish (2011)
  • Author(s): Makino S, Yoon SH, Fukuda K
  • Organizer: 第34回日本分子生物学会
  • Place of Presentation: 横浜 ワークショップ
  • Year and Date: 2011-12-13
• [Presentation] Alteration of Binding Between Titin and Murf1 is Associated with Hypertrophic Cardiomyopathy in a Fish Model and In Human Familial HCM Patients (2011)
  • Author(s): Shinji Makino
  • Organizer: The 28th Annual Meeting of the International Society for Heart Research
  • Place of Presentation: To kyoSymposium3
  • Year and Date: 2011-12-03
• [Presentation] Symposium7 A comprehensive approach to understand the formation of the cardiovascular networkS07-5 Genetic analysis of ventricular chamber formation (2011)
  • Author(s): Makino S
  • Organizer: 第88回日本生理学会、第116回日本解剖学会、合同総会
  • Place of Presentation: 横浜
  • Year and Date: 2011-03-28
• [Presentation] DNA Methylation Changes in the b-MHC Locus Mediate Gene Regulation by Antisense RNA in Mouse Postnatal Cardiomyocyte Development (2010)
  • Author(s): Oda M, Makino S, Fukuda K
  • Organizer: 第83回アメリカ心臓病学会
  • Place of Presentation: Chicago Illinois U.S.A
  • Year and Date: 20101113-17
• [Presentation] Versican is Essential for Chamber Formation by Recruiting Cardiac Progenitor Cells from the Outflow Tract in Medaka Fish (2010)
  • Author(s): Yoon SH, Makino S,, Fukuda K
  • Organizer: 第83回アメリカ心臓病学会
  • Place of Presentation: Chicago Illinois U.S.A
  • Year and Date: 20101113-17
• [Presentation] Mutation of the C-terminus of titin Leads toHypertrophic Cardiomyopathy in Medaka Fish (2010)
  • Author(s): Makino S, Higashikuse Y, Yoon SH, Sano M, Kudo A, Kawakami A, Fukuda K
  • Organizer: 第17回Weinstein Cardiovascular Development Conference
  • Place of Presentation: Royal Tropical Institute (Tropenmuseum) Amsterdam, The Netherlands
  • Year and Date: 20100520-22
• [Presentation] Versican is Essential for Chamber Formation by Recruitment of Islet-1-Expressing Cardiac Progenitor Cells (2010)
  • Author(s): Yoon SH, Makino S, Fukuda K
  • Organizer: 第74回日本循環器学会
  • Place of Presentation: 京都
  • Year and Date: 20100305-07
• [Presentation] Hematopoetic-miRNAs Regulate Cardiogenesis and Somitegenesis in the Developing Zebrafish Mesoderm (2010)
  • Author(s): Nishiyama T, Kaneda R, Makino S, Fukuda K
  • Organizer: 第74回日本循環器学会
  • Place of Presentation: 京都
  • Year and Date: 20100305-07
• [Presentation] Inhibition of BMP Signaling Results in Efficient Cardiomyocyte Induction through Cellular Subpopulation with DNA Hypomethylation in Cerberus 1 Homolog Gene (2010)
  • Author(s): Oda M, Makino S, Fukuda K
  • Organizer: 第74回日本循環器学会
  • Place of Presentation: 京都
  • Year and Date: 20100305-07
• [Presentation] Dominant Negative Suppression of Rad Leads Intracellular Ca2+ Overload via Up-regulation of Cardiac Ryanodine Receptor Activity (2010)
  • Author(s): Yamakawa H, Murata M, Makino S, Fukuda K
  • Organizer: 第74回日本循環器学会
  • Place of Presentation: 京都
  • Year and Date: 20100305-07
• [Presentation] DNA Methylation of the Fibroblast Growth Factor Receptor 2 Gene Affects the Cardiomyocyte-prone State of Embryonic Stem Cells (2010)
  • Author(s): Oda M, Makino S, Fukuda K
  • Organizer: 第74回日本循環器学会
  • Place of Presentation: 京都
  • Year and Date: 20100305-07
• [Presentation] Mutation of the C-terminus of titin leads to Hypertrophic Cardiomyopathy in the Medaka Fish (2010)
  • Author(s): Higashikuse Y, Makino S, Fukuda K
  • Organizer: 第74回日本循環器学会
  • Place of Presentation: 京都
  • Year and Date: 20100305-07
• [Presentation] ワークショップ: 小型魚類メダカやゼブラフィッシュを用いた疾患研究Disease studies using medaka and zebrafish心臓発生・分化の機序解明から新たな心不全治療開発へ (2010)
  • Author(s): 牧野 伸司
  • Organizer: 第33回日本分子生物学会
  • Place of Presentation: 神戸
  • Year and Date: 2010-12-08
• [Presentation] Mutation around the Titin kinase Leads to Diastolic Dysfunction in Medaka Fish (2010)
  • Author(s): Higashikuse Y, Makino S, Yoon SH, Sano M, Kudo A, Kawakami A, Fukuda K
  • Organizer: 第27回International Society of Heart Research(ISHR)
  • Place of Presentation: Kyoto
  • Year and Date: 2010-05-14
• [Presentation] ゼブラフィッシュMusashi遺伝子の同定Characterization of RNA binding protein Musashi in zebrafish (2009)
  • Author(s): 梅井 正彦、芝田 晋介、牧野 伸司、岡野 栄之
  • Organizer: 第32回日本分子生物学会
  • Place of Presentation: 横浜
  • Year and Date: 20091209-12
• [Presentation] MicroRNA 142-3p Regulates Heart Development, Somitegenesis and Hematopoiesis in the Stage of Early Mesoderm Formation (2009)
  • Author(s): Nishiyama T, Kaneda R, Makino S, Fukuda K
  • Organizer: 第82回アメリカ心臓病学会
  • Place of Presentation: Orlando Florida U.S.A
  • Year and Date: 20091114-18
• [Presentation] ATF4-regulated Metabolic Shift Towards Phgdh-mediated Serine Synthesis Enhances Cardioprotection against Oxidative Stress (2009)
  • Author(s): Endo J, Sano M, Makino S, Fukuda K
  • Organizer: 第82回アメリカ心臓病学会
  • Place of Presentation: Orlando Florida U.S.A
  • Year and Date: 20091114-18
• [Book] Regenerating the Heart (Stem Cells and the Cardiovascular System)Part I Stem Cells for Regeneration of Mechanical Function (Chapter6)"Methods for Differentiation of Bone Marrow Derived Stem Cells into Myocytes" (2011)
  • Author(s): Shinji Makino & Keiichi Fukuda
  • Total Pages: 67-83
  • Publisher: Humana Press Ira S. Cohen & Glenn R. Gaudette Editors
• [Book] Hypoxia-inducible Vascular Endothelial Growth Factor-engineered Mesenchymal Stem Cells Prevent Myocardial Ischemic Injury. (2010)
  • Author(s): Makino S, Fukuda K
  • Publisher: Research Highlights for Personalized Medicine
• [Book] 循環器科, 循環器疾患の再生医療update-現状と展望ー(Cells and genes for cardiac regeneration) (2009)
  • Total Pages: 404-407
  • Publisher: 科学評論社
• [Book] 呼吸と循環 57卷11号 心筋の再生治療 (2009)
  • Total Pages: 1191-1195
  • Publisher: 医学書院