2011 Fiscal Year Final Research Report
Basic and clinical research of viral gastroenteritis in children and its risk evaluation
| Project/Area Number |
21390315
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Pediatrics
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| Research Institution | Nihon University (2011)
Aino University (2009-2010) |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
OKITSU Shoko 東京大学, 医学系研究科, 客員研究員 (10082215)
NISHIO Osamu 愛知医科大学, 医学部, 客員教授 (40270631)
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| Project Period (FY) |
2009 – 2011
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| Keywords | ウイルス / 胃腸炎 / 小児 / 分子疫学 / リスク評価 |
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| Research Abstract |
We have produced new virus-like particles(VLPs) of norovirus and sapovirus in order to update the consequences of viral genetic variability. The immunochromatography(IC) diagnosis test kits have been developed/improved by these new VLPs. In addition to the molecular epidemiological study of rotavirus, norovirus, sapovirus, adenovirus and astrovirus that we have done, the novel diarrheal viruses such as Aichi virus, parechovirus, bocavirus and Saffold virus have also been found in diarrheal stool samples. Murine norovirus was used as a novel surrogate to evaluate disinfectants, heating, and antiviral activity for human norovirus. We challenged multiple-cell cultivation assays for norovirus but did not succeed at this moment. We also examined the binding activities of norovirus and sapovirus to histo-blood group antigens of saliva and intestinal cells. It was interesting to find that norovirus, but not sapovirus, showed high binding activity.
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[Journal Article] Novel human adenovirus strains2012
Author(s)
Matsushima Y, Shimizu H, Kano A, Nakajima E, Ishimaru Y, Dey SK, Watanabe Y, Adachi F, Suzuki K, Mitani K, Fujimoto T, Phan TG, Ushijima H
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Journal Title
Bangladesh. Emerg Infect Dis
Volume: (in press)
DOI
Peer Reviewed
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[Journal Article] Detection and molecular characterization of cosavirus in adults with diarrhea, Thailand2012
Author(s)
Khamrin P, Chaimongkol N, Malasao R, Suantai B, Saikhruang W, Kongsricharoern T, Ukarapol N, Okitsu S, Shimizu H, Hayakawa S, Ushijima H, Maneekarn N
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Journal Title
Virus Genes
Volume: 44(2)
Pages: 244-246
DOI
Peer Reviewed
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[Journal Article] Detection and genetic characterization of rotavirus infections in non-hospitalized children with acute gastroenteritis in Japan, 2007-20092011
Author(s)
Chan-it W, Thongprachum A, Dey SK, Phan TG, Khamrin P, Okitsu S, Nishimura S, Kobayashi M, Kikuta H, Baba T, Yamamoto A, Sugita K, Hashira S, Tajima T, Ishida S, Mizuguchi M. Ushijima H
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Journal Title
Infect Genet Evol
Volume: 11
Pages: 415-422
URL
Peer Reviewed
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[Journal Article] Human parechovirus infection in children hospitalized with acute gastroenterisi in Sri Lanka2011
Author(s)
Pham NTK, Takanashi S, Tran DN, Quang DT, Abeysekera C, Abeygunawardene A, Khamrin P, Okitsu S, Shimizu H, Mizuguchi M, Ushijima H
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Journal Title
J Clin Microbiol
Volume: 49(1)
Pages: 364-366
DOI
Peer Reviewed
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[Journal Article] Detection of human parechovirus in stool samples collected from children with acute gastroenteritis in Japan during 2007-20082011
Author(s)
Pham NT, Chan-it W, Khamrin P, Nishimura S, Kikuta H, Sugita K, Baba T, Yamamoto A, Okitsu S, Mizuguchi M, Ushijima H
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Journal Title
J Med Virol
Volume: 83(2)
Pages: 331-336
Peer Reviewed
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[Journal Article] Genotypic linkage of VP4, VP6, VP7, NSP4, NSP5 genes of rotaviruses circulating among children with acute gastroenteritis in Thailand2010
Author(s)
Khamrin P, Maneekarn N, Malasao R, Nguyen TA, Ishida S, Okitsu S, Ushijima H
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Journal Title
Infect Genet Evol
Volume: 10
Pages: 467-472
DOI
Peer Reviewed
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[Journal Article] Sequence analysis of the VP7 gene of human rotaviruses G2 and G4 isolated in Japan, China, and Vietnam during 2001-20032010
Author(s)
Trinh QD, Pham NTK, Nguyen TA, Phan TG, Yan H, Hoang LP, Khamrin P, Maneekarn N, Li Y, Okitsu S, Mizuguchi M, Ushijima H
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Journal Title
J Med Virol
Volume: 82(5)
Pages: 878-85
DOI
Peer Reviewed
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[Journal Article] Circulating of human bocavirus 1, 2, 3, and 4 in pediatric patients with acute gastroenteritis in Thailand. Infection
Author(s)
Khamrin P, Malasao R, Chaimongkol N, Ukarapol N, Kongsricharoern T, Okitsu S, Hayakawa S, Ushijima H, Maneekarn N
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Journal Title
Genetics and Evolution
Volume: 12
Pages: 565-569
URL
Peer Reviewed
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