2011 Fiscal Year Final Research Report
Establishment of monitoring system with AID-associated microRNA-155 for cutaneous T-cell lymphoma and its application for therapy
| Project/Area Number |
21390328
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Dermatology
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| Research Institution | Hamamatsu University School of Medicine (2010-2011)
University of Occupational and Environmental Health, Japan (2009) |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
NAKAMURA Motonobu 産業医科大学, 医学部, 准教授 (30303837)
SHIMAUCHI Takatoshi 浜松医科大学, 医学部, 講師 (90399204)
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| Project Period (FY) |
2009 – 2011
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| Keywords | 皮膚T細胞リンパ腫 / AID / 成人T細胞白血病/リンパ腫 / マイクロRNA / 菌状息肉症 / セザリー症候群 |
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| Research Abstract |
It has been reported that AID is involved in the development of virus-associated neoplasms. Moreover, microRNA-155 downregulates the expression of AID. On the other hand, point mutations of p53 have been reported in the patients with cutaneous T-cell lymphoma(CTCL) including ATLL. By real-time PCR analysis, we investigated the expression of AID and microRNA-155 in the peripheral blood of patients with mycosis fungoides, Sezary syndrome, and ATLL in a comparison with normal healthy subjects. We found that AID expression was higher in the peripheral blood lymphocytes and tumor-infiltrating cells in CTCL, especially ATLL. We isolated circulating CD4-, CD4+CD25-, and CD4+CD25+cells by MACS and examined the mRNA expression of AID and microRNA-155 in each fraction. In addition, skin specimens and lymph nodes from the patients were also tested. We found that AID expression was elevated with decreased expression of microRNA-155, particularly in ATLL, suggesting that AID and its regulatory microRNA are involved in the development of this virus-associated lymphoma.
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Research Products
(36 results)
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[Journal Article] The type of skin eruptions is an independent prognostic indicator for adult T-cell leukemia/lymphoma2011
Author(s)
Sawada Y, Hino R, Hama K, Ohmori S, Fueki H, Yamada S, Fukamachi S, Tajiri M, Kumo R, Yoshioka M, Nakashima D, Sugita K, Yoshiki R, Shimauchi T, Mori T, Izu K, Kobayashi M, Nakamura M, Tokura Y
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Journal Title
Blood
Volume: 117
Pages: 3961-3967
Peer Reviewed
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[Journal Article] Activated regulatory T cells are the major T cell type emigrating from the skin during a cutaneous immune response in mice2010
Author(s)
Tomura M, Honda T, Tanizaki H, Otsuka A, Egawa G, Tokura Y, Waldmann H, Hori S, Cyster JG, Watanabe T, Miyachi Y, Kanagawa O, Kabashima K
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Journal Title
J Clin Invest
Volume: 120
Pages: 883-893
Peer Reviewed
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