Identification and quantitation of low abundant tumor marker candidates in plasma derived from cancer tissue using recent proteomic technologies

2011 Fiscal Year Final Research Report

• Project/Area Number: 21390354
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: General surgery
• Research Institution: National Institute of Biomedical Innovation
• Principal Investigator: TOMONAGA Takeshi 独立行政法人医薬基盤研究所, プロテオームリサーチプロジェクト, プロジェクトリーダー (80227644)
• Co-Investigator(Kenkyū-buntansha): MATSUBARA Hisahiro 千葉大学, 大学院・医学研究院, 教授 (20282486) ; NOMURA Fumio 千葉大学, 大学院・医学研究院, 教授 (80164739) ; NISHIMURA Motoi 千葉大学, 大学院・医学研究院, 助教 (80400969)
• Project Period (FY): 2009 – 2011
• Keywords: プロテオーム / ペプチド / 癌 / 腫瘍マーカー / 診断

Research Abstract

Recent advance of proteomic technology have contributed to identify biomarkers for various disease, however, only a few proteins have been used in practical application. This study was aimed to identify and quantitate low abundant tumor marker candidates in plasma derived from cancer tissue using recent proteomic technologies. About 5000 membrane proteins from colorectal and breast cancer tissues were identified respectively. Among them, about 100 colorectal and 20 breast cancer biomarker candidate proteins were verified by SRM/MRM method. Some of the candidate proteins were detected in human serum.

Research Products

• [Journal Article] Plectin promotes migration and invasion of cancer cells and is a novel prognostic marker for head and neck squamous cell carcinoma (2012)
  • Author(s): Katada K, Tomonaga T, Satoh M, Matsushita K, Tonoike Y, Kodera Y, Hanazawa T, Nomura F, Okamoto Y
  • Journal Title: J Proteomics Volume: 75 Pages: 1803-15
  • URL: http://www.ncbi.nlm.nih.gov/pubmed/22245045
• [Journal Article] Proteomic study of malignant pleural mesothelioma by laser microdissection and two-dimensional difference gel electrophoresis identified cathepsin D as a novel candidate for a differential diagnosis biomarker (2012)
  • Author(s): Hosako M, Muto T, Nakamura Y, Tsuta K, Tochigi N, Tsuda H, Asamura H, Tomonaga T, Kawai A, Kondo T
  • Journal Title: J Proteomics Volume: 75 Pages: 833-44
  • URL: http://www.ncbi.nlm.nih.gov/pubmed/22051404
• [Journal Article] An alternative splicing isoform of eukaryotic initiation factor 4H promotes tumorigenesis in vivo and is a potential therapeutic target for human cancer (2011)
  • Author(s): Wu D, Matsushita K, Matsubara H, Nomura F, Tomonaga T
  • Journal Title: Int J Cancer Volume: 128 Pages: 1018-30
  • URL: http://www.ncbi.nlm.nih.gov/pubmed/20473909
• [Journal Article] Adhesion molecule periplakin is involved in cellular movement and attachment in pharyngeal squamous cancer cells (2011)
  • Author(s): Tonoike Y., Matsushita K., Tomonaga T., Katada K., Tanaka N., Shimada H., Nakatani, Y., Okamoto, Y., Nomura, F
  • Journal Title: BMC Cell Biol Volume: 12 Pages: 41
  • URL: http://www.ncbi.nlm.nih.gov/pubmed/21951621
• [Journal Article] The measurement of a fibrinogen alpha C-chain 5.9kDa fragment(FIC 5.9) using MALDI-TOF MS and a stable isotope-labeled peptide standard dilution (2011)
  • Author(s): Sogawa K, Kodera Y, Noda K, Ishizuka Y, Yamada M, Umemura H, Maruyama K, Tomonaga T, Yokosuka O, Nomura F
  • Journal Title: Clin Chim Acta Volume: 412 Pages: 1094-9
  • URL: http://www.ncbi.nlm.nih.gov/pubmed/21354123
• [Journal Article] Global expression study in colorectal cancer on proteins with alkaline isoelectric point by two-dimensional difference gel electrophoresis (2011)
  • Author(s): Muto T, Taniguchi H, Kushima R, Tsuda H, Yonemori H, Chen C, Sugihara Y, Sakamoto K, Kobori Y, Palmer H, Nakamura Y, Tomonaga T, Tanaka H, Mizushima H, Fujita S, Kondo T
  • Journal Title: J Proteomics Volume: 74 Pages: 858-73
  • URL: http://www.ncbi.nlm.nih.gov/pubmed/21385629
• [Journal Article] Synergistic effect of non-transmissible Sendai virus vector encoding the c-myc suppressor FUSE-binding protein-interacting repressor plus cisplatin in treatment of malignant pleural mesothelioma (2011)
  • Author(s): Kitamura A, Matsushita K, Takiguchi Y, Shimada H, Tada Y, Yamanaka M, Hiroshima K, Tagawa M, Tomonaga T, Matsubara H, Inoue M, Hasegawa M, Sato Y, Levens D, Tatsumi K, Nomura F
  • Journal Title: Cancer Sci Volume: 102 Pages: 1366-73
  • DOI: doi:10.1111/j.1349-7006.2011.01931.x.
• [Journal Article] Serum levels of complement C4 fragments correlate with disease activity in multiple sclerosis : proteomic analysis (2010)
  • Author(s): Sawai S, Umemura H, Mori M, Satoh M, Hayakawa S, Kodera Y, Tomonaga T, Kuwabara S, Nomura F
  • Journal Title: J Neuroimmunol Volume: 218 Pages: 112-5
  • URL: http://www.ncbi.nlm.nih.gov/pubmed/19923011
• [Journal Article] Reduction of novel circulating long-chain fatty acids in colorectal cancer patients is independent of tumor burden and correlates with age (2010)
  • Author(s): Ritchie SA, Heath D, Yamazaki Y, Grimmalt B, Kavianpour A, Krenitsky K, Elshoni H, Takemasa I, Miyake M, Sekimoto M, Monden M, Tomonaga T, Matsubara H, Sogawa K, Matsushita K, Nomura F, Goodenowe DB
  • Journal Title: BMC Gastroenterol Volume: 10 Pages: 140
  • URL: http://www.ncbi.nlm.nih.gov/pubmed/21114854
• [Journal Article] Phosphorylation statuses at different residues of lamin B2, B1, and A/C dynamically and independently change throughout the cell cycle (2010)
  • Author(s): Kuga T, Nozaki N, Matsushita K, Nomura F, Tomonaga T
  • Journal Title: Exp Cell Res Volume: 316 Pages: 2301-12
  • URL: http://www.ncbi.nlm.nih.gov/pubmed/20580708
• [Journal Article] High-yield peptide-extraction method for the discovery of subnanomolar biomarkers from small serum samples (2010)
  • Author(s): Kawashima Y, Fukutomi T, Tomonaga T, Takahashi h, Nomura F, Maeda T, Kodera Y
  • Journal Title: J Proteome Res Volume: 9 Pages: 1694-705
  • URL: http://www.ncbi.nlm.nih.gov/pubmed/20184378
• [Journal Article] The isolation and identification of apolipoprotein C-I in hormone-refractory prostate cancer using surface-enhanced laser desorption/ionization time-of-flight mass spectrometry (2010)
  • Author(s): Yamamoto-Ishikawa K, Suzuki H, Nezu M, Kamiya N, Imamoto T, Komiya A, Sogawa K, Tomonaga T, Nomura F, Ichikawa T
  • Journal Title: Asian J Androl Volume: 11 Pages: 299-307
  • URL: http://www.ncbi.nlm.nih.gov/pubmed/19182819
• [Journal Article] Effects of the time intervals between venipuncture and serum preparation for serum peptidome analysis by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (2009)
  • Author(s): Umemura H, Nezu M, Kodera Y, Satoh M, Kimura A, Tomonaga T, Nomura F
  • Journal Title: Clin Chim Acta Volume: 406 Pages: 179-80
  • URL: http://www.ncbi.nlm.nih.gov/pubmed/19520068
• [Journal Article] c-myc suppressor FBP-interacting repressor for cancer diagnosis and therapy (2009)
  • Author(s): Matsushita K, Tomonaga T, Kajiwara T, Shimada H, Itoga S, Hiwasa T, Kubo S, Ochiai T, Matsubara H, Nomura F
  • Journal Title: Front Biosci Volume: 14 Pages: 3401-8
  • URL: http://www.ncbi.nlm.nih.gov/pubmed/19273283
• [Journal Article] YKL-40 identified by proteomic analysis as a biomarker of sepsis (2009)
  • Author(s): Hattori N, Oda S, Sadahiro T, Nakamura M, Abe R, Shinozaki K, Nomura F, Tomonaga T, Matsushita K, Kodera Y, Sogawa K, Satoh M
  • Journal Title: Shock Volume: 32 Pages: 393-400
  • URL: http://www.ncbi.nlm.nih.gov/pubmed/19197227
• [Journal Article] Nm23-H1 is responsible for SUMO-2-involved DNA synthesis induction after X-ray irradiation in human cells (2009)
  • Author(s): Guo WZ, Sugaya S, Satoh M, Tomonaga T, Nomura F, Hiwasa T, Takiguchi M, Kita K, Suzuki N
  • Journal Title: Arch Biochem Biophys Volume: 486 Pages: 81-7
  • URL: http://www.ncbi.nlm.nih.gov/pubmed/19332021
• [Journal Article] Prohibitin in squamous cell carcinoma of the lung : Its expression and possible clinical significance
  • Author(s): Guo F, Hiroshima K, Wu D, Satoh M, Abulazi M, Yoshino I, Tomonaga T, Nomura F, Nakatani Y
  • Journal Title: Human pathology Volume: (in press)
  • URL: http://www.ncbi.nlm.nih.gov/pubmed/22304787
• [Journal Article] The Application of a Three-Step Proteome Analysis for Identification of New Biomarkers of Pancreatic Cancer
  • Author(s): Abulaizi M, Tomonaga T, Satoh M, Sogawa K, Matsushita K, Kodera Y, Obul J, Takano S, Yoshitomi H, Miyazaki M, Nomura F
  • Journal Title: Int J Proteomics Volume: (in press)
  • URL: http://www.ncbi.nlm.nih.gov/pubmed/22091389
• [Presentation] Absolute quantitation of plasma biomarker peptides for Alzheimer disease at pico-molar level using SRM coupled with stable isotope standards and capture by anti-peptide antibodies (2011)
  • Author(s): Shio Watanabe, Shinji Tagami, Seizo Sano, Kumiko Yoshizawa-Kumagaye, Masahiko Tsunemi, Masayasu Okochi and Takeshi Tomonaga
  • Organizer: HUPO 2011 10^<th> World Congress
  • Place of Presentation: Geneva, Switzerland
  • Year and Date: 20110904-07
• [Presentation] 定量プロテオミクス (2011)
  • Author(s): 朝長毅
  • Organizer: 日本プロテオーム学会2011年会
  • Place of Presentation: 新潟
  • Year and Date: 20110728-30
• [Presentation] 近年のプロテオーム解析技術の進歩と循環器病研究への応用 (2011)
  • Author(s): 朝長毅
  • Organizer: 第11回Cardiovascular Frontier Conference
  • Place of Presentation: 東京
  • Year and Date: 2011-11-19
• [Presentation] 大規模定量プロテオミクスによる疾患バイオマーカー探索 (2011)
  • Author(s): 朝長毅
  • Organizer: 第131回質量分析関西談話会
  • Place of Presentation: 大阪
  • Year and Date: 2011-11-12
• [Presentation] 大規模定量プロテオミクスを用いた疾患バイオマーカー探索とSRMを基盤とした実用化へのアプローチ (2011)
  • Author(s): 朝長毅
  • Organizer: 第9回北里疾患プロテオーム研究会
  • Place of Presentation: 東京
  • Year and Date: 2011-07-27
• [Presentation] 最近のプロテオミクスの進歩～ターゲットプロテオミクス:翻訳後修飾解析とSRMを用いた絶対定量～ (2010)
  • Author(s): 朝長毅
  • Organizer: 第33回日本分子生物学会年会第83回日本生化学会大会合同大会
  • Place of Presentation: 神戸
  • Year and Date: 20101207-10
• [Presentation] 近年のプロテオミクス技術の進歩とそのがん研究への応用 (2010)
  • Author(s): 朝長毅
  • Organizer: 第69回日本癌学会学術総会
  • Place of Presentation: 大阪
  • Year and Date: 20100922-24
• [Presentation] 国内のプロテオミクス研究拠点の動向 (2010)
  • Author(s): 朝長毅
  • Organizer: 日本ヒトプロテオーム機構第8回大会第6回日本臨床プロテオーム研究会連合会
  • Place of Presentation: 千葉
  • Year and Date: 20100726-27
• [Presentation] 最新プロテオーム解析技術を用いた疾患関連バイオマーカー探索研究 (2009)
  • Author(s): 朝長毅
  • Organizer: 第82回日本生化学会大会(口頭)
  • Place of Presentation: 神戸
  • Year and Date: 20091021-24
• [Presentation] プロテオームリサーチセンターにおける疾患関連バイオマーカー探索研究 (2009)
  • Author(s): 朝長毅
  • Organizer: 日本ヒトプロテオーム機構第7回大会(口頭)
  • Place of Presentation: 東京
  • Year and Date: 20090727-28
• [Presentation] Validation and functional analysis of a tumor marker candidate, eIF4H isoform 1, identified by 2DE (2009)
  • Author(s): Tomonaga T, Wu D, Nomura F.
  • Organizer: 3^<rd> EuPA Congress
  • Place of Presentation: Stockholm
  • Year and Date: 20090614-17
• [Presentation] プロテオーム解析の最新事情と基盤研プロテオームリサーチセンターの現状 (2009)
  • Author(s): 朝長毅
  • Organizer: 彩都バイオサイエンスセミナー(口頭)
  • Place of Presentation: 大阪
  • Year and Date: 2009-11-26
• [Presentation] 疾患関連バイオマーカー探索研究の現状と今後の方向性 (2009)
  • Author(s): 朝長毅
  • Organizer: 第7回北里疾患プロテオーム研究会(口頭)
  • Place of Presentation: 神奈川
  • Year and Date: 2009-07-26
• [Remarks]
  • URL: http://www.nibio.go.jp/proteome/index.html