• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to project page

2011 Fiscal Year Final Research Report

Identification and quantitation of low abundant tumor marker candidates in plasma derived from cancer tissue using recent proteomic technologies

Research Project

  • PDF
Project/Area Number 21390354
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field General surgery
Research InstitutionNational Institute of Biomedical Innovation

Principal Investigator

TOMONAGA Takeshi  独立行政法人医薬基盤研究所, プロテオームリサーチプロジェクト, プロジェクトリーダー (80227644)

Co-Investigator(Kenkyū-buntansha) MATSUBARA Hisahiro  千葉大学, 大学院・医学研究院, 教授 (20282486)
NOMURA Fumio  千葉大学, 大学院・医学研究院, 教授 (80164739)
NISHIMURA Motoi  千葉大学, 大学院・医学研究院, 助教 (80400969)
Project Period (FY) 2009 – 2011
Keywordsプロテオーム / ペプチド / 癌 / 腫瘍マーカー / 診断
Research Abstract

Recent advance of proteomic technology have contributed to identify biomarkers for various disease, however, only a few proteins have been used in practical application. This study was aimed to identify and quantitate low abundant tumor marker candidates in plasma derived from cancer tissue using recent proteomic technologies. About 5000 membrane proteins from colorectal and breast cancer tissues were identified respectively. Among them, about 100 colorectal and 20 breast cancer biomarker candidate proteins were verified by SRM/MRM method. Some of the candidate proteins were detected in human serum.

  • Research Products

    (32 results)

All 2012 2011 2010 2009 Other

All Journal Article (18 results) Presentation (13 results) Remarks (1 results)

  • [Journal Article] Plectin promotes migration and invasion of cancer cells and is a novel prognostic marker for head and neck squamous cell carcinoma2012

    • Author(s)
      Katada K, Tomonaga T, Satoh M, Matsushita K, Tonoike Y, Kodera Y, Hanazawa T, Nomura F, Okamoto Y
    • Journal Title

      J Proteomics

      Volume: 75 Pages: 1803-15

    • URL

      http://www.ncbi.nlm.nih.gov/pubmed/22245045

  • [Journal Article] Proteomic study of malignant pleural mesothelioma by laser microdissection and two-dimensional difference gel electrophoresis identified cathepsin D as a novel candidate for a differential diagnosis biomarker2012

    • Author(s)
      Hosako M, Muto T, Nakamura Y, Tsuta K, Tochigi N, Tsuda H, Asamura H, Tomonaga T, Kawai A, Kondo T
    • Journal Title

      J Proteomics

      Volume: 75 Pages: 833-44

    • URL

      http://www.ncbi.nlm.nih.gov/pubmed/22051404

  • [Journal Article] An alternative splicing isoform of eukaryotic initiation factor 4H promotes tumorigenesis in vivo and is a potential therapeutic target for human cancer2011

    • Author(s)
      Wu D, Matsushita K, Matsubara H, Nomura F, Tomonaga T
    • Journal Title

      Int J Cancer

      Volume: 128 Pages: 1018-30

    • URL

      http://www.ncbi.nlm.nih.gov/pubmed/20473909

  • [Journal Article] Adhesion molecule periplakin is involved in cellular movement and attachment in pharyngeal squamous cancer cells2011

    • Author(s)
      Tonoike Y., Matsushita K., Tomonaga T., Katada K., Tanaka N., Shimada H., Nakatani, Y., Okamoto, Y., Nomura, F
    • Journal Title

      BMC Cell Biol

      Volume: 12 Pages: 41

    • URL

      http://www.ncbi.nlm.nih.gov/pubmed/21951621

  • [Journal Article] The measurement of a fibrinogen alpha C-chain 5.9kDa fragment(FIC 5.9) using MALDI-TOF MS and a stable isotope-labeled peptide standard dilution2011

    • Author(s)
      Sogawa K, Kodera Y, Noda K, Ishizuka Y, Yamada M, Umemura H, Maruyama K, Tomonaga T, Yokosuka O, Nomura F
    • Journal Title

      Clin Chim Acta

      Volume: 412 Pages: 1094-9

    • URL

      http://www.ncbi.nlm.nih.gov/pubmed/21354123

  • [Journal Article] Global expression study in colorectal cancer on proteins with alkaline isoelectric point by two-dimensional difference gel electrophoresis2011

    • Author(s)
      Muto T, Taniguchi H, Kushima R, Tsuda H, Yonemori H, Chen C, Sugihara Y, Sakamoto K, Kobori Y, Palmer H, Nakamura Y, Tomonaga T, Tanaka H, Mizushima H, Fujita S, Kondo T
    • Journal Title

      J Proteomics

      Volume: 74 Pages: 858-73

    • URL

      http://www.ncbi.nlm.nih.gov/pubmed/21385629

  • [Journal Article] Synergistic effect of non-transmissible Sendai virus vector encoding the c-myc suppressor FUSE-binding protein-interacting repressor plus cisplatin in treatment of malignant pleural mesothelioma2011

    • Author(s)
      Kitamura A, Matsushita K, Takiguchi Y, Shimada H, Tada Y, Yamanaka M, Hiroshima K, Tagawa M, Tomonaga T, Matsubara H, Inoue M, Hasegawa M, Sato Y, Levens D, Tatsumi K, Nomura F
    • Journal Title

      Cancer Sci

      Volume: 102 Pages: 1366-73

    • DOI

      doi:10.1111/j.1349-7006.2011.01931.x.

  • [Journal Article] Serum levels of complement C4 fragments correlate with disease activity in multiple sclerosis : proteomic analysis2010

    • Author(s)
      Sawai S, Umemura H, Mori M, Satoh M, Hayakawa S, Kodera Y, Tomonaga T, Kuwabara S, Nomura F
    • Journal Title

      J Neuroimmunol

      Volume: 218 Pages: 112-5

    • URL

      http://www.ncbi.nlm.nih.gov/pubmed/19923011

  • [Journal Article] Reduction of novel circulating long-chain fatty acids in colorectal cancer patients is independent of tumor burden and correlates with age2010

    • Author(s)
      Ritchie SA, Heath D, Yamazaki Y, Grimmalt B, Kavianpour A, Krenitsky K, Elshoni H, Takemasa I, Miyake M, Sekimoto M, Monden M, Tomonaga T, Matsubara H, Sogawa K, Matsushita K, Nomura F, Goodenowe DB
    • Journal Title

      BMC Gastroenterol

      Volume: 10 Pages: 140

    • URL

      http://www.ncbi.nlm.nih.gov/pubmed/21114854

  • [Journal Article] Phosphorylation statuses at different residues of lamin B2, B1, and A/C dynamically and independently change throughout the cell cycle2010

    • Author(s)
      Kuga T, Nozaki N, Matsushita K, Nomura F, Tomonaga T
    • Journal Title

      Exp Cell Res

      Volume: 316 Pages: 2301-12

    • URL

      http://www.ncbi.nlm.nih.gov/pubmed/20580708

  • [Journal Article] High-yield peptide-extraction method for the discovery of subnanomolar biomarkers from small serum samples2010

    • Author(s)
      Kawashima Y, Fukutomi T, Tomonaga T, Takahashi h, Nomura F, Maeda T, Kodera Y
    • Journal Title

      J Proteome Res

      Volume: 9 Pages: 1694-705

    • URL

      http://www.ncbi.nlm.nih.gov/pubmed/20184378

  • [Journal Article] The isolation and identification of apolipoprotein C-I in hormone-refractory prostate cancer using surface-enhanced laser desorption/ionization time-of-flight mass spectrometry2010

    • Author(s)
      Yamamoto-Ishikawa K, Suzuki H, Nezu M, Kamiya N, Imamoto T, Komiya A, Sogawa K, Tomonaga T, Nomura F, Ichikawa T
    • Journal Title

      Asian J Androl

      Volume: 11 Pages: 299-307

    • URL

      http://www.ncbi.nlm.nih.gov/pubmed/19182819

  • [Journal Article] Effects of the time intervals between venipuncture and serum preparation for serum peptidome analysis by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry2009

    • Author(s)
      Umemura H, Nezu M, Kodera Y, Satoh M, Kimura A, Tomonaga T, Nomura F
    • Journal Title

      Clin Chim Acta

      Volume: 406 Pages: 179-80

    • URL

      http://www.ncbi.nlm.nih.gov/pubmed/19520068

  • [Journal Article] c-myc suppressor FBP-interacting repressor for cancer diagnosis and therapy2009

    • Author(s)
      Matsushita K, Tomonaga T, Kajiwara T, Shimada H, Itoga S, Hiwasa T, Kubo S, Ochiai T, Matsubara H, Nomura F
    • Journal Title

      Front Biosci

      Volume: 14 Pages: 3401-8

    • URL

      http://www.ncbi.nlm.nih.gov/pubmed/19273283

  • [Journal Article] YKL-40 identified by proteomic analysis as a biomarker of sepsis2009

    • Author(s)
      Hattori N, Oda S, Sadahiro T, Nakamura M, Abe R, Shinozaki K, Nomura F, Tomonaga T, Matsushita K, Kodera Y, Sogawa K, Satoh M
    • Journal Title

      Shock

      Volume: 32 Pages: 393-400

    • URL

      http://www.ncbi.nlm.nih.gov/pubmed/19197227

  • [Journal Article] Nm23-H1 is responsible for SUMO-2-involved DNA synthesis induction after X-ray irradiation in human cells2009

    • Author(s)
      Guo WZ, Sugaya S, Satoh M, Tomonaga T, Nomura F, Hiwasa T, Takiguchi M, Kita K, Suzuki N
    • Journal Title

      Arch Biochem Biophys

      Volume: 486 Pages: 81-7

    • URL

      http://www.ncbi.nlm.nih.gov/pubmed/19332021

  • [Journal Article] Prohibitin in squamous cell carcinoma of the lung : Its expression and possible clinical significance

    • Author(s)
      Guo F, Hiroshima K, Wu D, Satoh M, Abulazi M, Yoshino I, Tomonaga T, Nomura F, Nakatani Y
    • Journal Title

      Human pathology

      Volume: (in press)

    • URL

      http://www.ncbi.nlm.nih.gov/pubmed/22304787

  • [Journal Article] The Application of a Three-Step Proteome Analysis for Identification of New Biomarkers of Pancreatic Cancer

    • Author(s)
      Abulaizi M, Tomonaga T, Satoh M, Sogawa K, Matsushita K, Kodera Y, Obul J, Takano S, Yoshitomi H, Miyazaki M, Nomura F
    • Journal Title

      Int J Proteomics

      Volume: (in press)

    • URL

      http://www.ncbi.nlm.nih.gov/pubmed/22091389

  • [Presentation] Absolute quantitation of plasma biomarker peptides for Alzheimer disease at pico-molar level using SRM coupled with stable isotope standards and capture by anti-peptide antibodies2011

    • Author(s)
      Shio Watanabe, Shinji Tagami, Seizo Sano, Kumiko Yoshizawa-Kumagaye, Masahiko Tsunemi, Masayasu Okochi and Takeshi Tomonaga
    • Organizer
      HUPO 2011 10^<th> World Congress
    • Place of Presentation
      Geneva, Switzerland
    • Year and Date
      20110904-07
  • [Presentation] 定量プロテオミクス2011

    • Author(s)
      朝長毅
    • Organizer
      日本プロテオーム学会2011年会
    • Place of Presentation
      新潟
    • Year and Date
      20110728-30
  • [Presentation] 近年のプロテオーム解析技術の進歩と循環器病研究への応用2011

    • Author(s)
      朝長毅
    • Organizer
      第11回Cardiovascular Frontier Conference
    • Place of Presentation
      東京
    • Year and Date
      2011-11-19
  • [Presentation] 大規模定量プロテオミクスによる疾患バイオマーカー探索2011

    • Author(s)
      朝長毅
    • Organizer
      第131回質量分析関西談話会
    • Place of Presentation
      大阪
    • Year and Date
      2011-11-12
  • [Presentation] 大規模定量プロテオミクスを用いた疾患バイオマーカー探索とSRMを基盤とした実用化へのアプローチ2011

    • Author(s)
      朝長毅
    • Organizer
      第9回北里疾患プロテオーム研究会
    • Place of Presentation
      東京
    • Year and Date
      2011-07-27
  • [Presentation] 最近のプロテオミクスの進歩~ターゲットプロテオミクス:翻訳後修飾解析とSRMを用いた絶対定量~2010

    • Author(s)
      朝長毅
    • Organizer
      第33回日本分子生物学会年会第83回日本生化学会大会合同大会
    • Place of Presentation
      神戸
    • Year and Date
      20101207-10
  • [Presentation] 近年のプロテオミクス技術の進歩とそのがん研究への応用2010

    • Author(s)
      朝長毅
    • Organizer
      第69回日本癌学会学術総会
    • Place of Presentation
      大阪
    • Year and Date
      20100922-24
  • [Presentation] 国内のプロテオミクス研究拠点の動向2010

    • Author(s)
      朝長毅
    • Organizer
      日本ヒトプロテオーム機構第8回大会第6回日本臨床プロテオーム研究会連合会
    • Place of Presentation
      千葉
    • Year and Date
      20100726-27
  • [Presentation] 最新プロテオーム解析技術を用いた疾患関連バイオマーカー探索研究2009

    • Author(s)
      朝長毅
    • Organizer
      第82回日本生化学会大会(口頭)
    • Place of Presentation
      神戸
    • Year and Date
      20091021-24
  • [Presentation] プロテオームリサーチセンターにおける疾患関連バイオマーカー探索研究2009

    • Author(s)
      朝長毅
    • Organizer
      日本ヒトプロテオーム機構第7回大会(口頭)
    • Place of Presentation
      東京
    • Year and Date
      20090727-28
  • [Presentation] Validation and functional analysis of a tumor marker candidate, eIF4H isoform 1, identified by 2DE2009

    • Author(s)
      Tomonaga T, Wu D, Nomura F.
    • Organizer
      3^<rd> EuPA Congress
    • Place of Presentation
      Stockholm
    • Year and Date
      20090614-17
  • [Presentation] プロテオーム解析の最新事情と基盤研プロテオームリサーチセンターの現状2009

    • Author(s)
      朝長毅
    • Organizer
      彩都バイオサイエンスセミナー(口頭)
    • Place of Presentation
      大阪
    • Year and Date
      2009-11-26
  • [Presentation] 疾患関連バイオマーカー探索研究の現状と今後の方向性2009

    • Author(s)
      朝長毅
    • Organizer
      第7回北里疾患プロテオーム研究会(口頭)
    • Place of Presentation
      神奈川
    • Year and Date
      2009-07-26
  • [Remarks]

    • URL

      http://www.nibio.go.jp/proteome/index.html

URL: 

Published: 2013-07-31  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi