2011 Fiscal Year Final Research Report
Interacti on between mesenchymal and cancer stem cells in pancreatic cancer
| Project/Area Number |
21390371
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Digestive surgery
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| Research Institution | Chiba University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
MIYAZAKI Masaru 千葉大学, 大学院・医学研究院, 教授 (70166156)
KIMURA Fumio 千葉大学, 大学院・医学研究院, 准教授 (70334208)
TAKAHARA Yoshihiro 千葉大学, 医学部附属病院, 医員 (90533290)
TAKANO Shigetsugu 千葉大学, 医学部附属病院, 助教 (20436380)
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| Project Period (FY) |
2009 – 2011
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| Keywords | 膵臓癌 / 癌幹細胞 / 血管内皮細胞 / 間葉細胞 / 分子標的治療 |
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| Research Abstract |
We tried to elucidate the interaction between cancer mesenchymal cells and parenchymal cells, especially focused on the cancer stem cells. First we examined whether the FGF10 signal from mesenchymal cells might change the gene expression profile in cancer cells. However, we could not find out the adequate cancer cell culture condition with FGF10. Next we examined the expression pattern and the function of CD44, which is the cancer stem cells marker and cell adhesion molecule in pancreatic cancer. The strong expression of CD44 in cancer cells predicted the favorable prognosis of the patients with resected pancreatic cancer. Interestingly, the inhibition of CD44 expression in pancreatic cancer cell lines led to the increased cell migration ability of cells. Also this inhibition led to the formation of lamellipodia through modification of actin filament. We also found Cofilin induced the cell migration ability by comprehensive analysis of protein expression. These data suggest that the cell migration of pancreatic cells was controlled by complicated molecular mechanisms.
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