2011 Fiscal Year Final Research Report
Development of diagnostic system of early hepatocellular carcinoma by methylation of multiple genes
| Project/Area Number |
21390379
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Digestive surgery
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| Research Institution | Yamaguchi University |
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Principal Investigator |
OKA Masaaki 山口大学, 大学院・医学系研究科, 教授 (70144946)
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| Co-Investigator(Kenkyū-buntansha) |
IIZUKA Norio 山口大学, 医学部附属病院, 准教授 (80332807)
HAMAMOTO Yoshihiko 山口大学, 大学院・医学系研究科, 教授 (90198820)
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| Project Period (FY) |
2009 – 2011
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| Keywords | 肝癌 / メチル化遺伝子 / セルフリーDNA |
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| Research Abstract |
There are few blood tests for an efficient detection of hepatocellular carcinoma(HCC) associated with hepatitis C virus(HCV) infection. The abilities of quantitative analyses of 7 genes hypermethylation in serum DNA, -fetoprotein(AFP) and prothrombin-induced vitamin K absence II(PIVKA-II), and various combinations to detect HCC were evaluated. An optimal hybrid detector, built using data for 2 methylated genes(SPINT2 and SRD5A2), AFP, and PIVKA-II, achieved the most satisfactory ability to detect HCC in the training cohort(n=164) and an independent validation cohort(n=258). In the validation cohort, accuracy, sensitivity, and specificity of the hybrid detector for detection of HCC were 81. 4%, 73. 2%, and 87. 7%, respectively. Notably, even when detecting HCC 2cm in diameter, the hybrid detector maintained markedly high abilities(84. 6% accuracy, 72. 2% sensitivity, 87. 7% specificity). These results show that the optimal hybrid blood detector can detect HCV-related HCC more accurately.
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[Journal Article] Efficient detection of hepatocellular carcinoma by a hybrid blood test of epigenetic and classical protein markers2011
Author(s)
Iizuka N, Oka M, Sakaida I, Moribe T, Miura T, Kimura N, Tamatsukuri S, Ishitsuka H, Uchida K, Terai S, Yamashita S, Okita K, Sakata K, Karino Y, Toyota J, Ando E, Ide T, Sata M, Tsunedomi R, Tsutsui M, Iida M, Tokuhisa Y, Sakamoto K, Tamesa T, Fujita Y,
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Journal Title
Clin Chim Acta
Volume: Vol.412
Pages: 152-158
Peer Reviewed
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[Journal Article] Up-regulation of 42 kDa Tubulin Alpha-6 Chain Fragment in Well-differentiated Hepatocellular Carcinoma Tissues from Patients Infected with Hepatitis C Virus2011
Author(s)
Kuramitsu Y, Takashima M, Yokoyama Y, Iizuka N, Tamesa T, Akada JK, Wang Y, Toda T, Sakaida I, Okita K, Oka M, Nakamura K
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Journal Title
Anticancer Res. 2011 Anticancer Res
Volume: Vol 31(10)
Pages: 3331-3336
Peer Reviewed
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[Journal Article] Preoperative assessment of methylated cyclin D2 gene circulating in the blood for prediction of early intrahepatic recurrence of hepatocellular carcinoma after hepatectomy2010
Author(s)
Tsutsui M, Iizuka N, Moribe T, Miura T, Kimura N, Tamatsukuri S, Ishitsuka H, Fujita Y, Hamamoto Y, Tsunedomi R, Iida M, Tokuhisa Y, Sakamoto K, Tamesa T, Sakaida I, Oka M
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Journal Title
Clin Chim Acta
Volume: Vol.411
Pages: 516-520
Peer Reviewed
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[Journal Article] Methylation of multiple genes as molecular markers for diagnosis of a small, well-differentiated hepatocellular carcinoma2009
Author(s)
Moribe T, Iizuka N, Miura M, Kimura N, Tamatsukuri S, Ishitsuka H, Hamamoto Y, Sakamoto S, Tamesa T, Oka M
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Journal Title
Int J Cancer
Volume: Vol.125
Pages: 388-397
Peer Reviewed
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