2011 Fiscal Year Final Research Report
Research of bone and cartilage regulation by NF-kB signal for innovative targeted therapy.
| Project/Area Number |
21390415
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Orthopaedic surgery
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| Research Institution | The University of Tokyo |
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Principal Investigator |
ITO Hideya 東京大学, 医学部附属病院, 助教 (30436464)
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| Co-Investigator(Kenkyū-buntansha) |
ITO Hideya 東京大学, 医学部附属病院, 助教 (30436464)
OGATA Naoshi 東京大学, 医学部附属病院, 講師 (10361495)
CHIKUDA Hirotaka 東京大学, 医学部附属病院, 助教 (30345219)
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| Project Period (FY) |
2009 – 2011
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| Keywords | 骨・軟骨代謝学 / NF-kB |
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| Research Abstract |
Among transcription factors whose binding motifs were identified in the highly-conserved regions between human and mouse SOX9 promoters, a nuclear factor kappa B(NF-kB) member RelA strongly activated the promoter activity. RelA and SOX9 were co-localized in the limb cartilage. Deletion, mutagenesis, and tandem-repeat analyses identified the core region responsive to RelA at the NF-kB binding motif to be around-250 bp of the human SOX9 promoter, and this was confirmed to show specific binding to RelA. RelA induced the chondrogenic differentiation parameters in HeLa and ATDC5 cells. In conclusion, we have identified RelA as a transcriptional factor for SOX9 induction and chondrogenic differentiation via binding to an NF-kB binding motif in the SOX9 promoter.
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