Investigation on molecular biological mechanism of diurnal micturition rhythm

2011 Fiscal Year Final Research Report

• Project/Area Number: 21390439
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Urology
• Research Institution: Hyogo Medical University (2011); Kyoto University (2009-2010)
• Principal Investigator: KANEMATSU Akihiro 兵庫医科大学, 医学部, 准教授 (90437202)
• Co-Investigator(Kenkyū-buntansha): OGAWA Osamu 京都大学, 医学研究科, 教授 (90260611) ; YOSHIMURA Koji 京都大学, 医学研究科, 准教授 (40397542) ; OKUBO Kazutoshi 京都大学, 医学研究科, 講師 (20511877) ; INOUE Takahiro 関西医科大学, 医学部, 助教 (80511881) ; NISHIYAMA Hiroyuki 筑波大学, 人間健康科学研究科, 教授 (20324642)
• Co-Investigator(Renkei-kenkyūsha): OKAMURA Hitoshi 京都大学, 薬学研究科, 教授 (60158813) ; TABATA Yasuhiko 京都大学, 再生医科学研究所, 教授 (50211371)
• Project Period (FY): 2009 – 2011
• Keywords: 時計遺伝子 / コネキシン43 / ギャップジャンクション / Rev-erbalpha / Sp-1 / 日内排尿リズム

Research Abstract

We discovered circadian oscillation of Connexin43(Cx43), a molecule controlling functional bladder capacity, is regulated thorough interaction by Rev-erbα, a gene related with the circadian clock, and Sp-1, a molecule unrelated with the clock. As a result, circadian rhythm is generated in gap junction activity among bladder smooth muscle cells and contributes to micturition rhythm, with bladder capacity larger during sleep phase than during active phase. Thus, circadian clock is a regulator of micturition rhythm.

Research Products

• [Journal Article] Involvement of urinary bladder Connexin43 and the circadian clock in coordination of diurnal micturition rhythm (2012)
  • Author(s): Negoro H, Kanematsu A, Doi M, Suadicani SO, Matsuo M, Imamura M, Okinami T, Nishikawa N, Oura T, Matsui S, Seo K, Tainaka M, Urabe S, Kiyokage E, Todo T, Okamura H, Tabata Y, Ogawa O.
  • Journal Title: Nat Commun Volume: 3 Pages: 809
  • Peer Reviewed
• [Journal Article] Regulation of connexin 43 by basic fibroblast growth factor in the bladder : transcriptional and behavioral implications (2011)
  • Author(s): Negoro H, Kanematsu A, Imamura M, Kimura Y, Matsuoka R, Tanaka M, Tabata Y, Ogawa O.
  • Journal Title: J Urol Volume: 185(6) Pages: 2398-404
  • Peer Reviewed
• [Presentation] 離乳直後のマウス排尿発達のAutomated Voided Stain on Paper(aVSOP)法による解析 (2012)
  • Author(s): 兼松明弘、根来宏光、今村正明、小川修
  • Organizer: 第21回日本小児泌尿器科学会総会
  • Place of Presentation: 岡山
  • Year and Date: 20120704-06
• [Presentation] Automated voided stain on paper(aVSOP)法は、マウスの日内排尿リズムの記録を可能とする (2011)
  • Author(s): 根来宏光、兼松明弘、杉野善雄、沖波武、西川信之、吉村耕治、岡村均、田畑泰彦、小川修
  • Organizer: 第17回日本排尿機能学会
  • Place of Presentation: 甲府
  • Year and Date: 20110929-1001
• [Presentation] Developmental analysis of micturition in infant mouse immediately after weaning measured by a new recording system, automated voided stain of paper(aVSOP) method (2011)
  • Author(s): Negoro H, Kanematsu A, Sugino Y, Matsuo M, Kimura Y, Nishikawa N, Okinami T, Imamura M, Yoshimura K, Okamura H, Tabata Y, Ogawa O
  • Organizer: American Urological Association(AUA)
  • Place of Presentation: Washington DC, USA
  • Year and Date: 20110514-19
• [Presentation] 時計遺伝子Rev-erbαによるコネキシン43の転写制御は日内排尿リズムの形成因子である (2011)
  • Author(s): 根来宏光、兼松明弘、今村正明、沖波武、西川信之、吉村耕治、Silvia O Suadicani、大浦智紀、松尾雅弘、岡村均、田畑泰彦、小川修
  • Organizer: 日本泌尿器科学会総会
  • Place of Presentation: 名古屋
  • Year and Date: 2011-04-22
• [Remarks]
  • URL: http://www.kyoto-u.ac.jp/ja/news_data/h/h1/news6/2012/120502_1.htm
• [Remarks] 京都大学webページ
  • URL: http://www.kyoto-u.ac.jp/en/news_data/h/h1/news6/2012/120502_1.htm