Searching newly targets and applying their antibodies for the periodontal disease, furthermore development of IT drugs.

2012 Fiscal Year Final Research Report

• Project/Area Number: 21390497
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Functional basic dentistry
• Research Institution: Nihon University
• Principal Investigator: ABIKO Yoshimitsu 日本大学, 松戸歯学部, 教授 (70050086)
• Project Period (FY): 2009 – 2012
• Keywords: 歯周病 / P. gingivalis / TDC60株 / 治療標的分子 / ゲノムプロジェクト / データーベース / IT創薬 / 抗体

Research Abstract

Analyzing the genomic information for TDC60 is expected to provide new insights into the mechanisms underlying the onset and progression of periodontitis. For that reason, we searched for the specific virulence factor in the TDC60 strain using the complete genome sequences, and found higher levels of a dipeptidase PepD-2, a minor fimbire-1 (Mfa1), a collagenolysis-related peptidase Dap2 compared to the W83 strain using 2-DE and MALDI-TOF mass spectrometry. The crystal structure of PepD and HBP35 showed that the overall folding and then analyzed their function.

Research Products

• [Journal Article] Expression of caveolin-1 in the early phase of beta-TCP implanted in dog mandible. (2013)
  • Author(s): Chou CT, Bhawal UK, Watanabe N,Kuboyama N, Chang WJ, Lee SY, Abiko Y
  • Journal Title: J Biomed Mater Res B Appl Biomater
  • DOI: doi:10.1002/jbm.b.32884
  • Peer Reviewed
• [Journal Article] Silk fibroin-based scaffolds for bone regeneration (2013)
  • Author(s): Kuboyama N, Kiba H, Arai K, Uchida R, Tanimoto Y, Bhawal UK, Abiko Y, Miyamoto S, Knight D, Asakura T, Nishiyama N.
  • Journal Title: J Biomed Mater Res B Appl Biomater Volume: Vol.101 Pages: 295-302
  • DOI: doi:10.1002/jbm.b.32839
  • Peer Reviewed
• [Journal Article] Fibroblast growth factor 2 and forskolin induce mineralization-associated genes in two kinds of osteoblast-like cells (2012)
  • Author(s): Nakayama Y, Yang L, Takai H, Kaneko H
  • Journal Title: J Oral Sci Volume: Vol54 Pages: 251-9
  • Peer Reviewed
• [Journal Article] IL-1β-mediated up-regulation of DEC1 in human gingiva cells via the Akt (2012)
  • Author(s): Bhawal UK, Ito Y, Tanimoto K, Sato F, Fujimoto K, Kawamoto T, Sasahira T, Hamada N, Kuniyasu H, Arakawa H, Kato Y
  • Journal Title: J Cell Biochem Volume: Vol113 Pages: 3246-53
  • DOI: doi:10.1002/jcb.24205.
  • Peer Reviewed
• [Journal Article] Identification by DNA microarray of genes involved in Candida albicans-treated gingival epithelial (2012)
  • Author(s): Ikuta T, Bhawal UK, Tsushima K, Aoki A
  • Journal Title: J Oral Pathol Med. Volume: Vol41(10) Pages: 769-78
  • DOI: doi:10.1111/j.1600-0714.2012.01149.x.
  • Peer Reviewed
• [Journal Article] Sublingual vaccination with fusion protein consisting of the functional domain of hemagglutinin A of Porphyromonas gingivalis and Escherichia coli maltose-binding protein elicits protective immunity in the oralcavity. (2012)
  • Author(s): Yuzawa S, Kurita-Ochiai T, Hashizume T,Kobayashi R, Abiko Y, Yamamoto M.
  • Journal Title: FEMS Immunol Med Microbiol. Volume: Vol64 Pages: 265-72
  • DOI: doi:10.1111/j.1574-695X.2011.00895.x
  • Peer Reviewed
• [Journal Article] Transition metal ions induce carnosinase activity in PepD-homologous protein from Porphyromonas gingivalis. (2012)
  • Author(s): Aoki A, Shibata Y, Okano S, Maruyama F, Amano A, Nakagawa I, Abiko Y
  • Journal Title: Microb Pathog. Volume: Vol52 Pages: 17-24
  • DOI: doi:10.1016/j.micpath.2011.09.003.
  • Peer Reviewed
• [Journal Article] Characterization of human-type monoclonal antibod es against reduced form of hemin binding protein 35 from Porphyromonas gingivalis. (2011)
  • Author(s): Shibata Y, Okano S, Shiroza T, Tahara T, Nakazawa K, Kataoka S, Ishida I, Kobayashi T, Yoshie H, Abiko Y
  • Journal Title: J Periodontal Res. Volume: Vol46 Pages: 673-81
  • DOI: doi:10.1111/j.1600-0765.2011.01389.x.
  • Peer Reviewed
• [Journal Article] Low-level laser irradiation treatment reduces CCL2 expression in rat rheumatoid synovia via a chemokine signaling pathway. (2011)
  • Author(s): Zhang L, Zhao J, Kuboyama N, Abiko Y
  • Journal Title: Lasers Med Sci. Volume: Vol26 Pages: 707-17
  • DOI: doi:10.1007/s10103-011-0917-y.
  • Peer Reviewed
• [Journal Article] Acute application of cisplatin affects methylationstatus in neuroblastoma cells. (2011)
  • Author(s): Tabata K, Sakai H, Nakajima R, Saya-Nishimura R, Motani K, Okano S, Shibata Y, Abiko Y, Suzuki T
  • Journal Title: Oncol Rep. Volume: Vol25 Pages: 1655-60.
  • DOI: doi:10.3892/or.2011.1222.
  • Peer Reviewed
• [Journal Article] Connexin 43 expression at an early stage in dog mandibles by β-TCP. (2011)
  • Author(s): Hayashi M, Takahashi T, Kawaguchi K,Watanabe T, Zhao J, Abiko Y
  • Journal Title: Dent Mater J. Volume: Vol30(1) Pages: 58-65
  • Peer Reviewed
• [Journal Article] Transcriptome analysis of β-TCP (2011)
  • Author(s): Zhao J, Watanabe T, Bhawal UK, Kubota E, Abiko Y
  • Journal Title: Bone. Volume: Vol48 Pages: 864-77
  • DOI: doi:10.1016/j.bone.2010.11.019.
  • Peer Reviewed
• [Journal Article] immunization with a fusion protein consisting of the hemagglutinin A antigenic region and the maltose-binding protein elicits CD11c(+) CD8(+) dendritic cells for induced long-term (2011)
  • Author(s): Du Y, Hashizume T, Kurita-Ochiai T, Yuzawa S, Abiko Y, Yamamoto M. Nasal
  • Journal Title: Infect Immun. Volume: Vol79(2) Pages: 895-904
  • DOI: doi:10.1128/IAI.01203-10.
  • Peer Reviewed
• [Journal Article] Periodontal pathogen Aggregatibacter actinomycetemcomitans LPS induces mitochondria-dependent-apoptosis in human placental trophoblasts. (2011)
  • Author(s): Li Y, Shibata Y, Zhang L, Kuboyama N, Abiko Y
  • Journal Title: Placenta. Volume: Vol32(1) Pages: 11-9
  • DOI: doi:10.1016/j.placenta.2010.10.007.
  • Peer Reviewed
• [Journal Article] Oxidative stress induces phosphorylation of the ABC transporter, ATP-binding protein, in Porphyromonas gingivalis. (2010)
  • Author(s): Toyoda T, Okano S, Shibata Y, Abiko Y
  • Journal Title: J Oral Sci. Volume: Vol52(4) Pages: 561-6
  • Peer Reviewed
• [Journal Article] Effect of endothelial cell proliferation on atherogenesis: a role of p21(Sdi/Cip/Waf1) in monocyte adhesion to endothelial cells. (2010)
  • Author(s): Obikane H, Abiko Y, Ueno H, Kusumi Y, Esumi M, Mitsumata M.
  • Journal Title: Atherosclerosis. Volume: Vol212(1) Pages: 116-22
  • DOI: doi:10.1016/j.atherosclerosis.2010.05.029
  • Peer Reviewed
• [Journal Article] A monoclonal antibody against fimA type II Porphyromonas gingivalis inhibits IL-8 production in human gingival fibroblasts. (2010)
  • Author(s): Hijiya T, Shibata Y, Hayakawa M, Abiko Y
  • Journal Title: Hybridoma Volume: Vol29(3) Pages: 201-4
  • DOI: doi:10.1089/hyb.2009.0109.
  • Peer Reviewed
• [Journal Article] Hemin-binding protein 35 (HBP35) plays an important role in bacteria-mammalian cells interactions in Porphyromonas gingivalis. (2010)
  • Author(s): Hiratsuka K, Kiyama-Kishikawa M, Abiko Y
  • Journal Title: Microb Pathog. Volume: Vol48 Pages: 116-23
  • DOI: doi:10.1016/j.micpath.2010.01.001..
  • Peer Reviewed
• [Journal Article] Monoclonal antibodies produced against lipopolysaccharide from fimA Type II Porphyromonas gingivalis. (2009)
  • Author(s): Maruyama M, Hayakawa M, Zhang L, ShibataY, Abiko
  • Journal Title: Hybridoma Volume: Vol28(6) Pages: 431-4
  • DOI: doi:10.1089/hyb.2009.0055.
  • Peer Reviewed
• [Journal Article] IL-1β stimulates IL-8 production, including prostaglandin Ereceptor EP4-triggered pathways, in synoviocyte MH7A cells. (2009)
  • Author(s): Shibata Y, Kasai H, Shimada M, Koitabashi T, Arai T, Sai R, Terao H, Horikiri M, Negishi H, Miyazawa K, Abiko Y
  • Journal Title: Mol Med Rep. Volume: Vol2 Pages: 359-63
  • DOI: doi:10.3892/mmr_00000108.
  • Peer Reviewed
• [Presentation] Abiko, Gene Expression Profiles of Increased Expression of beta-defensin-2 in Keratinocyte (2013)
  • Author(s): M. Yamazaki, M. Saitoh, M. Nishimura, J. Sato, H. Saotoh, R. Takai, U.K. Bhawal, Y.
  • Organizer: IADR
  • Place of Presentation: Seattle (USA)
  • Year and Date: 20130320-0323
• [Presentation] Effect of blue LED on Porphyromonas gingivalis growth in vitro. (2013)
  • Author(s): C. Chui, K. Horatsuka, A. Aoki, Y. Takeuchi,Y. Abiko, Y. Izumi
  • Organizer: IADR
  • Place of Presentation: Seattle(USA)
  • Year and Date: 20130320-0323
• [Presentation] A study of 16S rRNA for bacterial gene expression analysis (2013)
  • Author(s): K. Hiratsuka, Y. Abiko
  • Organizer: IADR
  • Place of Presentation: Seattle(USA)
  • Year and Date: 20130320-0323
• [Presentation] Transcription factor DEC1 and Epithelial-Mesenchymal Transition (2013)
  • Author(s): U. Bahwal, Y. Abiko
  • Organizer: IADR
  • Place of Presentation: Seattle (USA)
  • Year and Date: 20130320-0323
• [Presentation] 新規歯周病治療標的分子PepDの結晶構造解析 (2013)
  • Author(s): 鈴木守・柴田恭子・藤原芳江・安孫子宜光
  • Organizer: 構研サイエンスフェスタつくば国際会議場エポカル
  • Place of Presentation: つくば国際会議場(茨城)
  • Year and Date: 20130314-0315
• [Presentation] 新規歯周病治療標的分子PepDの結晶構造解析 (2013)
  • Author(s): 鈴木守・柴田恭子・藤原芳江・安孫子宜光
  • Organizer: 第26回日本放射光学会年会放射光科学合同シンポジウ
  • Place of Presentation: 名古屋大学(愛知)
  • Year and Date: 20130112-0114
• [Presentation] Identification of a componentof LPS biosunthesis n Porphyromonas gingivalis (2012)
  • Author(s): M. Shoji, H. Ukitake, K. sato, Y. Shibata, M.Naito, J. Aduse-Opoku, Y. Abiko, M.A. Curtis, K. Nakayama
  • Organizer: 第60回国際歯科研究学会日本部会 総会・学術大会
  • Place of Presentation: 新潟コンベンションセンター(新潟)
  • Year and Date: 20121214-1215
• [Presentation] 新規歯周病標的分子PepDのX線結晶構造解析 (2012)
  • Author(s): 鈴木守、柴田恭子、藤原芳江、安孫子宜光
  • Organizer: 平成24年度日本結晶学会年会および総会
  • Place of Presentation: 東北大学片平キャンパス(宮城)
  • Year and Date: 20121025-1026
• [Presentation] FimA II型 P. gingivalis (TDC60)新規治療標的分子の探索-新規標的分子PepDの構造および機能解析- (2012)
  • Author(s): 柴田恭子、鈴木守、安孫子宜光
  • Organizer: 第54回歯科基礎医学会学術大会ならびに総会
  • Place of Presentation: 奥羽大学(福島)
  • Year and Date: 20120914-0915
• [Presentation] Development of the moleculartargeted therapy for eriodontal disease (2011)
  • Author(s): Y. Abiko
  • Organizer: Int Assoc Dent Res, Korean Division Annual Meetin
  • Place of Presentation: ソウル(韓国)
  • Year and Date: 2011-11-23
• [Presentation] IL-8 signaling pathway in Candida albicans-infected gingival epithelial cells. (2011)
  • Author(s): U. Bhawal, K. Tsushima, T. Ikuta, Y. Abiko
  • Organizer: 59^<th> Annual Meeting of Japanese Association for Dental Research
  • Place of Presentation: International Conference Center Hiroshima (Hiroshima)
  • Year and Date: 2011-10-08
• [Presentation] From diagnosis to periodontal therapy utilizing functional genomics (2011)
  • Author(s): Y. Abiko
  • Organizer: 59^<th>Annual Meeting of Japanese Association for Dental Research
  • Place of Presentation: International Conference Center Hiroshima (Hiroshima)
  • Year and Date: 2011-10-08
• [Presentation] ヒト口腔扁平上皮癌におけるPERIOD1(PER1)とPER3の発現 (2011)
  • Author(s): Bhawal Ujjal、伊藤由美、安孫子宜光
  • Organizer: 第53回歯科基礎医学会学術大会
  • Place of Presentation: 長良川国際会議場(岐阜)
  • Year and Date: 2011-10-01
• [Presentation] 炎症におけるDEC1とDEC2の役割 (2011)
  • Author(s): Bhawal Ujjal、伊藤由美、安孫子宜光
  • Organizer: 第53回歯科基礎医学会学術大会
  • Place of Presentation: 長良川国際会議場(岐阜)
  • Year and Date: 2011-10-01
• [Presentation] Expression of Caveolin in beta-TCP implanted in dg mandible. (2011)
  • Author(s): Ujjal K. Bhawal, Hu-Huang Chao Shotaro Kojima, Noboru Kuboyama, YoshimitsuAbiko
  • Organizer: 第11回日本大学口腔科学会学術大会
  • Place of Presentation: 日本大学松戸歯学部(千葉)
  • Year and Date: 2011-09-04
• [Presentation] Mitochondrial oxidativestress and less mineralization by hydrogenperoxide treatment in MC3T3-E1 cell line. (2011)
  • Author(s): Y Shibata, M Arai, S Okano, K Pugdee, TSato, Y Otsuka-Tanaka, J Mega, H J Majima, Y Ogata, Y Abiko
  • Organizer: 5th Biennial Meeting of Society for Free Radical Research-Asis (SFRR-Asia) andConference of Asian Society forMitochondrial Research and Medicine (ASMRM)
  • Place of Presentation: 鹿児島市民ホール(鹿児島)
  • Year and Date: 2011-09-02
• [Presentation] Molecular Mechanism of s-TCP in Accelerating Bone Formation and Wound Healing (2011)
  • Author(s): Y. Abiko
  • Organizer: The Annual Scientific Meeting of the Assoc Dental SocRepublic of China ADS-ROC
  • Place of Presentation: Taipei(Taiwan)
  • Year and Date: 2011-08-26
• [Presentation] IL-22がヒト歯根膜由来細胞の石灰化ノジュール形成及ぼす影響 (2011)
  • Author(s): 大山秀樹、小越菜保子、川辺陸記、安川陽子、安孫子宜光
  • Organizer: 日本歯周病学会2011春期学術大会(第54回)
  • Place of Presentation: 福岡国際会議場(福岡)
  • Year and Date: 2011-05-28
• [Presentation] ココアフラボノールの抗炎症作用による動脈硬化抑制作用の検討 (2011)
  • Author(s): 永安慎太郎、山下明子、鈴木茂樹、半井英雄、岩下未咲、熊本園子、安孫子宜光、西村英紀
  • Organizer: 日本歯周病学会2011春期学術大会(第54回)
  • Place of Presentation: 福岡国際会議場(福岡)
  • Year and Date: 2011-05-27
• [Presentation] IL-22存在下においてヒト歯根膜由来細胞が発現する伝子の網羅的解析 (2011)
  • Author(s): 川辺陸記、大山秀樹、安川陽子、小越奈保子、安孫子宜光
  • Organizer: 日本歯周病学会2011春期学術大会(第54回)
  • Place of Presentation: 福岡国際会議場(福岡)
  • Year and Date: 2011-05-27
• [Presentation] Reduction of ICAM-1 in Rat Rheumatoid Synovium by Laser Irradiation (2011)
  • Author(s): L. Zhang, N. Kuboyama, Y. Abiko
  • Organizer: IADR General Session
  • Place of Presentation: San Diego, Calif. (USA)
  • Year and Date: 2011-03-19
• [Presentation] Signal pathway analysis of the effectiveness of low-level laser irradiation in rheumatoid arthritis (2011)
  • Author(s): Abiko Yoshimitsu
  • Organizer: SPIE Photonics West 2010
  • Place of Presentation: San Francisco, Calif. (USA)
  • Year and Date: 2011-01-26
• [Presentation] 骨芽細胞への活性酸素刺激、歯周病原菌への酸素ストレスの機能ゲノム科学的考察 (2010)
  • Author(s): 安孫子宜光
  • Organizer: 第25回日本酸化ストレス学会関東支部会
  • Place of Presentation: タワーホール船堀(東京)
  • Year and Date: 2010-12-11
• [Presentation] Diagnosis to molecular target therapy for oral infectious diseases by bioinformatics technology (2010)
  • Author(s): Abiko Yoshimitsu
  • Organizer: The 1^<st>Global Congress of Chinese Dentists
  • Place of Presentation: 厦門(中国)
  • Year and Date: 2010-12-06
• [Presentation] 新興感染症・再興感染症への地球規模の取り組み『歯周病原細菌ゲノム解析による新規治療戦略』 (2010)
  • Author(s): 柴田恭子、安孫子宜光
  • Organizer: 平成22年度日本大学学部連携研究シンポジウム
  • Place of Presentation: 日本大学会館(東京)
  • Year and Date: 2010-12-03
• [Presentation] Functional genomic Study onthe mechanism of anti-inflammatory effect by low level laser irradiation (2010)
  • Author(s): 安孫子宜光
  • Organizer: 台湾歯科医学総会
  • Place of Presentation: 台北市(台湾)
  • Year and Date: 2010-11-29
• [Presentation] EnhancedHSPs Gene Expression in Aggregatibacter actinomycetemcomitans LPS-challenged Human Trophoblasts (2010)
  • Author(s): Y. LI, Y. SHIBATA, Y. ABIKO
  • Organizer: 第58回国際歯科研究学会日本部会(JADR)総会・学術大会
  • Place of Presentation: 九州歯科大学(福岡)
  • Year and Date: 2010-11-20
• [Presentation] Functional Genomic Study on Anti-inflammation by Low Level Laser Irradiation (2010)
  • Author(s): 安孫子宜光
  • Organizer: The 13^<th> Congress of Asian Pacific Association for Laser Medicine and Surgery
  • Place of Presentation: 信濃医療福祉センター(静岡)
  • Year and Date: 2010-10-08
• [Presentation] fimAII型P.gingivalis(TDC60)新規治療標的分子の探索- PepD-2の機能について (2010)
  • Author(s): 青木暁宣、柴田恭子、岡野総一郎、中川一路、丸山史人、天野敦雄、安孫子宜光
  • Organizer: 第52回歯科基礎医学会学術大会・総会
  • Place of Presentation: タワーホール船堀(東京)
  • Year and Date: 2010-09-22
• [Presentation] 高出力青色LEDの歯周病原菌に対する殺菌効果 (2010)
  • Author(s): チューチャントゥーン、青木章、竹内康雄、佐々木好幸、安孫子宜光、和泉雄一
  • Organizer: 第53回秋季日本歯周病学会学術大会
  • Place of Presentation: サンポートホール高松(香川)
  • Year and Date: 2010-09-19
• [Presentation] Transcriptome Analysis of s-TCP Implanted Dog Mandible (2010)
  • Author(s): J. Zhao, N. Kuboyama, T. Watanabe, Y. Abiko
  • Organizer: IADR General Session
  • Place of Presentation: Barcelona (Spain)
  • Year and Date: 2010-07-15
• [Presentation] Induction of Low Birth Weight by Periodontitis in Placenta Through Break-down of Balance between Apoptosis and Proliferation (2010)
  • Author(s): Y Li, Y Shibata, Y Abiko
  • Organizer: American Society for Microbiology (ASM) 110th
  • Place of Presentation: San Diego, CA (USA)
  • Year and Date: 2010-05-26
• [Presentation] Monoclonal antibodies neutralizing fimA type II Prophyromonas gingivalis virulent factors (2010)
  • Author(s): L Zhang, Y Shibata, Y Abiko
  • Organizer: American Society for Microbiology (ASM) 110th General Meeting
  • Place of Presentation: San Diego, CA (USA)
  • Year and Date: 2010-05-24
• [Presentation] レーザによる炎症性シグナ ル伝達系の抑制効果 (2009)
  • Author(s): 安孫子宜光
  • Organizer: 日本レーザー医学会
  • Place of Presentation: ホテルグランドヒル市ヶ谷(東京)
  • Year and Date: 2009-12-03
• [Presentation] P.gingivalisにおけるATPtransporterのリン酸化とLP産生への酸素刺激の影響 (2009)
  • Author(s): 豊田隆雄,岡野総一郎,柴田恭子,安孫子宜光
  • Organizer: 第52回秋季日本歯周病学会学術大会
  • Place of Presentation: 宮崎観光ホテル(宮崎)
  • Year and Date: 2009-10-11
• [Presentation] II型P.gingivalis(TDC60)に特異発現している70 kDaタンパク質分子について (2009)
  • Author(s): 青木暁宣、柴田恭子、岡野総一郎、中川一路、天野敦夫、安孫子宜光
  • Organizer: 第51回歯科基礎医学会学術大会・総会
  • Place of Presentation: 朱鷺メッセ(新潟)
  • Year and Date: 2009-09-11
• [Presentation] P.gingivalis赤血球凝集活性抑制抗体のインフルエンザに対する交叉反応性 (2009)
  • Author(s): 柴田恭子、岡野総一郎、細木弓子、安孫子宜光
  • Organizer: 口腔科学会
  • Place of Presentation: 日本大学松戸歯学部(千葉)
  • Year and Date: 2009-09-06
• [Presentation] Advancing entistry at theCrossroads of the World (2009)
  • Author(s): Yoshimitsu Abiko
  • Organizer: FDI Annual World Dental Congress
  • Place of Presentation: Singapore
  • Year and Date: 2009-09-05
• [Presentation] ヒト血清を用いた脂肪組織由来間葉系幹細胞の硬組織分化能の解析 (2009)
  • Author(s): 岩山智明、橋川智子、島袋善夫、菱川祥郎、小笹匡雄、柴田恭子、安孫子宜光、村上伸也
  • Organizer: 日本歯周病学会2009年春季学術大会(第52回)
  • Place of Presentation: 岡山コンベンションセンター(ママカリフォーラム)(岡山)
  • Year and Date: 2009-05-16
• [Presentation] マクロファージと共存する脂肪細胞はLPS刺激によってtoll-likereceptorを介するシグナルを増強する分子群を産生する (2009)
  • Author(s): 熊本園子、山下明子、曽我賢彦、岩本義博、岩下未咲、安孫子宜光、西村英紀
  • Organizer: 日本歯周病学会2009春季学術大会
  • Place of Presentation: 岡山コンベンションセンター(ママカリフォーラム)(岡山)
  • Year and Date: 2009-05-15
• [Book] 光アライアンス (2012)
  • Author(s): 柴田恭子,安孫子宜光
  • Total Pages: 8-12
• [Book] ビジュアル歯周病を科学する (2012)
  • Author(s): 安孫子宜光、平塚浩一、柴田恭子
  • Total Pages: 220-222
  • Publisher: クインテッセンス出版株式会社
• [Book] (2011)
  • Author(s): Yasuko Shibata, Yoshimitsu Abiko
  • Total Pages: 153-170
  • Publisher: INTECH Open Access Publisher