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2011 Fiscal Year Final Research Report

FEN1-related senescence

Research Project

  • PDF
Project/Area Number 21390504
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Pathobiological dentistry/Dental radiology
Research InstitutionNagasaki University

Principal Investigator

NAKAMURA Takashi  長崎大学, 大学院・医歯薬学総合研究科, 教授 (30172406)

Co-Investigator(Kenkyū-buntansha) SUMI Misa  長崎大学, 大学院・医歯薬学総合研究科, 准教授 (90284702)
HOTOKEZAKA Yuka  長崎大学, 大学病院, 講師 (10244089)
KIMURA Yasuo  長崎大学, 大学院・医歯薬学総合研究科, 助教 (30253686)
TASHIRO Shigeki  長崎大学, 大学院・医歯薬学総合研究科, 助教 (20300882)
SUMI Tadateru  長崎大学, 大学院・医歯薬学総合研究科, 助教 (80284701)
TAKAGI Yukinori  長崎大学, 大学院・医歯薬学総合研究科, 助教 (30295084)
EIDA Sato  長崎大学, 大学院・医歯薬学総合研究科, 助教 (80325662)
KATAYAMA Ikuo  長崎大学, 大学病院, 助教 (80295089)
SASAKI Miho  長崎大学, 大学病院, 助教 (10437874)
ICHIKAWA Youko  長崎大学, 大学病院, 助教 (90380857)
Project Period (FY) 2009 – 2011
KeywordsFEN1 / マウスモデル / 紫外線 / 腫瘍
Research Abstract

Here we investigated the effects of FEN1 ablation on growth of different cell types with or without intact p53 by using RNA interference. We found that the FEN1 ablation triggers senescence in p53-mutated T24 cells. However, transfection of the wild-type p53 into these T24 cells inhibited the senescence. On the other hand, HT116 cells with the wild-type p53 were not introduced to senescence by interfering FEN1 expression ; instead, the cells underwent apoptosis. Contrary in HT116 cells defective of p53 did not exhibit senescence or apoptosis after FEN1 siRNA treatment. Interestingly, ionizing irradiation(5Gy) caused senescence in p53-dfective HT116 cells that were treated with FEN1 siRNA. These results suggest that FEN1-induced senescence is p53-dependent and imply that this mechanism may be involved in UV-induced tumorigenesis.

  • Research Products

    (6 results)

All 2011 2010 2009

All Journal Article (3 results) (of which Peer Reviewed: 3 results) Presentation (3 results)

  • [Journal Article] Multiparametric MR imaging of sinonasal diseases : time-signal intensity curve-and apparent diffusion coefficient-based differentiation between benign and malignant lesions2011

    • Author(s)
      佐々木美穂、角美佐、榮田智、市川陽子、角忠輝、山田敏朗、中村卓
    • Journal Title

      AJNR Am J Neuroradiol

      Volume: 32(11) Pages: 2154-2159

    • Peer Reviewed
  • [Journal Article] Multiparametric MR imaging for the differentiation between benign And malignant salivary gland tumors2010

    • Author(s)
      榮田智、角美佐、中村卓
    • Journal Title

      J Magn Reson Imaging

      Volume: 31(3) Pages: 673-679

    • Peer Reviewed
  • [Journal Article] αNAC depletion as an initiation of ER stress-induced apoptosis in Hypoxia2009

    • Author(s)
      佛坂由可、van leyen K、Lo EH、Beatrix B、片山郁夫、Jin G, 中村卓
    • Journal Title

      Cell Death Differ

      Volume: 16(11) Pages: 1505-1514

    • Peer Reviewed
  • [Presentation] 細胞ストレス応答におけるg-taxilinの関与日本歯科放射線学会2011

    • Author(s)
      佛坂由可, 片山郁夫, 中村卓
    • Organizer
      第31回関西・九州合同地方会
    • Place of Presentation
      名古屋
    • Year and Date
      2011-12-10
  • [Presentation] In vitro measurement of diffusion coefficient2011

    • Author(s)
      榮田智, 佛坂由可, 片山郁夫, 山田敏朗, 中村卓
    • Organizer
      第52回日本歯科放射線学会総会・学術大会第18回国際歯顎顔面放射線学会併催
    • Place of Presentation
      広島
    • Year and Date
      2011-05-25
  • [Presentation] FEN1活性抑制による細胞老化について2010

    • Author(s)
      片山郁夫, 佛坂由可, 中村卓
    • Organizer
      第51回日本歯科放射線学会総会・学術大会
    • Place of Presentation
      横浜
    • Year and Date
      2010-04-24

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Published: 2013-07-31   Modified: 2016-01-14  

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