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2012 Fiscal Year Final Research Report

Survey on members of sucrose-world in Asian tropical reef, namely in Thailand

Research Project

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Project/Area Number 21405020
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section海外学術
Research Field Applied biochemistry
Research InstitutionHokkaido University

Principal Investigator

KIMURA Atsuo  北海道大学, 大学院・農学研究院, 教授 (90186312)

Co-Investigator(Kenkyū-buntansha) MORI Haruhide  北海道大学, 大学院・農学研究院, 准教授 (80241363)
Project Period (FY) 2009 – 2012
Keywordsショ糖 / 分泌 / 生物間相互作用
Research Abstract

Plants synthesize the starch in the leaves by photosynthesis. Leaf-starch is converted to sucrose, and transported to storage organ (e.g., tubers and fruit-bodies), followed by re-formation of starch (storage-starch). From many sites of bodies, plants secret sucrose, by which plants perform the cross-talk with environmental organisms (e.g., microorganisms and insects). Recently, it was found that huge amount of sucrose was secreted (this system is call as sucrose-world). The purpose of this research is survey of sucrose-world-members (environmental organisms and their enzymes) in Thailand, where high sunshine is available. As a result, the system of "sucrose -> polysaccharide -> secondary or ternary sugar" was investigated at sucrose secreted from roots. About flower bee, the enzyme converting sucrose to oligosaccharide was also investigated.

  • Research Products

    (28 results)

All 2013 2012 2011 2010 2009 Other

All Journal Article (17 results) (of which Peer Reviewed: 17 results) Presentation (10 results) Remarks (1 results)

  • [Journal Article] Enzymatic synthesis of acarviosyl-maltooligosaccharides using disproportionating enzyme 12013

    • Author(s)
      Tagami T, Tanaka Y, Okuyama M, Mori H, Kimura A
    • Journal Title

      Biosci Biotechnol Biochem

      Volume: 77(2) Pages: 312-319

    • DOI

      doi.org/10.1271/bbb.120473

    • Peer Reviewed
  • [Journal Article] Expression of a secretory α-glucosidase II from Apis cerana indica in Pichia pastoris and its characterization2013

    • Author(s)
      Kaewmuangmoon J, Kilaso M, Leartsakulpa-nich U, Kimura K, Kimura A, Chanchao C
    • Journal Title

      BMC Biotechnol

      Volume: 13 Pages: 16

    • DOI

      doi:10.1186/1472-6750-13-16.

    • Peer Reviewed
  • [Journal Article] A novel mechanism for promotion of quercetin glycoside absorption by megalo α-1,6-glucosaccharide in the rat small intestine2013

    • Author(s)
      Shinoki A, Lang W, Thawornkuno C, Kang HK, Kumagai Y, Okuyama M, Mori H, Kimura A, Ishizuka S, Hara H
    • Journal Title

      Food Chem

      Volume: 136(2) Pages: 293-296

    • DOI

      doi.org/10.1016/j.foodchem.2012.08.028

    • Peer Reviewed
  • [Journal Article] Characterization of some enzymatic properties of recombinant α-glucosidase III from the Thai honeybee, Apis cerana indica Fabricus2012

    • Author(s)
      Kaewmuangmoon J, Yoshiyama M, Kimura K, Okuyama M, Mori H, Kimura A, Chanchao C
    • Journal Title

      Afr J Biotechnol

      Volume: 11(96) Pages: 16220-16232

    • DOI

      doi:10.5897/AJB12.2402

    • Peer Reviewed
  • [Journal Article] Amino acids in conserved region II are crucial for substrate specificity, reaction velocity, and regioselectivity in transglucosylation of honeybee GH-13 α-glucosidases2012

    • Author(s)
      Ngiwsara L, Iwai G, Tagami T, Sato N, Nakai H, Okuyama M, Mori H, Kimura A
    • Journal Title

      Biosci Biotechnol Biochem

      Volume: 76(10) Pages: 1967-1974

    • DOI

      doi.org/10.1271/bbb.120473

    • Peer Reviewed
  • [Journal Article] Bacteroides thetaiotaomicron VPI- 5482 glycoside hydrolase family 66 homolog catalyzes dextranolytic and cyclization reactions2012

    • Author(s)
      Kim YM, Eiji Y, Kang MS, Nakai H, Saburi W, Okuyama M, Mori H, Funane K, Kim D, Kimura A
    • Journal Title

      FEBS J

      Volume: 279(17) Pages: 3185-3191

    • DOI

      doi:10.1111/j.1742-4658.2012.08698.x

    • Peer Reviewed
  • [Journal Article] Biochemical characterization of a cycloisomaltooligosaccharide glucanotransferase from Paenibacillus sp. 598K2012

    • Author(s)
      Suzuki R, Terasawa K, Kimura K, Fujimoto Z, Momma M, Kobayashi M, Kimura A, Funane K
    • Journal Title

      Biochim Biophys Acta

      Volume: 1824(7) Pages: 919-924

    • DOI

      doi:10.1016/j.bbapap.2012.04.001

    • Peer Reviewed
  • [Journal Article] α-Glucosidase-catalyzed novel reaction on 1,5-anhydrofructose, suggesting new metabolic pathway for production of glucose from starch2012

    • Author(s)
      Kim YM, Saburi W, Yu S, Nakai H, Maneesan J, Kang MS, Chiba S, Kim D, Okuyama M, Mori H, Kimura A
    • Journal Title

      J Biol Chem

      Volume: 287(27) Pages: 22441-22444

    • DOI

      doi:10.1074/jbc.C112.360909

    • Peer Reviewed
  • [Journal Article] Novel dextranase catalyzing cycloisomaltooligosaccharide-formation and its identification of catalytic amino acids and their functions using chemical rescue approach2012

    • Author(s)
      Kim YM, Kiso Y, Muraki T, Kang MS, Nakai H, Saburi W, Lang W, Kang HK, Okuyama M, Mori H, Suzuki R, Funane K, Suzuki N, Momma M, Fujimoto Z, Oguma T, Kobayashi M, Kim D, Kimura A
    • Journal Title

      J Biol Chem

      Volume: 287(24) Pages: 19927-19935

    • DOI

      doi:10.1074/jbc.M111.339036

    • Peer Reviewed
  • [Journal Article] Structural elucidation of dextran degradation mechanism by Streptococcus mutans dextranase belonging to glycoside hydrolase family 662012

    • Author(s)
      Suzuki N, Kim YM, Fujimoto Z, Momma M, Okuyama M, Mori H, Funane K, Kimura A
    • Journal Title

      J Biol Chem

      Volume: 287(24) Pages: 19916-19926

    • DOI

      doi:10.1074/jbc.M112.342444

    • Peer Reviewed
  • [Journal Article] Chemical constituents and free radical scavenging activity of corn pollen collected from Apis mellifera hives compared to floral corn pollen at Nan, Thailand2012

    • Author(s)
      Chantarudee A, Phuwapraisirisan P, Kimura K, Okuyama M, Mori H, Kimura A, Chanchao C
    • Journal Title

      BMC Complem Altern Med

      Volume: 12 Pages: 45-45

    • DOI

      doi:10.1186/1472-6882-12-45

    • Peer Reviewed
  • [Journal Article] Crystallization and preliminary crystallographic analysis of dextranase from Streptococcus mutans2011

    • Author(s)
      Suzuki N, Kim YM, Fujimoto Z, Momma M, Kang HK, Funane K, Okuyama M, Mori H,Kimura A
    • Journal Title

      Acta Crystallogr F

      Volume: F67 Pages: 1542-1544

    • DOI

      doi:10.1107/S1744309111038425

    • Peer Reviewed
  • [Journal Article] Bioengineering of Leuconostoc mesenteroides glucansucrases that gives selected bond formation for glucan synthesis and/or acceptor-product synthesis2011

    • Author(s)
      Kang HK, Kimura A, Kim D
    • Journal Title

      J Agric Food Chem

      Volume: 59(8) Pages: 4148-4155

    • DOI

      doi:10.1021/jf104629g

    • Peer Reviewed
  • [Journal Article] Novel quantitative method for the degree of branching in dextran2011

    • Author(s)
      Kim YM, Kimura A, Kim D
    • Journal Title

      Food Sci Biotechnol

      Volume: 20(2) Pages: 537-541

    • DOI

      doi:10.1007/s10068-011-0075-9

    • Peer Reviewed
  • [Journal Article] Optimal expression and characterization of a fusion enzyme having dextransucrase and dextranase activities2010

    • Author(s)
      Ryu HJ, Jin X, Lee JH, Woo HJ, Kim YM, Kim GJ, Seo ES, Kang HK, Kim J, Cho DL, Kimura A, Kim D
    • Journal Title

      Enzyme Microb Technol

      Volume: 47(5) Pages: 212-215

    • DOI

      doi:10.1016/j.enzmictec.2010.07.006

    • Peer Reviewed
  • [Journal Article] Rice Os4BGlu12 is a wound-induced β-glucosidase that hydrolyzes cell-wall-β-glucan-derived oligosaccharides and glycosides2010

    • Author(s)
      Opassiri R, Maneesan J, Akiyama T, Pomthong B, Jin S, Kimura A, Ketudat-Cairns JR
    • Journal Title

      Plant Sci

      Volume: 179(3) Pages: 273-280

    • DOI

      doi:10.1016/j.plantsci.2010.05.013

    • Peer Reviewed
  • [Journal Article] The first α-1,3-glucosidase from bacterial origin belonging to glycoside hydrolase family 312009

    • Author(s)
      Kang MS, Okuyama M, Mori H, Kimura A
    • Journal Title

      Biochimie

      Volume: 91(11-12) Pages: 1434-1442

    • DOI

      doi:10.1016/j.biochi.2009.07.018

    • Peer Reviewed
  • [Presentation] Enzymatic synthesis of acarviosyl- maltooligosaccharides and their inhibitory effects on α-glucosidase2012

    • Author(s)
      Tagami T, Tanaka Y, Mori H, Okuyama M, Kimura A
    • Organizer
      26th Internal Carbohydrate Symposium
    • Place of Presentation
      Madrid City Centre (Madrid, Spain)
    • Year and Date
      20120722-27
  • [Presentation] Amino acid residues in conserved region II involved in specific function of European honeybee (Apis mellifera) α-glucosidase isozymes2012

    • Author(s)
      Ngiwsara L, Iwai G, Mori H, Okuyama M, Kimura A
    • Organizer
      26th Internal Carbohydrate Symposium
    • Place of Presentation
      Madrid City Centre (Madrid, Spain)
    • Year and Date
      20120722-27
  • [Presentation] 糖質酵素の基礎研究とその展開2012

    • Author(s)
      木村淳夫
    • Organizer
      日本応用糖質科学会平成22年度大会(第59回)
    • Place of Presentation
      静岡県立コンベンションアーツセンターグランシップ (静岡)
    • Year and Date
      2012-09-17
  • [Presentation] Molecular mechanism of glycosylases catalyzing megalosaccharide production2012

    • Author(s)
      Mori H, Kim D, and Kimura A
    • Organizer
      BIT's 3rd Inaugural Symposium on Enzymes & Biocatalysis-2012, Xian Qujiang International Conference Center (Xian, China)
    • Year and Date
      2012-04-28
  • [Presentation] Enhancement of GH66 dextranase activity by truncation of N- and C-terminal variable regions, suggesting proenzyme-like activation2011

    • Author(s)
      Mori H, Nakai H, Kimura A
    • Organizer
      9th Carbohydrate Bioengineering Meeting
    • Place of Presentation
      Calouste Gulbenkian Foundation (Lisbon, Portugal)
    • Year and Date
      20110515-18
  • [Presentation] Molecular anatomy of α-1,6-glucoside-forming and -hydrolyzing enzymes, comprising megalosaccharide-producing enzyme2011

    • Author(s)
      Kim YM, Mori H, Kimura A
    • Organizer
      2011 CAB Agricultural Biotechnology Symposium on Agricultural Biomaterials for Next Generation
    • Place of Presentation
      Seoul National University (Seoul, Korea)
    • Year and Date
      2011-08-25
  • [Presentation] Molecular Mechanism of novel enzymes degrading and forming α-1,6-glucosidic linkages, including megalosaccharide production2011

    • Author(s)
      Kimura A
    • Organizer
      The 78th Annual Meeting and International Symposium in 2011 Annual Conference of Korean Society of Food Science and Technology
    • Place of Presentation
      Daegu Exhibition Convention Center (Daegu, Korea)
    • Year and Date
      2011-06-09
  • [Presentation] Putative role of Tyr227, Gln349 and Leu350 near catalytic amino acids of honeybee α-glucosidase III2010

    • Author(s)
      Mori H, Kimura A
    • Organizer
      4th Symposium on the Alpha-Amylase Family
    • Place of Presentation
      Smolenice Cattle (Smolenice, Slovakia)
    • Year and Date
      20100925-30
  • [Presentation] Biochemical characterization of a novel cycloisomaltooligosaccharide glucanotransferase (CITase) from Paenibacillus sp2010

    • Author(s)
      Suzuki R, Kimura A
    • Organizer
      598K. Plant Polysaccharide and Applied Glycoscience Workshop 2010
    • Place of Presentation
      Sasagawa Hall (Tokyo, Japan)
    • Year and Date
      20100729-31
  • [Presentation] Preliminary structure determination of water insoluble polysaccharide extracted from soil bacteria2009

    • Author(s)
      Wongchawalit J, Hashidoko Y, Okuyama M, Mori H, Chiba S, Kimura A
    • Organizer
      8th Carbohydrate Bioengineering Meeting
    • Place of Presentation
      2009 Hotel Continental Terme and Congress Hall (Naples, Italy)
    • Year and Date
      20090510-13
  • [Remarks]

    • URL

      http://hecate.general.hokudai.ac.jp/welcome/top-page-jpn.html

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Published: 2014-08-29  

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