Subtype specification of the olfactory sensory neurons in Drosophila by RUNX family transcription factor Lozenge

2011 Fiscal Year Final Research Report

• Project/Area Number: 21500302
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Neuroscience in general
• Research Institution: The University of Tokyo
• Principal Investigator: ENDO Keita 東京大学, 分子細胞生物学研究所, 助教 (40425616)
• Project Period (FY): 2009 – 2011
• Keywords: 神経クラス分化 / 嗅覚神経細胞 / Runx / Notch / エピジェネティック

Research Abstract

Olfactory sensory neurons in Drosophila melanogaster are diversified into 50 different classes, each expressing specific odorant receptors, and together contributing to detection and discrimination of a myriad of odorous chemicals in environment. To reveal the developmental mechanisms that generate this neuronal diversity, I analyzed the function of two molecules ; Runx-family transcription factor Lozenge and Drosophila homologue of Evi1 proto-oncogene Hamlet. I found that the precursor cells for the olfactory sensory neurons differentiate continuously while Lozenge level gradually decreases during development. This makes precursor cells that are differentiated at different time points receive different levels of Lozenge, thereby generating different classes of olfactory sensory neurons. I also found that Hamlet contributes to the process of a single precursor cell generating different classes of olfactory sensory neurons, by modifying the expression of the Notch-target genes through epigenetic mechanisms.

Research Products

• [Journal Article] Olfactory receptor neuronidentity is diversified by theDrosophila Evi1-Prdm16 homologHamlet that mediates chromatinmodification at Notch-target loci (2012)
  • Author(s): Endo, K., Karim, M. R., Taniguchi, H., Krejci, A., Kinameri, E., Siebert, M., Ito, K., Bray, S. J., and Moore, A. W
  • Journal Title: Nat. Neurosci Volume: 15 Pages: 224-233
  • DOI: doi:10.1038/nn.2998
• [Presentation] Olfactory Receptor Neuronsare Diversified by the Prdm ProteinHamlet that Mediates ChromatinModification at Notch-Target Loci (2011)
  • Author(s): 遠藤啓太
  • Organizer: 1st Asia-Pacific Drosophila Research Conference
  • Place of Presentation: Taipei
  • Year and Date: 20110500
• [Presentation] Olfactory Receptor Neuron Identity is Diversified by theDrosophila Evi1/Prdm16 HomologueHamlet that Mediates ChromatinModification at Notch-Target Loci (2011)
  • Author(s): 遠藤啓太
  • Organizer: 第44回発生生物学会大会
  • Place of Presentation: 沖縄
  • Year and Date: 20110500
• [Presentation] キイロショウジョウバエにおける嗅覚神経細胞のクラス分化機構 (2011)
  • Author(s): 遠藤啓太
  • Organizer: 第179回つくばブレインサイエンス(TBSA)・セミナー
  • Place of Presentation: つくば
  • Year and Date: 20110200
• [Presentation] Resetting of Notch signalingby hamlet ensures the repeated use ofthe Notch-mediated cell-fatespecification at each cell division todiversify the Drosophila olfactorysensory neurons (2010)
  • Author(s): 遠藤啓太
  • Organizer: 第33回日本分子生物学会年会・第83回日本生化学会大会合同大会
  • Place of Presentation: 神戸
  • Year and Date: 20101200
• [Presentation] キイロショウジョウバエ嗅覚神経細胞の多様性は細胞系譜特異的なNotchシグナルの修飾により獲得される (2010)
  • Author(s): 遠藤啓太
  • Organizer: 第33回日本神経科学大会・第53回日本神経化学会大会・第20回日本神経回路学会大会合同大会
  • Place of Presentation: 神戸
  • Year and Date: 20100900
• [Presentation] Sub-lineage-Specific Modification of Notch Transcriptional Regulation by Hamlet Generates Drosophila Olfactory Sensory Neuron Diversity (2010)
  • Author(s): 遠藤啓太
  • Organizer: 第43回発生生物学会大会
  • Place of Presentation: 京都
  • Year and Date: 20100700
• [Presentation] Combinatorial role of theEGFR signaling pathway and a RUNXfamily transcription factor Lozenge inthe subtype specification of the Drosophila olfactory sensory neurons (2009)
  • Author(s): 遠藤啓太
  • Organizer: 第32回日本神経科学大会
  • Place of Presentation: 名古屋
  • Year and Date: 20090900
• [Presentation] Lineage-specific modificationof Notch signaling by Hamletgenerates Drosophila olfactory neurondiversity (2009)
  • Author(s): 遠藤啓太
  • Organizer: 9th Japanese Drosophila Research Conference
  • Place of Presentation: 嬬恋
  • Year and Date: 20090700
• [Presentation] Combinatorial role of theEGFR signaling pathway and a RUNXfamily transcription factor Lozenge inthe subtype specification of the Drosophila olfactory sensory neurons (2009)
  • Author(s): 遠藤啓太
  • Organizer: 9th Japanese Drosophila Research Conference
  • Place of Presentation: 嬬恋
  • Year and Date: 20090700
• [Presentation] A Drosophila RUNX familytranscription factor, Lozenge, specifiesthe olfactory receptor neuron class in adoes-dependent manner
  • Author(s): 遠藤啓太
  • Organizer: CSHLmeeting "Neurobiology of Drosophila"
  • Place of Presentation: Cold Spring Harbor, New York
• [Remarks] 新聞報道(1)日刊工業新聞「理研と東大、嗅覚神経細胞をつくる仕組みを解明-iPS細胞分化に貢献」2012年1月9日
• [Remarks] (2)日経バイオテクONLINE「理研BSIと東大IMCB、嗅覚神経細胞分化でNotchとHamletがエピジェネティクス制御、12月24日に説明会」2011年12月28日
• [Remarks] (3)化学工業日報「嗅覚神経細胞の多様な分化/エピジェネティクスな現象/核内因子が標的遺伝子制御」2011年12月26日