Elucidation of molecular machinery for mouse forebrain compartmentalization

2011 Fiscal Year Final Research Report

• Project/Area Number: 21500333
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Nerve anatomy/Neuropathology
• Research Institution: National Center of Neurology and Psychiatry
• Principal Investigator: INOUE Takayoshi 独立行政法人国立精神・神経医療研究センター, 神経研究所・疾病研究第六部, 室長 (20370984)
• Co-Investigator(Renkei-kenkyūsha): INOUE Yukiko 国立精神・神経医療研究センター, 神経研究所・疾病研究第六部, 流動研究員 (30611777)
• Project Period (FY): 2009 – 2011
• Keywords: 神経実験形態学

Research Abstract

In the present research project, an original and efficient bacterial artificial chromosome-based enhancer bashing methodology was applied to clarify the detailed molecular machinery for mouse forebrain compartmentalization. As the results, a 110-bp fragment located approximately 40-kb upstream of the cell adhesion molecule Cdh6 gene transcription start site was confirmed to be necessary yet insufficient for the forebrain compartment specific expression. Importantly, together with the 110-bp fragment, an evolutionary conserved region that does not contain any forebrain specific master control gene Pax6 binding motifs was found to be synergistically sufficient for the Cdh6 expression, highlighting the value of combinatorial regulatory cascades in specifying the forebrain compartment as patterning basis for the elaborated cellular structure and function.

Research Products

• [Journal Article] Bacterial artificial chromosome as analytical basis for gene transcriptional machineries (2011)
  • Author(s): Asami J, Inoue YU, Terakawa YW, Egusa SF, Inoue T.
  • Journal Title: Transgenic Res Volume: 20 Pages: 913-924
  • DOI: DOI:10.1007/s11248-010-9469-3
  • Peer Reviewed
• [Journal Article] Cadherin-6 mediates axon-target matching in a non-image-forming visual circuit (2011)
  • Author(s): Osterhout JA, Josten N, Yamada J, Pan F, Wu SW, Nguyen PL, Panagiotakos G, Inoue YU, Egusa SF, Volgyi B, Inoue T, Bloomfield SA, Barres BA, Berson DM, Feldheim DA, Huberman AD
  • Journal Title: Neuron Volume: 71 Pages: 632-639
  • DOI: DOI:10.1016/j/neuron.2011.07.006
  • Peer Reviewed
• [Journal Article] Sox10-Venus mice : a new tool for real-time labeling of neural crest lineage cells and oligodendorocytes (2010)
  • Author(s): Shibata S, Yasuda A, Renault-Mihara F, Suyama S, Katoh H, Inoue T, Inoue YU, Nagoshi N, Sato M, Nakamura M, Akazawa C, Okano H.
  • Journal Title: Mol Brain Volume: 3 Pages: 31
  • DOI: DOI:10.1186/1756-6606-3-31
  • Peer Reviewed
• [Presentation] Gene regulatory patterns for Cdh6 expression identify divisible genetic compartments in the postnatal mouse cerebral cortex (2011)
  • Author(s): Inoue T, Asami J, Egusa SF, Inoue YU.
  • Organizer: 日本神経科学学会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2011-09-16
• [Presentation] Genetic labeling of mouse rhombomeres by Cadherin-6 :: EGFP-BAC transgenesis highlights the role of cadherins in hindbrain compartmentalization (2009)
  • Author(s): Inoue T.
  • Organizer: 日本分子生物学会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2009-12-11
• [Book] Bacterial Artificial Chromosomes(Chapter 7) Bacterial artificial chromosome-based experimental strategies in the field of developmental neuroscience (2011)
  • Author(s): Terakawa YW, Inoue YU, Asami J, Inoue T.
  • Total Pages: 103-118
  • Publisher: InTech
• [Remarks]
  • URL: http://www.ncnp.go.jp/nin/guide/r_diag/index.html