Functional analysis of SR protein, Mitsugumin 23.

2011 Fiscal Year Final Research Report

• Project/Area Number: 21500382
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Neurophysiology and muscle physiology
• Research Institution: Kyoto University
• Principal Investigator: NISHI Miyuki 京都大学, 大学院・薬学研究科, 研究員 (60183894)
• Project Period (FY): 2009 – 2011
• Keywords: カルシウム

Research Abstract

Mitsugumin 23(MG23) is a 23 kDa transmembrane protein localized to the sarcoplasmic/endoplasmic reticulum and nuclear membranes in a wide variety of cells. Single-particle three-dimensional reconstruction revealed that MG23 forms a large bowl-shaped complex equipped with a putative central core. After reconstitution into planar phospholipid bilayers, purified MG23 behaved as a voltage-dependent cation-conducting channel.

Research Products

• [Journal Article] Endogenously determined restriction of food intake overcomes excitation-contraction uncoupling in JP45KO mice with aging (2012)
  • Author(s): Delbono, O., Messi M. L., Wang, Z., Treves, S., Mosca, B., Bergamelli, L., Nishi, M., Takeshima, H., Shi H., Xue B. & Zorzato, F
  • Journal Title: Exp. Gerontol Volume: 4 Pages: 304-316
  • DOI: DOI:10.1016/j.exger.2012.01.004
  • Peer Reviewed
• [Journal Article] Mitsugumin 23 forms massive bowl-shaped assembly and cation-conducting channel (2011)
  • Author(s): Venturi, E., Mio, K., Nishi, M., Ogura, T., Moriya, T., Pitt, S. J., Okuda, K., Kakizawa, S., Sitsapesan R. Sato, C., & Takeshima, H.
  • Journal Title: Biochemistry Volume: 50 Pages: 2623-2632
  • DOI: DOI:10.1021/bi1019447
  • Peer Reviewed
• [Journal Article] TRIC-A Channels in Vascular Smooth Muscle Contribute to Blood Pressure Maintenance (2011)
  • Author(s): Yamazaki, D., Tabara Y., Kita S., Hanada, H., Komazaki, S., Naitou D., Mishima, A., Nishi, M., Yamamura, H., Yamamoto, S., Kakizawa, S., Miyachi, H. Yamamoto, S., Miyata, T., Kawano, Y., Kamide, K., Ogihara, T., Hata, A., Umemura, S., Soma, M., Takahashi,
  • Journal Title: Cell Metabolism Volume: 14 Pages: 231-241
  • DOI: DOI:10.1016/j.cmet.2011.05.011
  • Peer Reviewed
• [Journal Article] two ion-channels, TRIC-A and TRIC-B, masquerade as a single K+-channel (2010)
  • Author(s): Pitt, S. J., Park, KH, Nishi M., Urashima, T., Aoki, S., Yamazaki, D., Ma, J., Takeshima, H. & Sitsapesan R. Charade of the SR K+-channel
  • Journal Title: Biophys. J. Volume: 99 Pages: 417-426
  • DOI: DOI:10.1016/j.bpj.2010.04.051
  • Peer Reviewed
• [Journal Article] Ca2+ overload and sarcoplasmic reticulum instability in tric-a null skeletal muscle (2010)
  • Author(s): Zhao, X., Yamazaki, D., Park, KH., Komazaki, S., Tjondrokoesoemo, A., Nishi, M., Lin, P., Hirata, Y., Brotto, M., Takeshima, H & Ma, J.
  • Journal Title: J. Biol. Chem Volume: 285 Pages: 37370-37376
  • DOI: DOI:10.1074/jbc.M110.170084
  • Peer Reviewed
• [Journal Article] Facilitation of DNA damage-induced apoptosis by endplasmic reticulum protein mitsugumin23 (2010)
  • Author(s): Yamazaki, T., Sasaki, N., Nishi, M. & Takeshima, H.
  • Journal Title: Biochem. Biophys. Res. Commun Volume: 392 Pages: 196-200
  • DOI: DOI:10.1016/j.bbrc.2010.01.013
  • Peer Reviewed
• [Journal Article] MG53 constitutes a primary determinant of cardiac ischemic preconditioning (2010)
  • Author(s): Cao, CM., Zhang, Y., Weisleder, N., Ferrante, C., Wang, X., Lv, F., Zhang, Y., Song, R., Hwang, M., Jin, L., Guo, J., Peng, W., Li, G., Nishi, M., Takeshima, H., Ma, J. & Xiao, RP
  • Journal Title: Circulation Volume: 121 Pages: 2565-2574
  • DOI: DOI:10.1161/CIRCULATIONAHA.110.954628
  • Peer Reviewed
• [Journal Article] MG53 is an essential component of the acute membrane repair machinery (2009)
  • Author(s): Cai, C., Masumiya, H., Weisleder, N., Matsuda, N., Nishi, M., Hwang, M., Ko, JK., Lin, P., Thornton, A., Zhao, X., Pan, Z., Komazaki, S., Brotto, M., Takeshima, H & Ma, J.
  • Journal Title: Nature cell biol Volume: 11 Pages: 56-64
  • DOI: DOI:10.1038/ncb1812
  • Peer Reviewed
• [Journal Article] MG53 regulates membrane budding in muscle cells. (2009)
  • Author(s): Cai, C., Masumiya, H., Weisleder, N., Pan, Z., Nishi, M., Komazaki, S., Takeshima, H & Ma, J.
  • Journal Title: J. Biol. Chem Volume: 284 Pages: 3314-3322
  • DOI: DOI:10.1074/jbc.M808866200
  • Peer Reviewed
• [Journal Article] Membrane repair defects muscular dystrophy are linked to alterd interaction between MG53, caveolin-3 and dysferlin (2009)
  • Author(s): Cai, C., Weisleder, N., Ko, JK., Komazaki, S., Sunada, Y., Nishi, M., Takeshima, H. & Ma, J
  • Journal Title: J. Biol. Chem Volume: 284 Pages: 15894-15902
  • DOI: DOI:10.1074/jbc.M109.009589
  • Peer Reviewed
• [Journal Article] Essential role of the TRIC-B channel in Ca2+ handling of alveolar epithelial cells and inperinatal lung maturation (2009)
  • Author(s): Yamazaki, D., Komazaki, S., Nakanishi, H., Mishima, A., Nishi, M., Yazawa, M., Yamazaki, T., Taguchi, R & Takeshima H.
  • Journal Title: Development Volume: 136 Pages: 2355-2361
  • DOI: DOI:10.1242/dev.036798
  • Peer Reviewed
• [Presentation] Up regulation of calcium influx via Cav11 in skeletal muscle fibers from JP-45 calsequestrin 1 double knock out mice (2012)
  • Author(s): Barbara Mosca, Leda Bergamelli, Mirko Vukcevic, Susan Treves, Cecilia Paoloni, Miyuki Nishi, Hiroshi Takeshima, Feliciano Protasi & Francesco Zorzato
  • Organizer: Biophysical Society 56^<th> Annual meeting
  • Place of Presentation: San Diego, California(USA)
  • Year and Date: 20120225-29
• [Presentation] Rebbeca Sitsapesan, Chikara sato & Hiroshi Takeshima. Mitsugumin23, a protein associated with intracellular calcium stores, behaves as an ion-channel that can conduct calcium (2011)
  • Author(s): Kazuhiro Mio, Miyuki Nishi, Toshihiko Ogura, Toshio Moriya, Samantha J. Pitt, Kazutaka Okuda
  • Organizer: Biophysical Society 55^<th> Annual meeting
  • Place of Presentation: Baltimore, Maryland(USA)
  • Year and Date: 20110305-09
• [Presentation] Aya Mishima, Miyuki Nishi, Takahiro Iwamoto & Hiroshi Takeshima. Role of TRIC-A channel in circulatory function (2010)
  • Author(s): Daiju Yamazaki, Satomi Kota, Shinji Komazaki
  • Organizer: Biophysical Society 54^<th> Annual meeting
  • Place of Presentation: San Francisco, California(USA)
  • Year and Date: 20100220-24
• [Remarks]
  • URL: http://www.pharm.kyoto-u.ac.jp/biochem/