2011 Fiscal Year Final Research Report
Effect of mechanical stress to ligament/tendon fibroblast under 3D culture environment
| Project/Area Number |
21500399
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Biomedical engineering/Biological material science
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| Research Institution | Hokkaido University |
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Principal Investigator |
MAJIMA Tokifumi 北海道大学, 大学院・医学研究科, 特任教授 (30241334)
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| Co-Investigator(Kenkyū-buntansha) |
SAWAGUCHI Naohiro 北海道大学, 北海道大学病院, 助教 (80435982)
KASAHARA Yasuhiko 北海道大学, 北海道大学病院, 助教 (00581927)
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| Project Period (FY) |
2009 – 2011
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| Keywords | バイオメカニクス / 腱・靭帯 / 線維芽細胞 / リモデリング |
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| Research Abstract |
Tissue engineering techniques using biodegradable three-dimensional (3D) scaffolds with cultured cells offer more potential alternatives for the treatment of severe ligament and tendon injuries. In tissue engineering, one of the crucial roles of 3D scaffolds is to provide a temporary template with the biomechanical characteristics of the native extracellular matrix (ECM) until the regenerated tissue matures. The purpose of the present study was to assess the effect of various cyclic mechanical stresses on cell proliferation and ECM production in a 3D scaffold made from chitosan and hyaluronan for ligament and tendon tissue engineering.
Multidimensional cyclic mechanical strain to mimic the physiological condition in vivo has the potential to improve or accelerate tissue regeneration in ligament and tendon tissue engineering using 3D scaffolds in vitro.
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