2011 Fiscal Year Final Research Report
Construction of a stimulation-responsive gene regulation system and its application for gene therapy
| Project/Area Number |
21500403
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Biomedical engineering/Biological material science
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| Research Institution | University of Toyama |
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Principal Investigator |
OGAWA Ryohei 富山大学, 大学院・医学薬学研究部(医学), 講師 (60334736)
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| Co-Investigator(Kenkyū-buntansha) |
KAGIYA Go 北里大学, 医療衛生学部, 講師 (30524243)
ZHAO Qing-li 富山大学, 大学院・医学薬学研究部(医学), 助教 (90313593)
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| Project Period (FY) |
2009 – 2011
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| Keywords | 生体制御 / 治療 |
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| Research Abstract |
We have established a retrovirus vector system to stably introduce a gene cassette consisting of a constructed radiation responsive promoter and a gene of interest. In a cell line expressing the luciferase gene, we showed expression of the luciferase gene was possible with radiation. In addition, with an established cell line expressing the fcy :: fur gene, a suicide gene, it was possible to enhance cell killing effect when radiation was delivered, showing radiation dependent suicide gene therapy simulation in vitro. Furthermore, using another set of cis-elements, we successfully obtained radiation responsive promoters effectively working in a prostate cancer cell line. Moreover, we found that sonication changed expressions of microRNAs that could be involved in response of a cell to ultrasound. In addition, we showed that target sequences of microRNAs that decrease their expressions by sonication could be a more fine-tuned ultrasound mediated gene regulation system when combined with an ultrasound responsive promoter.
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[Journal Article] Regulation of gene expression in retrovirus vectors by X-ray and proton beam radiation with artificially constructed promoters2012
Author(s)
Ogawa, R., A. Morii, A. Watanabe, Z. G. Cui, G. Kagiya, S. Fukuda, K. Kume, T. Hasegawa, M. Hatashita, H. Izumi and T. Ishimoto
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Journal Title
J Gene Med
Volume: 14
Pages: 316-327
DOI
Peer Reviewed
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