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2011 Fiscal Year Final Research Report

Creation of novel matrix for cell culture using CBMand chitin

Research Project

  • PDF
Project/Area Number 21500423
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Biomedical engineering/Biological material science
Research InstitutionTokyo Institute of Technology

Principal Investigator

NAKAMURA Satoshi  東京工業大学, 大学院・生命理工学研究科, 教授 (50227899)

Research Collaborator FUKAGAWA Satoko  東京工業大学, 大学院・生命理工学研究科, 学生
Project Period (FY) 2009 – 2011
Keywords再生医工学材料
Research Abstract

Chitinase J from alkaliphilic Bacillus sp. contained a catalytic domain, a fibronectin type III-like domain(FnIIID) and a chitin-binding domain(ChBD). A peptide sequence Arg-Gly-Asp(RGD) sequence locates within fibronectin, an extracellular matrix molecule, and interacts with cells via a cell surface receptor called integrin. Several kinds of RGD-fused FnIIID/ChBD and ChBD proteins were prepared and characterized. The fusion proteins indicated chitin-binding, integrin-binding and cell adhesive activities.

  • Research Products

    (18 results)

All 2012 2011 2010 2009 Other

All Journal Article (10 results) (of which Peer Reviewed: 9 results) Presentation (2 results) Book (5 results) Remarks (1 results)

  • [Journal Article] Mutational analysis of a CBM family 5 chitin-binding domain of an alkaline chitinase from Bacillus sp2012

    • Author(s)
      F. Uni, S. Lee, R. Yatsunami, T. Fukui, and S. Nakamura
    • Journal Title

      Biosci. Biotechnol. Biochem

      Volume: 76 Pages: 530-535

    • Peer Reviewed
  • [Journal Article] Mechanism of the thermal inactivation of a MBP-fused heparinase I : Biochemical investigations and kinetic modeling2011

    • Author(s)
      S. Chen, F. Ye, H. Zhao, Y. Chena, Y. Chen, S. Nakamura, F. Arisaka and X. Xing
    • Journal Title

      Biotechnol. Bioeng

      Volume: 108 Pages: 1841-1851

    • Peer Reviewed
  • [Journal Article] alcium-dependent xylan-binding domain of alkaline xylanase from alkaliphilic Bacillus sp. strain 41M-12011

    • Author(s)
      R. Yazawa, J. Takakura, T. Sakata, Ihsanawati, R. Yatsunami, T. Fukui, T. Kumasaka, N. Tanaka and S. Nakamura, A
    • Journal Title

      Biosci. Biotechnol. Biochem

      Volume: 75 Pages: 379-381

    • Peer Reviewed
  • [Journal Article] Improvement of alkaliphily of thermostable GH family 10 xylanase from Thermotoga maritima by directed evolution2010

    • Author(s)
      W. Tsukimura, K. Watanabe, C. Morokuma, R. Yatsunami, T. Fukui and S. Nakamura
    • Journal Title

      Jpn. Soc. Extr.

      Volume: 9 Pages: 15-18

    • Peer Reviewed
  • [Journal Article] Gene expression and characterization of a novel GH family 18 chitinase from extremely halophilic archaeon Halobacterium salinarum NRC-12010

    • Author(s)
      R. Yatsunami, M. Sato, K. Orishimo, Y. Hatori, Y. Zhang, T. Takashina, T. Fukui and S. Nakamura
    • Journal Title

      pn. Soc. Extr.

      Volume: 9 Pages: 19-24

    • Peer Reviewed
  • [Journal Article] Characterization of a haloarchaeal GH family 18 chitinase with additional acidic amino acids on its protein surface2010

    • Author(s)
      Y. Zhang, R. An, R. Yatsunami, M. Sato, K. Orishimo, Y. Hatori, T. Fukui and S. Nakamura
    • Journal Title

      Jpn. Soc. Extr.

      Volume: 9 Pages: 72-74

    • Peer Reviewed
  • [Journal Article] Improvement of alkaliphily of Bacillus alkaline xylanase by introducing amino acid substitutions both on catalytic cleft and protein surface, Biosci2009

    • Author(s)
      H. Umemoto, Ihsanawati, M. Inami, R. Yatsunami, T. Fukui, T. Kumasaka, N. Tanaka and S. Nakamura
    • Journal Title

      Biotechnol. Biochem.

      Volume: 73 Pages: 965-967

    • Peer Reviewed
  • [Journal Article] Additional carbohydrate-binding modules enhance the insoluble substrate hydrolytic activity of beta-1, 3-glucanase from alkaliphilic Nocardiopsis sp. F962009

    • Author(s)
      N. Koizumi, S. Masuda, K. Maeda, Y. Isoda, R. Yatsunami, T. Fukui and S. Nakamura
    • Journal Title

      Biosci. Biotechnol. Biochem.

      Volume: 73 Pages: 1078-1082

    • Peer Reviewed
  • [Journal Article] Analysis of functional domains and improvement of alkaliphily of an alkaline xylanase on the basis of its three-dimensional structure2009

    • Author(s)
      H. Umemoto, R. Yazawa, J. Takakura, R. Yatsunami, T. Fukui and S. Nakamura
    • Journal Title

      J. Appl. Glycosci.

      Volume: 57 Pages: 145-150

    • Peer Reviewed
  • [Journal Article] Role of exposed aromatic residues in substrate-binding of CBM family 5 chitin-binding module of alkaline chitinase2009

    • Author(s)
      F. Uni, S. Lee, R. Yatsunami, T. Fukui and S. Nakamura
    • Journal Title

      Nucleic Acids Symp. Ser.

      Volume: 53 Pages: 311-312

  • [Presentation] Mutational analysis of a CBM family 5 chitin-binding domain of alkaline chitinase2010

    • Author(s)
      F. Uni, S. Lee, R. Yatsunami, T. Fukui and S. Nakamura
    • Organizer
      TOCAT6
    • Place of Presentation
      Sapporo
    • Year and Date
      20100618-23
  • [Presentation] ヘミセルロースの有効利用に向けたアルカリキシラナーゼの機能向上2010

    • Author(s)
      中村聡
    • Organizer
      日本農芸化学会2010年度大会
    • Place of Presentation
      東京
    • Year and Date
      2010-03-30
  • [Book] Extremophiles Handbook2011

    • Author(s)
      S. Nakamura, K. Nakasone and T. Takashina
    • Total Pages
      363-381
    • Publisher
      Genetics and genomics of triangular disc-shaped halophilic archaeon Haloarcula japonica strain TR-1
  • [Book] ソフトマテリアルの応用展開(西敏夫・編),シーエムシー,大阪2010

    • Author(s)
      三原久和,中村聡,小畠英理
    • Total Pages
      3-15
    • Publisher
      ソフトマテリアルとしてのタンパク質・ペプチド
  • [Book] Biotechnology of Lignocellulose Degradation and Biomass Utilization2009

    • Author(s)
      Y. Zhang, R. Yatsunami, Y. Hatori, M. Sato, K. Orishimo, T. Fukui and S. Nakamura
    • Total Pages
      442-447
    • Publisher
      Halotolerancy and organic-solvent tolerancy of haloarchaeal chitinase
  • [Book] Biotechnology of Lignocellulose Degradation and Biomass Utilization2009

    • Author(s)
      W. Tsukimura, K. Watanabe, C. Morokuma, Ihsanawati, R. Yatsunami, T. Fukui, T. Kumasaka, N. Tanaka and S. Nakamura
    • Total Pages
      423-427
    • Publisher
      Improvement of alkaliphily of family 10 xylanase from hyperthermophile Thermotoga maritima
  • [Book] Biotechnology of Lignocellulose Degradation and Biomass Utilization2009

    • Author(s)
      R. Yazawa, J. Takakura, T. Sakata, H. Miyakubo, Ihsanawati, H. Umemoto, R. Yatsunami, T. Fukui, T. Kumasaka, N. Tanaka, and S. Nakamura
    • Total Pages
      448-454
    • Publisher
      Mutational analysis of CBM family 36 xylan-binding domain of alkaline xylanase
  • [Remarks]

    • URL

      http://www.nakamura.bio.titech.ac.jp/

URL: 

Published: 2013-07-31  

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