2011 Fiscal Year Final Research Report
Creation of novel matrix for cell culture using CBMand chitin
Project/Area Number |
21500423
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
Biomedical engineering/Biological material science
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Research Institution | Tokyo Institute of Technology |
Principal Investigator |
NAKAMURA Satoshi 東京工業大学, 大学院・生命理工学研究科, 教授 (50227899)
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Research Collaborator |
FUKAGAWA Satoko 東京工業大学, 大学院・生命理工学研究科, 学生
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Project Period (FY) |
2009 – 2011
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Keywords | 再生医工学材料 |
Research Abstract |
Chitinase J from alkaliphilic Bacillus sp. contained a catalytic domain, a fibronectin type III-like domain(FnIIID) and a chitin-binding domain(ChBD). A peptide sequence Arg-Gly-Asp(RGD) sequence locates within fibronectin, an extracellular matrix molecule, and interacts with cells via a cell surface receptor called integrin. Several kinds of RGD-fused FnIIID/ChBD and ChBD proteins were prepared and characterized. The fusion proteins indicated chitin-binding, integrin-binding and cell adhesive activities.
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Research Products
(18 results)
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[Book] Extremophiles Handbook2011
Author(s)
S. Nakamura, K. Nakasone and T. Takashina
Total Pages
363-381
Publisher
Genetics and genomics of triangular disc-shaped halophilic archaeon Haloarcula japonica strain TR-1
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[Book] Biotechnology of Lignocellulose Degradation and Biomass Utilization2009
Author(s)
R. Yazawa, J. Takakura, T. Sakata, H. Miyakubo, Ihsanawati, H. Umemoto, R. Yatsunami, T. Fukui, T. Kumasaka, N. Tanaka, and S. Nakamura
Total Pages
448-454
Publisher
Mutational analysis of CBM family 36 xylan-binding domain of alkaline xylanase
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