2011 Fiscal Year Final Research Report
Studies on the formation mechanism and function of prevascular network structures within multi-layered cell-sheet constructs
| Project/Area Number |
21500433
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Biomedical engineering/Biological material science
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| Research Institution | Tokyo Women's Medical University |
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Principal Investigator |
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| Project Period (FY) |
2009 – 2011
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| Keywords | 組織工学 / 再生医療 / 細胞シート / 血管新生 / 三次元積層化共培養 / 血管内皮細胞ネットワーク形成 |
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| Research Abstract |
Using a cell-sheet staking 3-D co-culture system, the mechanisms of prevascular network formation within layered cell-sheet and neovasvularization by engrafted prevascular cell-sheets were investigated. Two human genes encoding secreted glycoprotein and intracellular protein, expressed in endothelial cells (Ecs), were newly identified. Especially, EC network formation on Matrigel was inhibited by treating the cells with siRNA for the latter gene, indicating that this gene might be one of the regulators for prevascular network formation. On the other hand, the productions of both placenta growth factor (PIGF) and matrix metalloproteinase-9 (MMP-9) significantly increased in the prevascular cell-sheets. Therefore, these soluble proteins might play an important role in the functional anastomosis after subcutaneous transplantation.
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