2011 Fiscal Year Final Research Report
A preliminary study for clinical application of the anti-tumor enhancement effects using low-intensity ultrasound under the treatment with molecular target drugs.
| Project/Area Number |
21500450
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Medical systems
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| Research Institution | Nara Medical University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
FUJIMOTO Shinichi 奈良県立医科大学, 医学部, 教授 (70209097)
MIZUNO Reiko 奈良県立医科大学, 医学部, 講師 (80398437)
OTA Ichiro 奈良県立医科大学, 医学部, 助教 (00326323)
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| Project Period (FY) |
2009 – 2011
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| Keywords | 超音波医科学 |
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| Research Abstract |
Purpose : Molecular target drugs are recognized as having anti-tumor effects against specific target molecules. Although these drugs are less toxic for major organs, representing hematological and gastrointestinal toxicities, when compared with conventional cytotoxic anti-tumor drugs, the anti-tumor intensities still have been in unsatisfactory levels with a single use of these drugs. The investigators already showed the enhancement of anti-tumor effects by the combination of anti-human CD20 antibody, rituximab and low-intensity ultrasound in a B-cell lymphoma cell line, and the same results were also confirmed by the preliminary experiments in the present study. As a new study design, we tried whether anti-Epidermal Growth Factor Receptor(EGFR) antibody, cetuximab(C-mab) have a potential power to enhance anti-tumor effects followed by ultrasound exposure using EGFR expressing head and neck squamous cell carcinoma cell lines(HSC-3, HSC-4) in vitro.
Methods : After 12 hours duration for
… More
1x10^6 number of HSC-3 or HSC-4 cell culture, C-mab was concentrated and administered under the condition of 100nM into cell medium. Thirty minutes after administration of C-mab, ultrasound was exposed to the dish under the condition of pulse repetition frequency(PRF) 1Hz, intensity 0.5 W/cm^2(=output intensity of 0.38 W/cm^2), duration 1 minute.
Results and Discussion : Both of growth suppression and apoptosis induction effect in head administration followed by ultrasound exposure compared with either C-mab administration or ultrasound exposure alone. These results suggest that the anti-tumor effect may possibly be enhanced by the combination of molecular target drug and low-intensity ultrasound in EGFR expressing head and neck squamous cell carcinoma cell lines, as well as that has been already observed in a B-cell lymphoma cell line. Based on the present study, the further investigation would be planned toward the possibility if the ultrasound exposure be able to enhance the anti-tumor effect by C-mab in vivo. Less
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