2011 Fiscal Year Final Research Report
(-)-epigallocatechin-3-gallate protects against neuronal cell death and improves cerebralfunction after traumatic brain injury in rats
| Project/Area Number |
21500803
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Eating habits, studies on eating habits
|
|---|
| Research Institution | Kinki University |
|---|
Principal Investigator |
SATOU Takao 近畿大学, 医学部・附属病院, 教授 (70162443)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
ITOH Tatsuki 近畿大学, 医学部, 講師 (40330245)
|
|---|
| Project Period (FY) |
2009 – 2011
|
| Keywords | 脳外傷 / 緑茶飲料 / 神経保護作用 / 酸化ストレス / EGCG |
|---|
| Research Abstract |
A major component of green tea, a widely consumed beverage, is(-)-epigallocatechin gallate(EGCG), which has strong antioxidant properties. In this study, we investigated the effects of EGCG on cerebral function and morphology following traumatic brain injury(TBI). Six-week-old male Wistar rats that had access to normal drinking water, or water containing 0.1%(w/v) EGCG ad libitum, received TBI with a pneumatic controlled injury device at 10 weeks of age. Immunohistochemistry and lipid peroxidation studies revealed that at 1, 3 and 7 days post-TBI, the number of 8-OHdG-, 4-HNE-and ssDNA-positive cells, and the levels of MDA around the damaged area after TBI, significantly decreased in the EGCG treatment group compared with the water group. In addition, there was a significant increase in the number of surviving neuronal cells and an improvement in cerebral dysfunction after TBI in the EGCG treatment group compared with the water group. In summary, consumption of green tea may be an effective therapy for TBI patients.
|
