2011 Fiscal Year Final Research Report
Development of methods for analyzing fungal metabolites by using a portable ion mobility spectrometer under atmospheric pressure
Project/Area Number |
21500988
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
Cultural property science
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Research Institution | Nara Women's University |
Principal Investigator |
|
Co-Investigator(Renkei-kenkyūsha) |
KIUCHI Masato 独立行政法人産業技術総合研究所, ユビキタスエネルギー研究部門, 主任研究員 (50356862)
|
Project Period (FY) |
2009 – 2011
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Keywords | カビ臭 / 固相マイクロ抽出 / GCMS / 文化財 / 環境保全技術 / イオンモビリティースペクトロメトリー / MVOC / 微生物由来揮発性有機化合物 |
Research Abstract |
Fungal odor analysis in early growth stages is important for the conservation of cultural properties. The analytical methods for detecting fungal species and the stage of growth were developed using a distinctive fungal odor[Takeuchi et al, Surf. Interface Anal., 44(6), 694-698(2012)]. Microbial volatile organic compounds were extracted by solid space microextaction, followed by GC/MS analysis. While the fungi were growing, the amount of alcohols, ketones and aldehydes as MVOCs emitted increased. The detection of sesquiterpenes was useful for determining fungal species. The prototype of solid-phase microextraction inlet system available in an ion mobility spectrometer(IMS) was manufactured. Software programs were also made for identifying fungal species by IMS spectra analysis of the odor.
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