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2011 Fiscal Year Final Research Report

Development of methods for analyzing fungal metabolites by using a portable ion mobility spectrometer under atmospheric pressure

Research Project

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Project/Area Number 21500988
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Cultural property science
Research InstitutionNara Women's University

Principal Investigator

TAKEUCHI Takae  奈良女子大学, 理学部, 准教授 (80201606)

Co-Investigator(Renkei-kenkyūsha) KIUCHI Masato  独立行政法人産業技術総合研究所, ユビキタスエネルギー研究部門, 主任研究員 (50356862)
Project Period (FY) 2009 – 2011
Keywordsカビ臭 / 固相マイクロ抽出 / GCMS / 文化財 / 環境保全技術 / イオンモビリティースペクトロメトリー / MVOC / 微生物由来揮発性有機化合物
Research Abstract

Fungal odor analysis in early growth stages is important for the conservation of cultural properties. The analytical methods for detecting fungal species and the stage of growth were developed using a distinctive fungal odor[Takeuchi et al, Surf. Interface Anal., 44(6), 694-698(2012)]. Microbial volatile organic compounds were extracted by solid space microextaction, followed by GC/MS analysis. While the fungi were growing, the amount of alcohols, ketones and aldehydes as MVOCs emitted increased. The detection of sesquiterpenes was useful for determining fungal species. The prototype of solid-phase microextraction inlet system available in an ion mobility spectrometer(IMS) was manufactured. Software programs were also made for identifying fungal species by IMS spectra analysis of the odor.

  • Research Products

    (20 results)

All 2012 2011 2010 2009 Other

All Journal Article (4 results) (of which Peer Reviewed: 3 results) Presentation (14 results) Book (1 results) Remarks (1 results)

  • [Journal Article] Analysis of volatile metabolites emitted by soilderived fungi using head space solid-phase microextraction/gas chromatography/mass spec-trometry : I. Aspergillus fumigatus, Aspergillus nidulans, Fusarium solani and Penicillium paneum2012

    • Author(s)
      Takae Takeuchi, Tomoko Kimura, Haruna Tanaka, Sachiyo Kaneko, Shoko Ichii, Masato Kiuchi and Takahito Suzuki
    • Journal Title

      Surf. Interface Anal

      Volume: 44(6) Pages: 694-698

    • DOI

      DOI:10.1002/sia.4870

    • Peer Reviewed
  • [Journal Article] Fungal Odor Analysis for Conservation of Cultural Properties2012

    • Author(s)
      T. Takeuchi, M. Kiuchi and T. Suzuki
    • Journal Title

      J. Japan Associa-tion on Odor Environment

      Volume: 43(3)

  • [Journal Article] Effects of the silicon core structures on the hole mobility of star-shaped oligothiophenes2010

    • Author(s)
      J. Ohshita, Y. Hatanaka, S. Matsui, T. Mizumo, Y. Kunugi, Y. Honsho, A. i Saeki, S. Seki, J. Tibbelin, H. Ottosson and T. Takeuchi
    • Journal Title

      Dalton Transactions.

      Volume: 39 Pages: 9314-9320

    • DOI

      DOI:10.1039/C0DT00224K

    • Peer Reviewed
  • [Journal Article] Distinct features of matrix-assisted 6μm infrared laser desorption/ionization mass spectrometry in biomolecular snalysis2009

    • Author(s)
      M. Tajiri, T. Takeuchi and Y. Wada
    • Journal Title

      Anal. Chem.

      Volume: 81(16) Pages: 6750-6755

    • DOI

      DOI:10.1021/ac900695q

    • Peer Reviewed
  • [Presentation] The Effect of Incubation Temperature on Microbial Volatile Organic Compounds Emitted by Aspergillus fumigatus and Penicillium paneum2012

    • Author(s)
      Takae Takeuchi, Tomoko Kimura, Shoko Ichii, Masato Kiuchi, Sachiyo Kaneko and Takahito Suzuki
    • Organizer
      60^<th> ASMS Conference on Mass Spectrometry and Allied Topics
    • Place of Presentation
      Vancouver(Canada)
    • Year and Date
      2012-05-21
  • [Presentation] イオンモビリティースペクトロメトリーと質量分析法による土壌由来カビ代謝物の分析2012

    • Author(s)
      櫟彰子, 木村知子, 大岩さゆり, 鈴木孝仁, 木内葉子, 竹内孝江
    • Organizer
      日本化学会第92春季年会
    • Place of Presentation
      横浜
    • Year and Date
      2012-03-26
  • [Presentation] 簡易な検出器でカビ臭を解析カビ種と生育状況を推測するソフトウェアを開発-IMSによる土壌由来カビ検出データベースの構築-2012

    • Author(s)
      竹内孝江, 櫟彰子, 中村義隆, 紅朋浩, 菅井俊樹, 本城国明, 岩松雅子, 木内正人
    • Organizer
      次世代ナノテクフォーラム2012
    • Place of Presentation
      大阪
    • Year and Date
      2012-02-09
  • [Presentation] 微生物由来揮発性有機化合物の固相マイクロ抽出ガスクロマトグラフィー質量分析による真菌類のキャラクタリゼーション2011

    • Author(s)
      田中春菜, 木村知子, 金子幸代, 鈴木孝仁, 竹内孝江
    • Organizer
      日本化学会第91春季年会
    • Year and Date
      20110300
  • [Presentation] MSフラグメンテーションの基礎理論-準平衡理論(QET)-2011

    • Author(s)
      竹内孝江
    • Organizer
      第3回LC/MSワークショップ
    • Place of Presentation
      掛川
    • Year and Date
      2011-10-27
  • [Presentation] ポリジメチルシロキサンを用いたカビ臭物質濃縮のための固相マイクロ抽出プローブの開発-IMSによる文化財環境モニターへの適用-2011

    • Author(s)
      竹内孝江, 本城国明, 岩松雅子, 櫟彰子
    • Organizer
      第15回ケイ素化学協会シンポジウム
    • Place of Presentation
      須磨
    • Year and Date
      2011-10-21
  • [Presentation] イオンモビリティースペクトロメトリーと質量分析による土壌由来カビAspergillus nidulansの揮発性代謝物の同定2011

    • Author(s)
      櫟彰子, 菅井俊樹, 木村知子, 田中春菜, 金子幸代, 木内正人, 鈴木孝仁, 竹内孝江
    • Organizer
      第59回質量分析総合討論会
    • Place of Presentation
      大阪
    • Year and Date
      2011-09-15
  • [Presentation] Physiological activity of microbial volatile organic compounds(MVOCs) as a growth regulator in the soil-derived fungal organisms2011

    • Author(s)
      Sachiyo Kaneko, Haruna Tanaka, Tomoko, Kimura, Takae Takeuchi, Masato Kiuchi, Shin-ichi Iwaguchi, Koji Yokoyama, Takahito Suzuki
    • Organizer
      International Union of Microbiological Societies 2011 Congress
    • Place of Presentation
      Sapporo, Japan
    • Year and Date
      2011-09-09
  • [Presentation] 招待講演「微生物由来揮発性有機化合物の測定法2011

    • Author(s)
      竹内孝江
    • Organizer
      JAIMAコンファレンス・室内環境学会主催講演会
    • Place of Presentation
      幕張
    • Year and Date
      2011-09-08
  • [Presentation] Analysis of Volatile Metabolites Emitted by Soil Fungi Using Head Space Solid-Phase Microextraction GC/MS and Ion Mobility Spectrometry2011

    • Author(s)
      T. Takeuchi, T. Kimura, H. Tanaka, S. Kaneko, S. Ichii, M. Kiuchi and T. Suzuki
    • Organizer
      59^<th> ASMS Conference on Mass Spectrometry and Allied Topics
    • Place of Presentation
      Denver(USA)
    • Year and Date
      2011-06-06
  • [Presentation] Characterization of the Soil-Derived Fungi Based on Mass Spectrometric Analysis of Their Microbial Volatile Organic Compounds2011

    • Author(s)
      T. Takeuchi, H. Tanaka, T. Kimura, S. Ichii, M. Iwamatsu, S. Kaneko, M. Kiuchi and T. Suzuki
    • Organizer
      11^<th> International Symposium on Atom Level Characterization for New Materials and Devices' 11(ALC' 11)
    • Place of Presentation
      Seoul(Korea)
    • Year and Date
      2011-05-23
  • [Presentation] カビの他感作用物質としての2-pentadecanone2010

    • Author(s)
      金子幸代, 田中春菜, 竹内孝江, 木内正人, 岩口伸一, 鈴木孝仁
    • Organizer
      日本植物学会第74回大会
    • Place of Presentation
      愛知
    • Year and Date
      2010-09-09
  • [Presentation] 招待講演「マススペクトロメトリーにおける気相イオン化学と反応ダイナミックス」2010

    • Author(s)
      竹内孝江
    • Organizer
      原子衝突研究協会第35回年会
    • Place of Presentation
      奈良
    • Year and Date
      2010-08-09
  • [Presentation] 招待講演「文化財環境モニターのための小型臭気センサーの開発」2010

    • Author(s)
      竹内孝江
    • Organizer
      日本化学会第90春季年会
    • Place of Presentation
      大阪
    • Year and Date
      2010-03-29
  • [Book] Microbial volatile organic compounds(MVOCs) and their presumed physiological role for growth regulation in the soil-derived fungal organisms", Proceedings of JSPS-IIAS Joint International Symposium on Conservation Technology for Cultural Heritage2009

    • Author(s)
      S. Kaneko, H. Tanaka, T. Kimura, T. Takeuchi, M. Kiuchi, S. Iwaguchi, K. Yokoyama and T. Suzuki
    • Total Pages
      46-51
    • Publisher
      International Institute for Advanced Studies, Kyoto
  • [Remarks]

    • URL

      http://koto10.nara-wu.ac.jp/Profiles/5/0000420/profile.html

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Published: 2013-07-31  

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