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2011 Fiscal Year Final Research Report

Functional analysis of CUL4/DDB1 ubiquitin E3 ligase in DNA damage response

Research Project

  • PDF
Project/Area Number 21510055
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Risk sciences of radiation/Chemicals
Research InstitutionKanazawa University

Principal Investigator

WAKASUGI Mitsuo  金沢大学, 薬学系, 助教 (80345595)

Co-Investigator(Kenkyū-buntansha) MATSUNAGA Tsukasa  金沢大学, 薬学系, 教授 (60192340)
Project Period (FY) 2009 – 2011
Keywordsゲノム / シグナル伝達 / DNA損傷応答 / ユビキチン
Research Abstract

The Cul4/DDB1 complex is a recently identified culling-RING ubiquitin ligase, which regulates DNA repair, DNA replication and transcription. We analyzed the potential functions of DDB1 in the responses to various DNA damaging agents using a conditional knockout DT40 cells. Furthermore, we found that the interaction between DDB1 and Cul4 is absolutely required for cell viability, suggesting an essential role of DDB1 as a component of Cul4/DDB1 ubiquitin E3 ligase.

  • Research Products

    (23 results)

All 2012 2011 2010 2009 Other

All Journal Article (7 results) (of which Peer Reviewed: 6 results) Presentation (14 results) Remarks (1 results) Patent(Industrial Property Rights) (1 results)

  • [Journal Article] Establishment of a microplate for mattedcell-based immunoassay for rapid analysis of nucleotide excision repair ability in human primary cells2012

    • Author(s)
      Nishinaga M, Kurata R, Onishi K, Kuriyama K, Wakasugi M, Matsunaga T.
    • Journal Title

      Photochem. Photobiol

      Volume: 88 Pages: 356-62

    • DOI

      doi:10.1111/j.1751-1097.2012.01073.x

    • Peer Reviewed
  • [Journal Article] The molecular basis of CRL4DDB2/CSA ubiquitin ligase architecture, targeting, and activation2011

    • Author(s)
      Fischer ES, Scrima A, Bohm K, Matsumoto S, Lingaraju GM, Faty M, Yasuda T, Cavadini S, Wakasugi M. Hanaoka F, Iwai S, Gut H, Sugasawa K, Thoma NH.
    • Journal Title

      Cell

      Volume: 147 Pages: 1024-1039

    • DOI

      doi:10.1016/j.cell.2011.10.035

    • Peer Reviewed
  • [Journal Article] 休止期におけるヌクレオチド除去修復依存的な二次的DNA損傷の生成2011

    • Author(s)
      若杉光生, 松永司
    • Journal Title

      放射線生物研究

      Volume: 46 Pages: 322-331

    • URL

      http://www.naramed-u.ac.jp/~oncra/rbrc/

  • [Journal Article] Role of interaction of XPF with RPA in nucleotide excision repair2011

    • Author(s)
      Fisher LA, Bessho M, Wakasugi M, Matsunaga T, Bessho T.
    • Journal Title

      J Mol Biol

      Volume: 413 Pages: 337-346

    • DOI

      doi:10.1016/j.jmb.2011.08.034

    • Peer Reviewed
  • [Journal Article] SCFβ(TrCP) mediates stress-activated MAPK-induced Cdc25B degradation2011

    • Author(s)
      Uchida S, Watanabe N, Kudo Y, Yoshioka K, Matsunaga T, Ishizaka Y, Nakagama H, Poon RY, Yamashita K.
    • Journal Title

      J Cell Sci

      Volume: 124 Pages: 2816-2825

    • DOI

      doi:10.1242/jcs.083931

    • Peer Reviewed
  • [Journal Article] Physical and functional interaction between DDB and XPA innucleotide excision repair2009

    • Author(s)
      Wakasugi M, Kasashima H, Fukase Y, Imura M, Imai R, Yamada S, Cleaver JE, Matsunaga T.
    • Journal Title

      Nucleic Acids Res

      Volume: 37 Pages: 516-525

    • DOI

      doi:10.1093/nar/gkn964

    • Peer Reviewed
  • [Journal Article] Stress-activated mitogen-activated protein kinases c-Jun NH2-terminal kinase and p38 target Cdc25B for degradation2009

    • Author(s)
      Uchida S, Yoshioka K, Kizu R, Nakagama H, Matsunaga T, Ishizaka Y, Poon RY, Yamashita K.
    • Journal Title

      Cancer Res

      Volume: 69 Pages: 6438-44

    • DOI

      doi:10.1158/0008-5472.CAN09-0869

    • Peer Reviewed
  • [Presentation] ヌクレオチド除去修復能の新規解析系の開発とその応用2012

    • Author(s)
      松永司
    • Organizer
      国立遺伝学研究所研究集会
    • Place of Presentation
      国立遺伝学研究所(静岡県)
    • Year and Date
      2012-02-10
  • [Presentation] 休止期のヌクレオチド除去修復に伴う二重鎖切断の生成とATMシグナリング経路の活性化2011

    • Author(s)
      若杉光生
    • Organizer
      第34回日本分子生物学会年会
    • Place of Presentation
      パシフィコ横浜(神奈川県)
    • Year and Date
      2011-12-15
  • [Presentation] 休止期細胞におけるヌクレオチド除去修復に依存した二重鎖切断の生成とATMシグナリング経路の活性化2011

    • Author(s)
      若杉光生
    • Organizer
      日本放射線影響学会第54回大会
    • Place of Presentation
      神戸商工会議所会館(兵庫県)
    • Year and Date
      2011-11-18
  • [Presentation] ヒトG0期細胞におけるヌクレオチド除去修復に伴うATRとATMの活性化2010

    • Author(s)
      松永司
    • Organizer
      第33回日本分子生物学会年会・第83回日本生化学会大会
    • Place of Presentation
      神戸国際展示場(兵庫県)
    • Year and Date
      2010-12-09
  • [Presentation] ユビキチンE3リガーゼコンポーネントとしてのDDB1の機能解析2010

    • Author(s)
      若杉光生
    • Organizer
      第33回日本分子生物学会年会・第83回日本生化学会大会
    • Place of Presentation
      神戸国際展示場(兵庫県)
    • Year and Date
      2010-12-08
  • [Presentation] Essential role of DDB1 as a component of DDB1-CUL4 ubiquitin E3 ligase2010

    • Author(s)
      Kawaguchi, Y.
    • Organizer
      7th 3R(Replica-tion, Recombination and Repair) Symposium
    • Place of Presentation
      Toyama international conference center(Toyama)
    • Year and Date
      2010-10-29
  • [Presentation] 紫外線照射されたG0期細胞におけるヌクレオチド除去修復に依存した二次的DNA損傷の生成2010

    • Author(s)
      若杉光生
    • Organizer
      日本放射線影響学会第53回大会
    • Place of Presentation
      京都テルサ(京都府)
    • Year and Date
      2010-10-21
  • [Presentation] DDB1-Cul4 E3リガーゼの機能解析2010

    • Author(s)
      松永司
    • Organizer
      国立遺伝学研究所研究集会
    • Place of Presentation
      国立遺伝学研究所(静岡県)
    • Year and Date
      2010-10-01
  • [Presentation] 末梢血由来リンパ球を用いたヌクレオチド除去修復能診断系の開発2010

    • Author(s)
      松永司
    • Organizer
      第69回日本癌学会学術総会
    • Place of Presentation
      大阪国際会議場(大阪府)
    • Year and Date
      2010-09-24
  • [Presentation] DNA損傷によるChk1キナーゼの活性化とその制御2010

    • Author(s)
      松永司
    • Organizer
      平成22年度専門研究会
    • Place of Presentation
      京都大学原子炉実験所(大阪府)
    • Year and Date
      2010-09-11
  • [Presentation] Modulation ofnucleotide excision repair in human cells2010

    • Author(s)
      Wakasugi, M.
    • Organizer
      International Confernece on Radiation and Cancer Biology at Nagasaki 2010
    • Place of Presentation
      Matsumoto Ryojun Auditorium Bawdwin Hall(Nagasaki)
    • Year and Date
      2010-02-18
  • [Presentation] Histone H2AX phosphorylation triggered by nucleotide excision repair intermediates in quiescent cells2009

    • Author(s)
      Matsunaga, T.
    • Organizer
      Keystone Symposia "Telomere Biology and DNA Repair"
    • Place of Presentation
      RACV Royal Pines Resort(Australia)
    • Year and Date
      2009-10-11
  • [Presentation] The role of caspase-Dependent Chk1 cleavage during apoptosis2009

    • Author(s)
      Matuura, K.
    • Organizer
      Telomere Biology and DNA Repair
    • Place of Presentation
      RACV Royal Pines Resort(Australia)
    • Year and Date
      2009-10-11
  • [Presentation] 癌細胞におけるDNA損傷/複製ストレス後のChk1リン酸化の異常亢進2009

    • Author(s)
      松永司
    • Organizer
      第68回日本癌学会学術総会
    • Place of Presentation
      パシフィコ横浜(神奈川県)
    • Year and Date
      2009-10-01
  • [Remarks]

    • URL

      http://www.p.kanazawa-u.ac.jp/~iden/

  • [Patent(Industrial Property Rights)] DNA損傷修復能力の簡便・迅速な検査方法2010

    • Inventor(s)
      松永司、西山千晶
    • Industrial Property Rights Holder
      金沢大学
    • Industrial Property Number
      特許、特願2010-155303
    • Filing Date
      2010-07-07

URL: 

Published: 2013-07-31  

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