2011 Fiscal Year Final Research Report
Structural analysis of the interaction between the Ku protein and DNA
| Project/Area Number |
21510063
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Risk sciences of radiation/Chemicals
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| Research Institution | National Institute of Infectious Diseases |
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Principal Investigator |
FUJIMOTO Hirofumi 国立感染症研究所, 放射能管理室, 主任研究官 (60373396)
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| Co-Investigator(Kenkyū-buntansha) |
KOIKE Manabu 独立行政法人放射線医学総合研究所, 放射線障害研究グループ, 主任研究員 (70280740)
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| Co-Investigator(Renkei-kenkyūsha) |
SAKUDOH Takashi 国立感染症研究所, 放射能管理室, 主任研究官 (70455393)
TSUCHIDA Kozo 国立感染症研究所, 放射能管理室, 室長 (40231435)
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| Project Period (FY) |
2009 – 2011
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| Keywords | DNA二本鎖切断 / Ku / DNA損傷認識 / 分子動力学シミュレーション |
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| Research Abstract |
The binding mechanism of the repair enzyme, Ku, to the end of double strand DNA was examined by both computational and experimental techniques. Molecular models of Ku-DNA complex were constructed to estimate the binding energy between Ku and DNA, and amino acids that would interact with both DNA and Ku itself were identified. The new Ku-deficient cell line was established to investigate a detailed function of the predicted region.
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[Journal Article] A possible overestimation of the effect of acetylation on lysine residues in KQ mutant analysis2012
Author(s)
Hirofumi Fujimoto, Mariko Higuchi, Manabu Koike, Hirotaka Ode, Miroslav Pinak, Juraj Kotulic Bunta, Toshiyuki Nemoto, Takashi Sakudoh, Naoko Honda, Hideaki Maekawa, Kimiaki Saito, Kozo Tsuchida
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Journal Title
J. Comput. Chem
Volume: 33
Pages: 239-246
DOI
Peer Reviewed
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