2011 Fiscal Year Final Research Report
Bacterial heavy metal transporter MerC increases mercury accumulation in Arabidopsis thaliana.
| Project/Area Number |
21510088
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Environmental technology/Environmental materials
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| Research Institution | Kitasato University |
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Principal Investigator |
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| Project Period (FY) |
2009 – 2011
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| Keywords | メチル水銀トランスポーター / MerE / MerF / メチル水銀浄化 / ファイトレメディエーション / 土壌汚染浄化 |
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| Research Abstract |
This study evaluated the feasibility of transgenic Arabidopsis engineered to express the bacterial heavy metal transporter MerC for the phytoremediation of mercury pollution. MerC, MerC-SYP121, or MerC-AtVAM3 proteins were found to be expressed in leaf segments of transgenic plants using an anti-MerC antibody immunostaining method. Transgenic Arabidopsis plants expressing merC, merC-SYP121, and merC-AtVAM3 were more resistant to mercury and accumulated significantly more of this metal compared with wild-type Arabidopsis. These results demonstrated that the expression of the bacterial heavy metal transporter MerC can promote the transport and accumulation of mercury in transgenic Arabidopsis, which represents a method of improving plants for the phytoremediation of mercury pollution.
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[Journal Article] Expression of the bacterial heavy metal transporter MerC fused with a plant SNARE, SYP121, in Arabidopsis thaliana increases cadmium accumulation and tolerance2012
Author(s)
Kiyono M, Oka Y, Sone Y, Tanaka M, Nakamura R, Sato MH, Pan-Hou H, Sakabe K, Inoue K
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Journal Title
Planta
Volume: 235(4)
Pages: 841-850
Peer Reviewed
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