2011 Fiscal Year Final Research Report
Mechanism of U1 snRNP-independent splicing
| Project/Area Number |
21510204
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
基礎ゲノム科学
|
|---|
| Research Institution | Kobe University |
|---|
Principal Investigator |
INOUE Kunio 神戸大学, 大学院・理学研究科, 教授 (40252415)
|
|---|
| Project Period (FY) |
2009 – 2011
|
| Keywords | ゲノム発現 / スプライシング / イントロン / スプライス部位 / U1 snRNP / 熱ストレス |
|---|
| Research Abstract |
Many eukaryotic genes are interrupted by introns that are removed from primary transcripts by RNA splicing. U1 snRNP plays a crucial role in the 5' splice site recognition during splicing. Recently, we found the first example of naturally-occurring U1-independent U2-type splicing in humans. Our present study showed that a tissue-specific splicing regulator, Fox-1, failed to induce hF1γexon 9 skipping if the 5' splice site of intron 9 was mutated to the U1-dependent type, suggesting that U1-independent splicing contributes to the regulation of alternative splicing of a class of pre-mRNAs.
|
