2011 Fiscal Year Final Research Report
Bioinformatic and molecular biological study on ultra-short introns
| Project/Area Number |
21510205
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
基礎ゲノム科学
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| Research Institution | Fujita Health University |
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Principal Investigator |
SHIMADA Makoto 藤田保健衛生大学, 総合医科学研究所, 講師 (00528044)
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| Co-Investigator(Renkei-kenkyūsha) |
MAYEDA Akila 藤田保健衛生大学, 総合医科学研究所, 教授 (50212204)
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| Research Collaborator |
SASAKI Noriko (HARAGUCHI Noriko) 藤田保健衛生大学, 総合医科学研究所, 博士研究員 (90546649)
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| Project Period (FY) |
2009 – 2011
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| Keywords | ゲノム進化・再編 / イントロン長 / pre-mRNA splicing / ヒトゲノム / データベース |
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| Research Abstract |
The conspicuous cluster with a steep peak in length distribution have been found in the most eukaryotes ; however, there is no information about introns shorter than the cluster. We identified pre-mRNA introns shorter than the cluster, termed ultra-short introns.
We selected 23 of the ultra-short introns based on conservation status in closely related species. Using bioinformatics and molecular biological approaches, we demonstrated that the ultra-short introns are bona fide introns and the ultra-short introns independently appeared in closely related lineages to human. Our analyses on the features of the ultra-short introns suggest that the ultra-short introns are spliced by distinct multiple splicing mechanisms that are modified from the known conventional splicing mechanism.
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