2011 Fiscal Year Final Research Report
Applications of solid phase for photoaffinity labeling
| Project/Area Number |
21510219
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Living organism molecular science
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| Research Institution | Hokkaido University |
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Principal Investigator |
HASHIMOTO Makoto 北海道大学, 大学院・農学研究院, 准教授 (90292094)
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| Project Period (FY) |
2009 – 2011
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| Keywords | 光アフィニティーラベル / ジアジリン / アビジン-ビオチン / 固層担体 / 有機化学反応 |
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| Research Abstract |
Novel functional analysis of pinteractions between biomolecules and bioactive compounds with photoreactive trifluoromethylphenyldiazirine containing biotin derivative and immobilized monomeric avidin were examined. New findings are listed below.
(1) Synthesis of photoreactive biotinylated compounds
(2) Photoaffinity labeling with photoreactive biotinylated ligands on immobilized monomeric avidin.
(3) Novel synthesis of biotinylated compounds, which was prepared by Friedel-Crafts reactions.
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