2011 Fiscal Year Final Research Report
The structural determination and anti-angiogenic activity of a Chondromodulin-I subtype that lacks the N-terminal domain.
| Project/Area Number |
21510224
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Living organism molecular science
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| Research Institution | Kyoto University |
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Principal Investigator |
HIRAKI Yuji 京都大学, 再生医科研究所, 教授 (40144498)
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| Project Period (FY) |
2009 – 2011
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| Keywords | 血管新生抑制因子 / コンドロモジュリン.I / 抗腫瘍血管新生因子 / 軟骨細胞外マトリックス / プロテオリシス / 活性発現の分子機構 |
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| Research Abstract |
In this study, we extracted and purified the 14-kDa species of ChM-I from rat rib cartilage. Amino acid sequencing revealed a 14-kDa ChM-I lacks the N-terminal 37 residues of mature 25-kDa ChM-I. As demonstrated in vitro, 14-kDa ChM-I exhibited little anti-angiogenic activity. There is no 25-kDa ChM-I species present in the hypertrophic/calcified cartilage zone of cartilaginous bone precursors. Instead only the 14-kDa ChM-I species was detected. Taken together, our data suggest that ChM-I is inactivated by its N-terminal deletion of 37 residues during endochondral bone formation.
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