2011 Fiscal Year Final Research Report
Enhancing the practicability of the method for proteome analysis based on a new isolation scheme of C-terminal peptides.
| Project/Area Number |
21510225
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Living organism molecular science
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| Research Institution | Nara Women's University |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
KUYAMA Hiroki 大阪大学, 蛋白質研究所寄付研究部門, 特任准教授 (60437332)
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| Project Period (FY) |
2009 – 2011
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| Keywords | タンパク質 / C末端ペプチド / プロテオミクス / アミノ酸配列解析 / 質量分析 |
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| Research Abstract |
We have established a method to identify proteins by MALDI mass spectrometry of the C-terminal peptide obtained from a protein in which all the carboxyl groups were protected by exhaustive amidation. The optimized protocol to concentrate or isolate the C-terminal peptide from the proteolytic digest of the fully amidated protein includes the use of a strong anion-exchange HPLC column to remove anionic peptides.
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Research Products
(12 results)
