Functional analyses of cellulase genes in multicellular microbe and application to a new technology

2011 Fiscal Year Final Research Report

• Project/Area Number: 21510230
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Living organism molecular science
• Research Institution: Toho University
• Principal Investigator: KAWATA Takefumi 東邦大学, 理学部, 教授 (30221899)
• Project Period (FY): 2009 – 2011
• Keywords: セルラーゼ / 細胞壁 / 形態形成 / バイオマス / 転写因子 / 細胞性粘菌

Research Abstract

Cellulose is a major component of the cell wall for cellular slime mould, Dictyostelium discoideum. It posses a cellulose synthetase gene and its function is important for normal morphogenesis. In this project, we focused on the cellulase genes catalyzing cellulose hydrolysis, cloned several of them and investigated the effects on morphogenesis as well as cellulose hydrolyzing activity. We showed inactivation of cellobiohydrolase(cbhA) gene, one of cellulase, by gene disruption affected morphogenesis. In addition, multiple Dictyostelium cellulases might act synergistically, possibly as a cellulosome, resulted in drastic increase of cellulose hydrolyzing activity. This implies Dictyostelium is a useful organism to produce bioethanol from cellulose.

Research Products

• [Journal Article] STAT signaling in Dictyostelium development (2011)
  • Author(s): Kawata, T
  • Journal Title: Develop. Growth & Differ Volume: 53巻 Pages: 548-557
  • DOI: DOI:10.1111/j.1440-169X.2010.01243.x
  • Peer Reviewed
• [Journal Article] Evidence for a functional link between Dd-STATa and Dd-PIAS, a Dictyostelium PIAS homologue (2011)
  • Author(s): Kawata, T., Hirano, T., Ogasawara, S., Aoshima, R. and Yachi, A.
  • Journal Title: Develop. Growth & Differ Volume: 53巻 Pages: 897-909
  • DOI: DOI:10.1111/j.1440-169X.2011.01296.x
  • Peer Reviewed
• [Journal Article] The prespore cell inducing factor, psi factor(PsiA), controls both prestalk and prespore gene expression in Dictyostelium discoideum (2010)
  • Author(s): Yamada, Y. Minamisawa, H., Fukuzawa, M., Kawata, T. and Oohata, A. A.
  • Journal Title: Develop. Growth & Differ Volume: 52巻 Pages: 377-383
  • DOI: DOI:10.1111/j.1440-169X.2010.01177.x
  • Peer Reviewed
• [Journal Article] SunB, a novel SUN domain-containing protein required for development of Dictyostelium discoideum (2010)
  • Author(s): Shimada, N., Inouye, K., Sawai, S. and Kawata, T
  • Journal Title: Develop. Growth & Differ Volume: 52巻 Pages: 577-590
  • DOI: DOI:10.1111/j.1440-169X.2010.01189.x
  • Peer Reviewed
• [Presentation] TKL tyrosine kinase phosphorylates and activates a non-metazoan STAT (2011)
  • Author(s): Araki, T., Kawata, T. and Williams, J. G. A
  • Organizer: International Dictyostelium Conference
  • Place of Presentation: Baltimore, U. S. A.
  • Year and Date: 20110800
• [Presentation] Identification of two tyrosine kinase-like(TKL) proteins that redundantly activate STATc in response to hyper-osmotic stress (2011)
  • Author(s): Hai, L. V., Araki, T., Na, J., Kawata, T., Williams, J. G. and Eichinger, L.
  • Organizer: International Dictyostelium Conference
  • Place of Presentation: Baltimore, U. S. A.
  • Year and Date: 20110800
• [Presentation] Roles of SunB in centrosome positioning and nuclear division (2010)
  • Author(s): Shimada, N. Kawata, T. and Sawai, S
  • Organizer: 第33回日本分子生物学会年会、第83回日本生化学会大会合同大会
  • Place of Presentation: 神戸
  • Year and Date: 20101200
• [Presentation] Repression of Dd-STATa activity by Dd-PIAS, a Dictyostelium PIAS homologue (2010)
  • Author(s): Kawata, T., Hirano, T., Yachi, A., Ogasawara, S. and Aoshima, R.
  • Organizer: International Dictyostelium Conference
  • Place of Presentation: Cardiff, UK
  • Year and Date: 20100800
• [Presentation] Functional analysis and subcellular localization of SunB, a novel type of SUN protein (2010)
  • Author(s): Shimada, N., Kawata, T. and Sawai, S.
  • Organizer: International Dictyostelium Conference
  • Place of Presentation: Cardiff, UK
  • Year and Date: 20100800
• [Presentation] 細胞性粘菌における新規SUNドメインタンパク質SunBの細胞内局在解析 (2009)
  • Author(s): 島田奈央、谷口大相、川田健文、澤井哲
  • Organizer: 第12回細胞性粘菌研究会
  • Place of Presentation: 山口
  • Year and Date: 20091000
• [Presentation] 細胞性粘菌における新規SUNドメインタンパク質・sunBの機能解析 (2009)
  • Author(s): 島田奈央、川田健文、澤井哲
  • Organizer: 第8回核ダイナミクス研究会
  • Place of Presentation: 修善寺
  • Year and Date: 20090600
• [Remarks]
  • URL: http://www.lab.toho-u.ac.jp/sci/bio/moldev/d/cellulase2.htm