Basic studies on metabolic versatility of plant natural products and biosynthetic engineering with leguminous model systems

2011 Fiscal Year Final Research Report

• Project/Area Number: 21510233
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Living organism molecular science
• Research Institution: Nihon University
• Principal Investigator: AYABE Shin-ichi 日本大学, 生物資源科学部, 教授 (40050679)
• Co-Investigator(Kenkyū-buntansha): AOKI Toshio 日本大学, 生物資源科学部, 准教授 (80287606)
• Co-Investigator(Renkei-kenkyūsha): UCHIYAMA Hiroshi 日本大学, 生物資源科学部, 准教授 (40232871)
• Project Period (FY): 2009 – 2011
• Keywords: 二次代謝産物 / 植物形質転換 / フラボノイド / トリテルペノイド / ミヤコグサ / シオン / アカシア

Research Abstract

To clarify the mechanism that creates the vast structural diversity of plant natural products, cDNAs encoding the enzymes involved in the biosynthesis of flavonols of a leguminous model plant Lotus japonicus, a specific triterpenoid of Aster tataricus and polyphenols of Acacia mangium were cloned, and the enzyme reactions were analyzed. Also, metabolic engineering was attempted with L. japonicus hairy root systems in which the expression of genes of flavonoid pathway was altered. Furthermore, the phytoalexin response of the cultured L. japonicus cells against various microbe signals was analyzed in order to facilitate the engineering efficiencies.

Research Products

• [Journal Article] Molecular cloning of a cDNA encoding an oxidosqualene cyclase that yields shionone, a unique tetracyclic triterpene ketone of Aster tataricus (2011)
  • Author(s): Sawai, S., Uchiyama, H., Mizuno, S., Aoki, T., Akashi, T., Ayabe, S., Takahashi, T.
  • Journal Title: FEBS Letters Volume: 585 Pages: 1031-1036
  • Peer Reviewed
• [Journal Article] マメ科特異的5-デオキシ型イソフラボノイド生合成遺伝子の解析からわかってきたこと (2009)
  • Author(s): 青木俊夫、明石智義、内山寛、綾部真一
  • Journal Title: 植物の生長調節 Volume: 44 Pages: 31-42
  • Peer Reviewed
• [Presentation] マメ科ネムノキ亜科Acacia mangiumのカルコン異性化酵素遺伝子のクローニングと機能解析 (2011)
  • Author(s): 谷水和馬
  • Organizer: 第29回日本植物細胞分子生物学会大会
  • Place of Presentation: 九州大学箱崎キャンパス(福岡)
  • Year and Date: 2011-09-07
• [Presentation] Shionone synthase, an oxidosqualene cyclase of an Asteraceae plant that yields a unique tetracyclic triterpene ketone : molecular cloning and evolutionary view (2011)
  • Author(s): Ayabe, S., et al
  • Organizer: XVIII International Botanical Congress(第18回国際植物学会議)
  • Place of Presentation: Melbourne Congress and Exhibition Centre(オーストラリア・メルボルン)
  • Year and Date: 2011-07-29
• [Book] Plant Phenolics : Phenylpropanoids. In : Comprehensive Natural Products II : Chemistry and Biology (2010)
  • Author(s): Ayabe, S., Uchiyama, H., Aoki, T., Akashi, T.
  • Total Pages: 929-976