Studies on archaeal antioxidation system involving thioredoxin proteins

2011 Fiscal Year Final Research Report

• Project/Area Number: 21510237
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Living organism molecular science
• Research Institution: National Institute of Advanced Industrial Science and Technology
• Principal Investigator: NAKAMURA Tsutomu 独立行政法人産業技術総合研究所, 健康工学研究部門, 主任研究員 (10357668)
• Co-Investigator(Renkei-kenkyūsha): INOUE Tsuyoshi 大阪大学, 大学院・工学研究科, 教授 (20263204) ; MATSUMURA Hiroyoshi 大阪大学, 大学院・工学研究科, 准教授 (30324809) ; TAMADA Taro 日本原子力研究開発機構, 量子ビーム応用研究部門, 研究主幹 (50391248)
• Project Period (FY): 2009 – 2011
• Keywords: 活性発現の分子機構 / 活性酸素 / 抗酸化システム / 反応中間体

Research Abstract

In order to elucidate the evolution process of antioxidation system in life from the viewpoint of protein science, we structurally characterized archaeal peroxiredoxin(Prx) and related antioxidant proteins. Especially, structure determination of the complex of Prx and its substrate, hydrogen peroxide, enabled discussion on the very early stage and the transition state of the Prx reaction. Furthermore, we determined the 3D structure of an archaeal cambialistic superoxide dismutase, which revealed the relationship between metal selectivity and the local 3D structure of the enzyme. Elucidation of protein reaction mechanism involving hydrogen atoms needs collection of neutron diffraction data of the protein. We developed methods for obtaining large protein crystals for the purpose of the neutron diffraction study.

Research Products

• [Journal Article] Growth of protein crystals by syringe-type top-seeded solution growth (2011)
  • Author(s): Hiroyoshi Matsumura, Keisuke Kakiniuchi, Tsutomu Nakamura, Shigeru Sugiyama, Mihoko Maruyama, Hiroaki Adachi, Kazufumi Takano, Satoshi Murakami, Tsuyoshi Inoue, and Yusuke Mori
  • Journal Title: Cryst. Growth Des Volume: 11 Pages: 1486-1492
  • DOI: DOI:10.1021/cg101361n
• [Journal Article] Crystal structure of the cambialistic superoxide dismutase from Aeropyrum pernix K1 : insights into enzyme mechanism and stability (2011)
  • Author(s): Tsutomu Nakamura, Kasumi Torikai, Koichi Uegaki, Junji Morita, Kodai Machida, Atsushi Suzuki, Yasushi Kawata
  • Journal Title: FEBS J Volume: 278 Pages: 598-609
  • DOI: DOI:10.1111/j.1742-4658.2010.07977.x
• [Journal Article] Growth of large protein crystals by a large-scale hanging-drop method (2010)
  • Author(s): Keisuke Kakinouchi, Tsutomu Nakamura, Taro Tamada, Hiroaki Adachi, Shigeru Sugiyama, Mihoko Maruyama, Yoshinori Takahashi, KazufumiTakano, Satoshi Murakami, Tsuyoshi Inoue, Ryota Kuroki, Yusuke Mori and Hiroyoshi Matsumura
  • Journal Title: J. Appl. Cryst Volume: 43 Pages: 937-939
  • DOI: DOI:10.1107/S0021889810015967
• [Journal Article] Crystal structure of peroxiredoxin from Aeropyrum pernix K1 complexed with its substrate, hydrogen peroxide (2010)
  • Author(s): Tsutomu Nakamura, Yuji Kado, Takafumi Yamaguchi, Hiroyoshi Matsumura, Kazuhiko Ishikawa and Tsuyoshi Inoue
  • Journal Title: J. Biochem Volume: 147 Pages: 109-115
  • DOI: DOI:10.1093/jb/mvp154
• [Presentation] Crystal Structure of Archaeal Cambialistic Superoxide Dismutase (2011)
  • Author(s): Tsutomu Nakamura, Kasumi Torikai, Junji Morita, Yasushi Kawata, and Koichi Uegaki
  • Organizer: XXII Congress and General Assembly of the International Union of Crystallography
  • Place of Presentation: Madrid
  • Year and Date: 2011-08-27
• [Presentation] Aeropyrum pernix K1由来スーパーオキシドディスムターゼの立体構造解析 (2010)
  • Author(s): 中村努、鳥養香澄、上垣浩一、森田潤司
  • Organizer: 第10回日本蛋白質科学会年会
  • Place of Presentation: 札幌
  • Year and Date: 2010-06-17
• [Presentation] 古細菌ペルオキシレドキシンと過酸化水素複合体の立体構造 (2009)
  • Author(s): 中村努、門祐示、松村浩由、井上豪
  • Organizer: 第9回日本蛋白質科学会年会
  • Place of Presentation: 熊本
  • Year and Date: 2009-05-20