2011 Fiscal Year Final Research Report
Development of DNA-interstrand cross-linking compounds and application to DNA scaffold
| Project/Area Number |
21510239
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Living organism molecular science
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| Research Institution | National Institute of Advanced Industrial Science and Technology |
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Principal Investigator |
KOMATSU Yasuo 独立行政法人産業技術総合研究所, 生物プロセス研究部門, 研究グループ長 (30271670)
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| Project Period (FY) |
2009 – 2011
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| Keywords | 架橋 / DNA / RNA / アミノオキシ |
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| Research Abstract |
We have found that a single molecule containing bis(aminooxy) groups is capable of linking two AP sites, which are produced on "complementary" positions of double-stranded oligonucleotides. The aromatic molecule having two aminooxy groups showed much higher conjugation efficiency than the cross-linker with a straight alkyl chain. The interstrand cross-link(ICL) along with the stacking effect of the aromatic residue could stabilize the ICL duplex. We conducted two kinds of enzymatic reactions on the ICL-DNAs and showed that they have the potential to become stable nanosized scaffolds.
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[Journal Article] Interstrand cross-link of DNA by covalently linking a pair of abasic sites2012
Author(s)
Ichikawa, K., Kojima, N., Hirano, Y., Takebayashi, T., Kowata, K. and Komatsu, Y
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Journal Title
Chem. Commun.(Camb)
Volume: 48
Pages: 2143-2145
DOI
Peer Reviewed
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