2011 Fiscal Year Final Research Report
Intracellular protein delivery by high pressure modulation
| Project/Area Number |
21550154
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Chemistry related to living body
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| Research Institution | Kyoto Institute of Technology |
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Principal Investigator |
KUNUGI Shigeru 京都工芸繊維大学, 工芸科学研究科, 教授 (70111929)
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| Co-Investigator(Kenkyū-buntansha) |
TANAKA Naoki 京都工芸繊維大学, 工芸科学研究科, 准教授 (60243127)
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| Project Period (FY) |
2009 – 2011
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| Keywords | タンパク質 / 高圧 / 細胞内移送 / 塩基性ペプチド / 蛍光プローブ |
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| Research Abstract |
A novel method for the intracellular delivery of protein using high pressure modulation was developed. The protein transduction domain(PTD), a membrane permeable basic peptide, was conjugated with the antigen model protein ovalbumin(OVA). The heat treatment of the OVA with PTD peptide resulted in the formation of the protein nanoparticle with the zeta potential of-25 mV. On the other hand, the zeta potential of nanoparticle obtained by the pressure treatment at 400 MPa was-15 mV indicating that PTD peptide was localized on the surface of the protein nanoparticle. These results indicate that high pressure modulation is a useful method to improve the the cell uptake of protein nanoparticle.
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